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Volume 138, Issue 12

Structural studies of an emulsion-stabilizing exopolysaccharide produced by an adhesive, hydrophobic strain Free

Abstract

SUMMARY: The primary structure of an emulsion-stabilizing exopolysaccharide from the adhesive, hydrophobic strain No. 33 was elucidated by NMR spectroscopy, methylation analyses, periodate oxidation and oligosaccharide analyses. The polysaccharide PS-33 consisted of rhamnose, galactose, glucose and glucuronic acid in molar ratios of 2:1:1:1. The main chain contained 3-substituted α-D-glucuronic acid linked to the 3-position at α-L-rhamnose, in addition to 3-substituted residues of β-D-galactose and α-D-glucose. The α-L-rhamnose of the side chain was linked to position 4 of the galactose. In addition, the polysaccharide was -acetylated, corresponding to one acetyl group per repeating unit. From the results two structural possibilities could be suggested. As the polysaccharide carries hydrophobic groups (methyl of rhamnose/-acetyl), it is very likely that these are of general significance for the emulsifying activity of polysaccharides. It also seems to be possible that this polysaccharide is at least partially responsible for the hydrophobic cell surface properties of the strain No. 33 and it may be involved in hydrophobic interactions when adhering to hydrophobic interfaces.

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© Society for General Microbiology, 1992
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1992-12-01
2024-04-19
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Structural studies of an emulsion-stabilizing exopolysaccharide produced by an adhesive, hydrophobic Rhodococcus strain
Microbiology 138, 2531 (1992); https://doi.org/10.1099/00221287-138-12-2531
/content/journal/micro/10.1099/00221287-138-12-2531
/content/journal/micro/10.1099/00221287-138-12-2531
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