1887

Abstract

SUMMARY: Secondary alcohols of were studied by gas chromatography and gas chromatography-mass spectrometry. Mycobacterial cells were hydrolysed and the liberated alcohols separated by extraction and analysed both underivatized and as trimethylsilyl-, methyl ether- and pentafluorobenzoyl derivatives. Seven straight-chain secondary alcohols containing from 18 to 24 carbon atoms and two branched-chain secondary alcohols with 21 and 23 carbon atoms were present in all of the studied strains.

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/content/journal/micro/10.1099/00221287-138-12-2499
1992-12-01
2024-04-24
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References

  1. Alugupalli S., Mielniczuk Z. & Larsson L. ( 1992;). Gas chromatography-mass spectrometry methods for analysis of secondary alcohols present in the Mycobacterium avium complex. . Journal of Microbiological Methods 15, 229-240.
    [Google Scholar]
  2. Jantzen E., Tangen T. & Eng J. ( 1989;). Gas chromatography of rnycobacterial fatty acids and alcohols: diagnostic applications.. Acta Pathologica Microbiologica et lmmunologica Scandinavica 97, 1037-1045.
    [Google Scholar]
  3. Jimenez J. & Larsson L. ( 1986;). Heating cells in acid methanol for 30 min without freeze-drying provides adequate yields of fatty acids and alcohols for gas chromatographic characterization of mycobacteria.. Journal of Clinical Microbiology 24, 844-845.
    [Google Scholar]
  4. Karlsson K. A., Samuelsson B. E. & Steen G. O. ( 1973;). Improved 2502 S. Alugupalli and L. Larsson identification of monomethyl paraffin chain branching (close to the methyl end) of long-chain compounds by gas chromatography and mass spectrometry. . Chemistry and Physics of Lipids 11, 17-38.
    [Google Scholar]
  5. Larsson L., Jimenez J., Valero-Guillen P. L., Martin-Luengo F. & Kubin M. ( 1989;). Establishment of 2-docosanol as a cellular marker compound in the identification of Mycobacterium xenopi. . Journal of Clinical Microbiology 27, 2388-2390.
    [Google Scholar]
  6. Luquin M., Lopez F. & Ausina V. ( 1989;). Capillary gas chromatographic analysis of mycolic acid cleavage products, cellular fatty acids and alcohols of M. xenopi. . Journal of Clinical Microbiology 27, 1403-1406.
    [Google Scholar]
  7. Luquin M., Ausina V., Calahorra Lopez. , F., Belda F., Garcia Barcelo M., Celma C. & Prats G. ( 1991;). Evaluation of practical chromatographic procedures for identification of clinical isolates of mycobacteria.. Journal of Clinical Microbiology 29, 120-130.
    [Google Scholar]
  8. Minnikin D. E. ( 1982). Lipids, complex lipids, their chemistry, biosynthesis and roles. . In The Biology of Mycobacteria, vol. I, pp. 95-184. Edited by Ratledge C. & Stanford J. L.. London:: Academic Press;.
    [Google Scholar]
  9. Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M. & Magnusson M. ( 1985;). Mycolic acid patterns of some species of Mycobacterium. . Archives of Microbiology 139, 225-231.
    [Google Scholar]
  10. Toriyama S., Imaizumi S., Tomiyasu I., Masui M. & Yano I. ( 1982;). Incorporation of 180 into long-chain, secondary alcohols derived from ester mycolic acids in Mycobacterium phlei. . Biochimica et Biophysica Acta 712, 427-429.
    [Google Scholar]
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