1887

Abstract

Non-enterotoxigenic porcine strains belonging to the serogroup O115 have been associated with septicaemia and diarrhoea. Putative factors important in the pathogenicity of of serogroup O115 include fimbrial antigen F165, haemagglutination (MRHA), lipopolysaccharide, serum resistance, capsule and production of aerobactin. Using Tn transposon insertion mutagenesis, two classes of mutants were obtained from of serotype O115:F165 with respect to the phenotypic expression of fimbrial antigen F165 and MRHA of sheep erythrocytes: class I, F165MRHA, serum resistant; class II, F165MRHA, serum resistant. In a chicken lethality model, class I mutants were either virulent or of intermediate virulence, while class II mutants were of intermediate virulence. Alkaline phosphatase activity of class I and class II Tn mutants showed similar environmental regulation to that of fimbrial antigen F165. Moreover, class I and class II mutants were mutated in the -like locus, and lacked a 18.5 kDa and/or a 17.5 kDa fimbrial band.

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1992-11-01
2021-05-12
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References

  1. Aguero M. E., Cabello F. C. 1983; RelativecontributionofColV plasmid and K1 antigen to the pathogenicity of Escherichia coli. Infection and Immunity 40:359–368
    [Google Scholar]
  2. Birnboim H. C., DoLY J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic acids Research 7:1513–1523
    [Google Scholar]
  3. Broes A., Fairbrother J. M., Jacques M., Lariviere S. 1988; Isolation and characterization of a new fimbrial antigen (CS1541) from a porcine enterotoxigenic Escherichia coil O8: KX105 strain. FEMS Microbiology Letters 55:341–348
    [Google Scholar]
  4. Carbonetti N. H., Boonchai S., Parry S. H., Vaisanen-Rhen V., Korhonen I. K., Williams P. H. 1986; Aerobactin-mediated iron uptake by Escherichia coli isolates from human extraintestinal infections. Infection and Immunity 51:966–968
    [Google Scholar]
  5. Contrepois M., Fairbrother J.M., Kaura Y. K., Girardeau J.-P. 1989; Prevalence of CS31A and Fl65 surface antigens in Escherichia coli isolates from animals in France, Canada, and India. FEMS Microbiology Letters 59:319–324
    [Google Scholar]
  6. Coulton J. W., Reid G. K., Campana A. 1988; Export hybrid proteins FhuA′-Lacz and FhuA′-′PhoA to the cell envelope of Escherichia coli K-12. Journal of Bacteriology 170:2267–2275
    [Google Scholar]
  7. Fairbrother J.M., Lariviere S., Lallier R. 1986; New fimbrial antigen F165 from Escherichia coli serogroup 0115 strains isolated from piglets with diarrhea. Infection and Immunity 51:10–15
    [Google Scholar]
  8. Fairbrother J. M., Lariviere S., Johnson W. M. 1988a; Prevalence of fimbrial antigens and enterotoxins in nonclassical serogroups of Escherichia coli isolated from newborn pigs with diarrhea. American Journal of Veterinary Research 49:1325–1328
    [Google Scholar]
  9. Fairbrother J. M., Lallier R., Leblanc L., Jacques M., Lariviere S. 1988b; Production and purification of Escherichia coli fimbrial antigen Fl65. FEMS Microbiology Letters 56:247–252
    [Google Scholar]
  10. Fairbrother J.M., Broes A., Jacques M., Lariviere S. 1989; Pathogenicity of Escherichia coli 0115: KV165 isolated from pigs with diarrhea. American Journal of Veterinary Research 50:1029–1036
    [Google Scholar]
  11. Finlay B. B, Starnbach M. N., Francis C. L., Stocker B. A., Chatfield S., Dougan G., Falkow S. 1988; Identification and characterization of TnphoA mutants of Salmonella which are unable to pass through a polarized MOCK epithelial cell monolayer. Molecular Microbiology 2:757–766
    [Google Scholar]
  12. Girardeau J.P., Der Vartanian M., Ollier J. L., Contrepois M. 1988; CS31A, a new K88-related fimbrial antigen on bovine enterotoxigenic and septicemic Escherichia coli. Infection and Immunity 56:2180–2188
    [Google Scholar]
  13. Hacker J. 1989; Genetic determinants coding for fimbriae and adhesins of extra-intestinal Escherichia coli. Current Topics in Microbiology and Immunology, 151:1–28
    [Google Scholar]
  14. Hacker J., Schmidt G., Hughes C., Knapp S., Marget M., Goebel W. 1985; Cloning and characterization of genes involved in production of mannose-resistant, neuraminidase-susceptible (X) fimbriae from uropathogenic 06 :K15 :H31 Escherichia coli. Infection and Immunity 47:434–440
    [Google Scholar]
  15. Hacker J., Goebel W., Hof H., Konig W., Konig B., Scheffer J., Hughes C., Marre R. 1988 Adhesins, serum resistance and cytolysins of E. coli - genetic structure and role in pathogenicity. In Bacteria, Complement and the Phagocytic Cell, NATO ASI series H24 pp. 221–229 Edited by Cabello F. C., Pruzzo C. Berlin: Springer-Verlag;
