1887

Abstract

SUMMARY: Sakacin A, a bacteriocin produced by Lb706 and which inhibits the growth of , was purified to homogeneity by ammonium sulphate precipitation and ion-exchange, hydrophobic-interaction and reversed-phase chromatography. The complete amino acid sequence of sakacin A was determined by Edman degradation. The bacteriocin consisted of 41 amino acid residues and had a calculated of 4308.7, which is in good agreement with the value determined by mass spectrometry. The structural gene encoding sakacin A () was cloned and sequenced. The gene encoded a primary translation product of 59 amino acid residues which was cleaved between amino acids 18 and 19 to yield the active sakacin A. Sakacin A shared some sequence similarities with other bacteriocins.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-12-2715
1992-12-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/12/mic-138-12-2715.html?itemId=/content/journal/micro/10.1099/00221287-138-12-2715&mimeType=html&fmt=ahah

References

  1. Axelsson L. T., Ahrné S. E. I., Andersson M. C. & Stahl S. R. ( 1988;). Identification and cloning of a plasmid-encoded erythromycin resistance determinant from. Lactobacillus reuteri. Plasmid 20, 171-174.
    [Google Scholar]
  2. Cornwell G. G., Sletten K., Johansson B. & Westermark P. ( 1988;). Evidence that the amyloid fibril protein in senile systemic amyloidosis is derived from normal prealbumin.. Biochemical and Biophysical Research Communications 154, 648-653.
    [Google Scholar]
  3. Cosby W. M., Axelsson L. T. & Dobrogosz W. J. ( 1989;). Tn917 transposition in Lactobacillus plantarum using the highly temperature-sensitive plasmid pTVJTs as a vector. . Plasmid 22, 236-243.
    [Google Scholar]
  4. Devereux J., Haeberli P. & Smithies O. ( 1984;). A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Research 12, 387-395.
    [Google Scholar]
  5. Dretzen G., Bellard M., Sassone-Corsi P. & Chambon P. ( 1981;). A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.. Analytical Biochemistry 112, 295-298.
    [Google Scholar]
  6. Geis A., Jasjit J. & Teuber M. ( 1983;). Potential oflactic streptococci to produce bacteriocin.. Applied and Environmental Microbiology 45, 205-211.
    [Google Scholar]
  7. Gross E. & Morell J. ( 1971;). The structure of nisin.. Journal of the American Chemical Society 93, 4634-4635.
    [Google Scholar]
  8. Hastings J. W., Sailer M., Johnson K., Roy K. L., Vederas J. C. & Stiles M. E. ( 1991;). Characterization of Leucocin A-UAL 187 and cloning of the bacteriocin gene from Leuconostoc gelidum. . Journal of Bacteriology 173, 7491-7500.
    [Google Scholar]
  9. Holo H., Nilssen O. & Nes I. F. ( 1991;). Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene. . Journal of Bacteriology 173, 3879-3887.
    [Google Scholar]
  10. Joerger M. C. & Klaenhammer T. R. ( 1990;). Cloning, expression, and nucleotide sequence of the Lactobacillus helveticus 481 gene encoding the bacteriocin Helveticin J. . Journal of Bacteriology 172, 6339-6347.
    [Google Scholar]
  11. Kok J., Nes I. F., How H., Van Belkum M. J. & Haandrikman A. ( 1992;). Non-nisin bacteriocins in lactococci: biochemistry, genetics and mode of action.. In Bacteriocins of Lactic Acid Bacteria, Edited by Hoover D. G. & Steenson L. R.. New York:: Academic Press (in the Press);.
    [Google Scholar]
  12. Man J. C. De. , Rogosa M. & Sharpe M. E. ( 1960;). A medium for the cultivation of lactobacilli.. Journal of Applied Bacteriology 23, 130-135.
    [Google Scholar]
  13. Muriana P. M. & Klaenhammer T. R. ( 1991;). Cloning, phenotypic expression, and DNA sequence of the gene for Lactacin F, an antimicrobial peptide produced by. Lactobacillus spp. Journal of Bacteriology 173, 1779-1788.
