1887

Abstract

SUMMARY: The surface properties of nine and four strains from the oral cavity were examined and compared with a large group of oral streptococci. Zeta potential and contact angle measurements were employed to determine physico-chemical cell surface properties. In addition, elemental surface concentration ratios were obtained via X-ray photoelectron spectroscopy, and surface structures were examined with transmission electron microscopy. The and strains were found to have higher isoelectric points, higher hydrophobicities and higher N/C surface concentration ratios than some other oral streptococci. The combined data suggest that both species possess large amounts of surface protein. All the strains displayed abundant surface fibrils in negative staining, but the strains were devoid of surface appendages indicating that surface protein is present in different forms in the two species. The surfaces of and type strains differed significantly from the other strains examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-12-2707
1992-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/12/mic-138-12-2707.html?itemId=/content/journal/micro/10.1099/00221287-138-12-2707&mimeType=html&fmt=ahah

References

  1. Abramson H. A., Moyer L. S. & Gorin M. H. ( 1942;). The Electrophoresis of Proteins and the Chemistry of Cell Surfaces. . New York:: Reinholz;.
    [Google Scholar]
  2. Amory D. E., Genet M. J. & Rouxhet P. G. ( 1988;). Application of XPS to the surface analysis of yeast cells.. Surface and Interface Analysis, 11, 478-486.
    [Google Scholar]
  3. Bain C. D. & Whitesides G. M. ( 1988;). Molecular-level control over surface order in self-assembled monolayer films of thiols on gold.. Science, 240, 62-63.
    [Google Scholar]
  4. Beighton D. ( 1984;). The influence of saliva on the hydrophobic surface properties of bacteria isolated from oral sites of macaque monkeys.. FEMS Microbiology Letters 21, 239-242.
    [Google Scholar]
  5. Bourgault A. M., Rosenblatt J. E. & Fitzgerald R. H. ( 1980;). Peptococcus magnus: a significant human pathogen. . Annals of Internal Medicine 93, 244-248.
    [Google Scholar]
  6. Busscher H. J., Weerkamp A. H., Van Der Mei H. C., Van Pelt A. W. J., De Jong H. P. & Arends J. ( 1984;). Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion.. Applied and Environmental Microbiology 48, 980-983.
    [Google Scholar]
  7. Busscher H. J. & Weerkamp A. H. ( 1987;). Specific and non-specific interactions in bacterial adhesion to solid substrata.. FEMS Microbiology Reviews 46, 165-173.
    [Google Scholar]
  8. Busscher H. J., Handley P. S., Rouxhet P. G., Hesketh L. M. & Van Der Mei H. C. ( 1991;). The relationship between structural and physicochemical surface properties of tufted Streptococcus sanguis strains. . In Microbial Cell Surface Analysis -Structural and Physicochemical methods, pp. 317-338. Edited by Mozes N., Handley P. S., Busscher H. J. & Rouxhet P. G.. New York:: VCH Publishers;.
    [Google Scholar]
  9. Hancock I. & Poxton I. . ( 1988;). Bacterial Cell. Surface Technique, pp. 281-286. Edited by Hancock I. & Poxton I.. Chichester:: Wiley;.
    [Google Scholar]
  10. Hardie I. M. & Marsh P. D. ( 1978;). Streptococci and the human oral flora.. In Streptococci, p. 162. Edited by Skinner F. A. & Quesnel L. B.. London:: Academic Press;.
    [Google Scholar]
  11. Hofstad T. ( 1985;). The classification and identification of the anaerobic Gram-positive cocci.. Scandinavian Journal of Infectious Diseases Supplement 46, 14-17.
    [Google Scholar]
  12. James A. M. ( 1982;). The electrical properties and topochemistry of bacterial cells.. Advances in Colloid and Interface Science 15, 171-221.
    [Google Scholar]
  13. Kilian M. Mikkelsen L. & Henrichsen J. ( 1989;). Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and 2714 M. M. Cowan and others Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrews and Horder 1906). . International Journal of Systematic Bacteriology 4, 471-484.
    [Google Scholar]
  14. Marsh P. D. & Martin M. V. ( 1980;). Aspects of Microbiology series.. In Oral Biology, , 2nd. edition., p. 14. Washington DC: American Society for Microbiology;.
    [Google Scholar]
  15. Millard M. M., Scherrer R. & Thomas R. S. ( 1976;). Surface analysis and depth profile composition of bacterial cells by X-ray photoelectron spectroscopy and oxygen plasma etching.. Biochemical and Biophysical Research Communications 72, 1209-1217.
    [Google Scholar]