    [Google Scholar]
  16. Harel J., Forget C., Saint-Amano J., Daigle F., Dubreuil D., Jacques M., Fairbrother J. M. 1992; Molecular cloning of a determinant coding for fimbrial antigen F1651, a Prs-like fimbrial antigen from porcine septicaemic Escherichia coli. Journal of General Microbiology 138:1495–1502
    [Google Scholar]
  17. Harel J., Daigle F., Maiti S., Desautels C., Labigne A., Fairbrother J.M. 1991a; Occurence of pap-, sfa-, and afa-related sequences among Fl65-positive Escherichia coli from diseased animals. FEMS Microbiology Letters 82:177–182
    [Google Scholar]
  18. Harel J., Lapointe H., Fallara A., Lortie L.-A., Bigras-Poulin M., Lariviere S., Fairbrother J.M. 1991b; Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea.. Journal of Clinical Microbiology 29:745–752
    [Google Scholar]
  19. Karr J. F., Nowicki B., Truong L. D., Hull R. A., Hull S. I. 1989; Purified P fimbriae from two cloned gene clusters of a single pyelonephritogenic strain adhere to unique structures in the human kidney. Infection and Immunity 57:3594–3600
    [Google Scholar]
  20. Korhonen T. K., Valtonen M. V., Parkkinen J., Vaisanen-Rhen V., Finne J., Ørskov F., Svenson S. B., Makela P.H. 1985; Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infection and Immunity 48:486–491
    [Google Scholar]
  21. Lindahl M., Faris A., Wadstr0M T., Hjerten S. 1981; A new test based on salting out to measure relative hydrophobicity of bacterial cells. Biochimica et Biophysica Acta 677:471–476
    [Google Scholar]
  22. Lund B., Marklund B.-I., Stromberg N., Lindberg F., Karls-Son K.-A., Normark S. 1988; Uropathogenic Escherichia coli express serologically identical pili with different receptor binding specificities. Molecular Microbiology 2:255–263
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Manoil C., Beckwith J. 1985; TnphoA : a transposon probe for protein export signals.. Proceedings of the National Academy of Sciences of the United States of America 828129–8133
    [Google Scholar]
  25. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  26. Miller I., Maskell D., Hormaeche C., Johnson K., Pickard D., Dougan G. 1989; Isolation of orally attenuated Salmonella typhimurium following TnphoA mutagenesis. Infection and Immunity 57:2758–2763
    [Google Scholar]
  27. Miller J. H. 1972 Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Miller V. L., Mekalanos J. J. 1988; A novel suicide vector and its use in construction of an insertion mutation: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. Journal of Bacteriology 170:2575–2583
    [Google Scholar]
  29. Morris J. A., Thorns C. J., Scoti A. C., Sojka W. J. 1982; Virulence properties associated with the Vir plasmid: a transmissible pathogenic characteristic associated with strains of invasive Escherichia coli. Journal of General Microbiology 128:2097–2103
    [Google Scholar]
  30. Ørskov I., Ørskov F. 1985; Escherichia coli in extraintestinal infections. Journal of Hygiene 95:551–575
    [Google Scholar]
  31. Pluschke G., Mayden J., Achtman M., Levine R. P. 1983; Role of the capsule and the O antigen in resistance of O18 : K 1 Escherichia coli to complement mediated killing. Infection and Immunity 42:907–913
    [Google Scholar]
  32. Reed L. J., Muench H. 1938; A simple method of estimation for fifty per cent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  33. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  34. Taylor P. W. 1988 The mode of C5b-9 attack on susceptible Gramnegative bacteria. In Bacteria, Complement, and the Phagocytic Cell, NATO ASI series H24 pp. 129–138 Edited by Cabello C. F., Pruzzo C. Berlin: Springer-Verlag;
    [Google Scholar]
  35. Taylor P. W., Kroll H. P. 1983; Killing of an encapsulated strain of Escherichia coli by human serum. Infection and Immunity 39:122–131
    [Google Scholar]
  36. Taylor R. K., Miller V. L., Furlong D. B., Mekalanos J. J. 1987; Use of phoA fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proceedings of the National Academy of Sciences of the United States of America 842833–2837
    [Google Scholar]
  37. Taylor R. K., Shaw C., Peterson K., Spears P., Mekalanos J. J. 1988; Safe, live Vibrio cholerae vaccines?. Vaccine 6:151–154
    [Google Scholar]
  38. Taylor R. K., Manoil C., Mekalanos J. J. 1989; Broad-host range vectors for delivery of TnphoA: use in genetic analysis of secreted determinants of Vibrio cholerae. Journal of Bacteriology 171:1870–1878
    [Google Scholar]
  39. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
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