    [Google Scholar]
  14. Mørtvedt C. I. & Nes I. F. ( 1990;). Plasmid-associated bacteriocin production by a. Lactobacillus sake strain. Journal of General Microbiology 136, 1601-1607.
    [Google Scholar]
  15. Mørtvedt C. I., Nissen-Meyer J., Sletten K. & Nes I. F. ( 1991;). Purification and amino acid sequence of Lactocin S, a bacteriocin produced by Lactobacillus sake L45. . Applied and Environmental Microbiology 51, 1829-1834.
    [Google Scholar]
  16. Lozano Nieto. , Nissen-Meyer J., Sletten K., Pelaz C. & Nes I. F. ( 1992;). Purification and amino acid sequence of a bacterocin produced by Pediococcus acidilactici. . Journal of General Microbiology 138, 1985-1990.
    [Google Scholar]
  17. Nissen-Meyer J., Hold H., Havardstein L. S., Sletten K. & Nes I. F. ( 1992;). A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides.. Journal of Bacteriology 174, 5686-5692.
    [Google Scholar]
  18. Ojcius D. M. & Young J. D. -E. ( 1991;). Cytolytic pore-forming proteins and peptides: is there a common structural motif?. Trends in Biochemical Sciences 16, 225-229.
    [Google Scholar]
  19. RAO J. K. M. & ARGOS P. ( 1986;). A conformational preference parameter to predict helices in integral membrane proteins.. Biochimica et Biophysica Acta 869, 197-214.
    [Google Scholar]
  20. Sambrook J., Fritsch E. F. & Maniatis T. ( 1989). Molecular Cloning: a Laboratory Manual, , 2.nd edn. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  21. Sanger F., Nicklen S. & Coulson R. ( 1977;). DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 14, 5463-5467.
    [Google Scholar]
  22. Schillinger U. & Locke F. -K. ( 1989;). Antibacterial activity of Lactobacillus sake isolated from meat. . Applied and Environmental Microbiology 55, 1901-1906.
    [Google Scholar]
  23. Schillinger U., Kaya M. & Locke F. -K. ( 1991;). Behaviour of Listeria monocytogenes in meat and its control by a bacteriocinproducing strain of Lactobacillus sake. . Journal of Applied Bacteriology 70, 473-478.
    [Google Scholar]
  24. Sorensen H. H., Thompsen J., Bayne S., H0Jrup P. & Roepstorff P. ( 1990;). Strategies for determination of disulphide bridges in proteins using plasma desorption mass spectrometry.. Biomedical and Environmental Mass Spectrometry 19, 713-720.
    [Google Scholar]
  25. Tichaczek P. S., Nissen-Meyer J., Nes I. F., Vogel R. F. & Hammes W. P. ( 1992;). Characterization of the bacteriocins Curvacin A from Lactobacillus curvatus LTH1174 and Sakacin P from L. sake LTH673. . Systematic and Applied Microbiology (in the Press).
    [Google Scholar]
  26. Tsarbopoulos A. ( 1989;). Plasma desorbtion mass spectrometry of natural and recombinant peptides and proteins.. Peptide Research 2, 258-266.
    [Google Scholar]
  27. Van Belkum M. J., Hayema B. J., Jeeninga R. E., Kok J. & Venema G. ( 1991a;). Organization and nucleotide sequences of two lactococcal bacteriocin operons.. Applied and Environmental Microbiology 51, 492-498.
    [Google Scholar]
  28. Van Belkum M. J., Kok J., Venema G., How H., Nes I. F., Konings W. N. & Abee T. ( 1991b;). The bacteriocin Lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner.. Journal of Bacteriology 173, 7934-7941.
    [Google Scholar]
  29. Van Belkum M. J., Kok J. & Venema G. ( 1992;). Cloning, sequencing, and expression in Escherichia coli of lcnB, a third bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6. . Applied and Environmental Microbiology 58, 572-577.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-12-2715
Loading
/content/journal/micro/10.1099/00221287-138-12-2715
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error