1887

Abstract

SUMMARY: The mechanism by which strain 104-S adheres to porcine squamous epithelium was investigated by studying the adsorption to epithelial cells, and control surfaces, of radioactively labelled material released from the bacterial cells by water extraction. The released material was fractionated by gel filtration and the adsorption of pronase-sensitive and -resistant material in the various fractions to porcine gastric tissue and the control surfaces of polystyrene and immobilized bovine serum albumin (BSA) was determined. The fraction with affinity for the epithelium was characterized by enzymic degradation, periodate oxidation, lipid extraction, and protein and carbohydrate analyses. The adsorption pattern of radioactively labelled crude released material mimicked the adhesion of whole labelled cells to polystyrene and to gastric squamous tissue pieces. On fractionation, the pattern of adsorption to polystyrene and BSA was different from that obtained for the tissue pieces. Considerably less labelled pronase-stable material bound to surfaces of polystyrene and BSA, as compared with the tissue, suggesting that the pronase-resistant component has a tissue-specific affinity. After pronase treatment of the fraction of about 20000 (20 K) containing labelled components with affinity for the epithelium, only saccharides were detected. Radioactivity was lost after hydrolysis with HCl, and therefore this pronase-resistant labelled component must be a saccharide. It is concluded that protein moieties in the extract have an affinity for several surfaces, including polystyrene, and that saccharide moieties have a specific affinity for the gastric squamous epithelium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-12-2657
1992-12-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/12/mic-138-12-2657.html?itemId=/content/journal/micro/10.1099/00221287-138-12-2657&mimeType=html&fmt=ahah

References

  1. Barrow P. A., Brooker B. E., Fuller B. E. & Newport M. J. ( 1980;). The attachment of bacteria to the gastric epithelium of the pig and its importance in the microbiology of the intestine.. Journal of Applied Bacteriology 48, 147-154.
    [Google Scholar]
  2. Bradford M. ( 1976;). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding.. Analytical Biochemistry 12, 248-254.
    [Google Scholar]
  3. Brooker B. E. & Fuller R. ( 1975;). Adhesion of lactobacilli to the crop epithelium.. Journal of Ultrastructure Research 52, 21-31.
    [Google Scholar]
  4. Conway P. L. & Kjelleberg S. ( 1989;). Protein mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium. . Journal of General Microbiology 135, 1175-1186.
    [Google Scholar]
  5. Coyette J. & Ghuysen J. M. ( 1970;). Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser. . Biochemistry 9, 2935-2943.
    [Google Scholar]
  6. Dubois M., Gilles K. A., Hamilton J. K., Rebers P.A. & Smith F. ( 1956;). Colorimetric method for determination of sugars and related substances.. Analytical Chemistry 28, 350-356.
    [Google Scholar]
  7. Folch J., Lees M. & Sloane-Stanley G. H. ( 1957;). A simple method for the isolation and purification of total lipids from animal tissues.. Biological Chemistry 226, 497-509.
    [Google Scholar]
  8. Fuller R., Barrow P. A. & Brooker B. E. ( 1978;). Bacteria associated with the gastric epithelium of neonatal pigs.. Applied and Environmental Microbiology 35, 582-591.
    [Google Scholar]
  9. Henriksson A., Sezwzyk R. & Conway P. L. ( 1991;). Characteris-tics of the adhesive determinants of Lactobacillus fermentum 104. . Applied and Environmental Microbiology 57, 499-502.
    [Google Scholar]
  10. Jentoft N. & Dearborn D. G. ( 1979;). Labeling of proteins by reductive methylation using sodium cyanoborohydride.. Journal of Biological Chemistry 254, 4359-4365.
    [Google Scholar]
  11. Savage D. C. ( 1972;). Association and physiological interaction of indigenous microorganisms and gastrointestinal epithelia.. American Journal of Clinical Medicine 25, 1372-1379.
    [Google Scholar]
  12. Sherman L. & Savage D. C. ( 1986;). Lipoteichoic acids in Lactobacillus strains that colonize the mouse gastric epithelium. . Applied and Environmental Microbiology 52, 302-304.
    [Google Scholar]
  13. Smith W. H. ( 1965;). Observations on the flora of the alimentary tract of animals and factors affecting its composition.. Journal of Pathological Bacteriology 89, 95-122.
    [Google Scholar]
  14. Wadstr0M T., Andersson K., Sydow M., Axelsson L., Lindgren S. & Gullmar B. ( 1987;). Surface properties of lactobacilli isolated from the small intestine of pigs.. Journal of Applied Bacteriology 62, 513-520.
    [Google Scholar]
/content/journal/micro/10.1099/00221287-138-12-2657
Loading
/content/journal/micro/10.1099/00221287-138-12-2657
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error