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Volume 138, Issue 12

Identification of an outer-membrane haemoglobin-binding protein in Free

Abstract

SUMMARY: Although can use haemoglobin as an iron source , the mechanism of haemoglobin-iron uptake is unknown. Using a biotinylated human haemoglobin probe in a solid-phase dot-binding assay, haemoglobin-binding activity was detected in total membranes derived from meningococci grown under iron-limited but not iron-sufficient conditions. In competition binding experiments, bovine and human haemoglobin could abrogate binding. In contrast, no binding inhibition was seen with ferric nitrate, protoporphyrin IX, and iron-loaded human transferrin. The ability of both haemin and catalase, a nonhaemoglobin haem-containing compound, to inhibit binding competitively suggested that the ligand recognized by the binding protein is the haem moiety. Scatchard plot analysis revealed a heterogeneous receptor population. Limited proteolysis with proteinase K abolished binding activity, suggesting a haemoglobin-protein interaction. Detection of activity in a whole-cell binding assay demonstrated that this haemin-binding protein was surface exposed. In a limited survey of meningococcal strains, the presence of haemoglobin-binding activity in all isolates indicated that expression of this binding protein is not serogroup specific.

  • Received:
  • Accepted:
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  • Published Online:
© Society for General Microbiology, 1992
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1992-12-01
2024-04-19
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Identification of an outer-membrane haemoglobin-binding protein in Neisseria meningitidis
Microbiology 138, 2647 (1992); https://doi.org/10.1099/00221287-138-12-2647
/content/journal/micro/10.1099/00221287-138-12-2647
/content/journal/micro/10.1099/00221287-138-12-2647
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