  16. Moore W. E. C., Holdeman L. V., Smibert R. M., Hash D. E. & Ranney R. R. ( 1982;). Bacteriology of severe periodontitis in young adult humans.. Infection and Immunity 38, 1137-1148.
    [Google Scholar]
  17. Mozes N., Amory D. E., LeóNard A. J. & Rouxhet P. G. ( 1989;). Surface properties of microbial cells and their role in adhesion and flocculation.. Colloids and Surfaces 42, 313-329.
    [Google Scholar]
  18. Olsson J., Glantz P. O. & Krasse B. ( 1976;). Electrophoretic mobility of oral streptococci.. Archives of Oral Biology 21, 605-609.
    [Google Scholar]
  19. Pratt-Terpstra I. H., Mulder J., Weerkamp A. H., Feijen J. & Busscher H. J. ( 1991;). Secretory lgA adsorption and oral streptococcal adhesion to human enamel and artificial solid substrata with various surface free energies.. Journal of Biomaterials Science Polymer Edition 2, 239-253.
    [Google Scholar]
  20. Ratner B. D. & Mcelroy B. J. ( 1985;). Electron spectroscopy for chemical analysis: applications in the biomedical sciences.. In Spectroscopy in the Biomedical Sciences, pp. I07-140. Edited by Gendrau R. M.. CRC Press:.
    [Google Scholar]
  21. Rogosa M. ( 1971;). Peptococcaceae, a new family to include the Grampositive anaerobic cocci of the genera, Peptococcus, Peptostreptococcus, and Ruminococcus. . International Journal of Systematic Bacteriology 21, 234-237.
    [Google Scholar]
  22. Shinjo T., Hazu H. & Kiyoyama H. ( 1987;). Hydrophobicity of Fusobacterium necrophorum biovars A and B. . FEMS Microbiology Letters 48, 243-247.
    [Google Scholar]
  23. Silva-Filho F. C. E., Saraiva E. M. B., Santos M. A. V. & De Souza W. ( 1990;). The surface free energy of Leishmania mexicana amazonensis. . Cell Biophysics 17, 137-151.
    [Google Scholar]
  24. Slots J. ( 1979;). Subgingival microflora and periodontal disease.. Journal of Clinical Periodontology 6, 351-382.
    [Google Scholar]
  25. Socransky S. S. ( 1970;). Relationship of bacteria to the etiology of periodontal disease.. Journal of Dental Research 49, 203-222.
    [Google Scholar]
  26. Tam Y-C., Noble P. B. & Chan E. C. S. ( 1987;). Effects of extracellular products of a presumed gingival pathogen, the bacterium Peptostreptococcus 84H14S, on cultured human fibroblasts and Hela cells. . Archives of Oral Biology 4, 303-305.
    [Google Scholar]
  27. Ter Steeg P. F., Van Der Hoeven J. S., De Jong M. H., Van Munster P. J. J. & Jansen M. J. H. ( 1987;). Enrichment of subgingival microflora on human serum leading to accumulation of Bacteroides species, Peptostreptococci and Fusobacteria. . Antonie van Leeuwenhoek 53, 261-271.
    [Google Scholar]
  28. Van Der Mei H. C., LeóNard A. J., Weerkamp A. H., Rouxhet P. G. & Busscher H. J. ( 1988a;). Surface properties of Streptococcus salivarius HB and non-fibrillar mutants: zeta potential and elemental composition using X-ray photoelectron-spectroscopy. . Journal of Bacteriology 170, 2462-2466.
    [Google Scholar]
  29. Van Der Mei H. C., LeóNard A. J., Weerkamp A. H., Rouxhet P. G. & Busscher H. J. ( 1988b;). Properties of oral streptococci relevant for adherence: zeta potential, surface free energy and elemental composition.. Colloids and Surfaces 32, 297-305.
    [Google Scholar]
  30. Van Der Mei H. C., Brokke P., Dankert J., Feijen J., Rouxhet P. G. & Busscher H. J. ( 1989a;). Physicochemical surface properties of nonencapsulated and encapsulated coagulase-negative st△pHylococci.. Applied and Environmental Microbiology 55, 2806-2814.
    [Google Scholar]
  31. Van Der Mei H. C., Genet M. J., Weerkamp A. H., Rouxhet P. G. & Busscher H. J. ( 1989b;). A comparison between the elemental surface compositions and electrokinetic properties of oral streptococci with and without adsorbed salivary constituents.. Archives of Oral Biology 34, 889-894.
    [Google Scholar]
  32. Van Der Mei H. C., Desoet J. J., Oegraaff J., Rouxhet P. G. & Busscher H. J. ( 1991;). Comparison of the physicochemical surface properties of Streptococcus rattus with those of other mutans streptococcal species. . Caries Research 25, 415-423.
    [Google Scholar]
  33. Van Loosdrecht M. C. M., Lyklema J., Norde W., Schraa G. & Zehnder A. J. B. ( 1987;). Electrophoretic mobility and hydrophobicity as a measure to predict the initial steps of bacterial adhesion.. Applied and Environmental Microbiology 53, 1898-1901.
    [Google Scholar]
  34. Wicken A. J. & Knox K. W. ( 1983;). Cell surface amphiphiles of Gram-positive bacteria.. Toxicon, suppl. 3, 501-507.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-12-2707
Loading
/content/journal/micro/10.1099/00221287-138-12-2707
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error