Lipopolysaccharide heterogeneity in isolates from cattle and sheep Free

Abstract

Lipopolysaccharide (LPS) from 40 isolates of , comprising 23 serotype A1, seven serotype A2, one serotype T4, one serotype T10 and eight untypable isolates, obtained from diseased and healthy cattle or sheep, was characterized by SDS-PAGE and Western blotting. Ten different SDS-PAGE LPS profiles, five smooth and five rough, were identified among the biotype A and untypable isolates and designated LPS types 1–10. LPS types 1 and 2 were smooth, had similar O-antigen banding-patterns but differed in the low-molecular-mass or core-oligosaccharide regions; type 3 LPS was rough but had a core-oligosaccharide region similar to that of LPS type 1. No similarities were observed between these LPS types and types 6, 7 and 9, which were smooth, and types 4, 5, 8 and 10, which were rough. Most serotype A1 isolates (19/23) were of LPS type 1, whereas two isolates each had LPS of types 2 and 3. The majority (5/7) of serotype A2 isolates possessed type 3 LPS, whereas the remaining two isolates each had LPS of types 4 and 5. There was much greater heterogeneity within the untypable group of isolates, which comprised LPS of types 1 and 9 (two isolates each), and 6, 7, 8 or 10 (one isolate each). Western blotting analysis demonstrated that LPS types 1 and 2 had immunologically identical O-antigen side-chains but differed in their core-oligosaccharide regions, whereas the core-oligosaccharide region of rough LPS type 3 was immunologically very similar to that of LPS type 1. The other LPS types were immunologically unrelated to these three LPS types. The majority (20/23) of serotype A1 isolates originated from cattle and possessed LPS types 1 or 2, different from most (5/7) of the serotype A2 isolates which originated from sheep and possessed LPS of types 3 or 4. However, two of the three ovine serotype A1 isolates had the same type 3 LPS as occurred in most of the ovine serotype A2 isolates, suggesting a possible correlation between LPS type and host specificity. This study has demonstrated that LPS diversity within different serotypes of is greater than was previously thought and that certain LPS types might be host-specific.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-10-2185
1992-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/10/mic-138-10-2185.html?itemId=/content/journal/micro/10.1099/00221287-138-10-2185&mimeType=html&fmt=ahah

References

  1. Aarsleff B. E., , Biberstein L., , Shreeve B. J., & Thompson D. A. 1970; A study of untypable strains of Pasteurella haemolytica . Journal of Comparative Pathology 80:493–498
    [Google Scholar]
  2. Adlam C. 1989 The structure, function and properties of cellular and extracellular components of Pasteurella haemolytica . In Pasteur-ella and Pasteurellosis, pp. 75–92 Edited by Adlam C., & person-group-type="editor"> Rutter M. J. London: Academic Press;
    [Google Scholar]
  3. Aspinall G. O., , Mcdonald A. G., , Raju T. S., , Pang H., , Mills S. D., , Kurjanczyk L. A., & Penner J. L. 1992; Serological diversity and chemical structures of Campylobacter Jejuni low-molecular-weight lipopolysaccharides. Journal of Bacteriology 174:1324–1332
    [Google Scholar]
  4. Biberstein E. L. 1978; Biotyping and serotyping of Pasteurella haemolytica . Methods in Microbiology 10:252–269
    [Google Scholar]
  5. Biberstein E. L., & Francis C. K. 1968; Nucleic acid homologies between the A and T types of Pasteurella haemolytica . Journal of Medical Microbiology 1:105–108
    [Google Scholar]
  6. Biberstein E. L., , Gills M., & Knight H. 1960; Serological types of Pasteurella haemolytica . Cornell Veterinarian 50:283–300
    [Google Scholar]
  7. Bisgaard M., & Mutters R. 1986; Re-investigations of selected bovine and ovine strains previously classified as Pasteurella haemolytica and description of some new taxa within the Pasteurella haemolytica-complex . Acta Pathologica Microbiologica et Immunologica Scandinavica B 94:185–193
    [Google Scholar]
  8. Carter G. R. 1967; Pasteurellosis: Pasteurella multocida and Pasteurella haemolytica . Advances in Veterinary Science 11:321–379
    [Google Scholar]
  9. Chengappa M. M., , Carter G. R., & Bailie W. E. 1984; Comparison of indirect haemagglutination and rapid plate agglutination tests with counterimmunoelectrophoresis for typing Pasteurella haemolytica . Journal of Clinical Microbiology 20:660–663
    [Google Scholar]
  10. Davies R. L., , Ali Q., , Parton R., , Coote J. G., , Gibbs A., & Freer J. H. 1991; Optimal conditions for the analysis of Pasteurella haemolytica lipopolysaccharide by sodium dodecyl sulphate-poly-acrylamide gel electrophoresis. FEMS Microbiology Letters 90:23–28
    [Google Scholar]
  11. Donachie W., , Fraser J., , Quirie M., & Gilmour N. J. L. 1984; Studies on strains of Pasteurella haemolytica not typable by the indirect haemagglutination test. Research in Veterinary Science 37:188–193
    [Google Scholar]
  12. Durham J. A., , Antone S. M., , Cunningham M. W., & Confer A. W. 1988; Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: demonstration of antigenic similari-ties among several serotypes. Journal of Clinical Microbiology 26:885–889
    [Google Scholar]
  13. Emau P., , Giri S. N., & Bruss M. L. 1987; Effects of smooth and rough Pasteurella haemolytica lipopolysaccharides on plasma cyclic-nucleotides and free fatty acids in calves. Veterinary Microbiology 15:279–292
    [Google Scholar]
  14. Fodor L., , Vara J., , Hajtos J., , Donachie W., & Gilmour N. J. L. 1988; Characterization of a new serotype of P. haemolytica isolated in Hungary. Research in Veterinary Science 44:399
    [Google Scholar]
  15. Frank G. H. 1980; Serological groups among untypable bovine isolates of Pasteurella haemolytica . Journal of Clinical Microbiology 12:579–582
    [Google Scholar]
  16. Frank G. H., & Wessman G. E. 1978; Rapid plate agglutination procedure for serotyping Pasteurella haemolytica . Journal of Clinical Microbiology 7:142–145
    [Google Scholar]
  17. Gilmour N. J. L., & Gilmour J. S. 1989 Pasteurellosis of sheep. In Pasteurella and Pasteurellosis, pp. 223–262 Edited by Adlam C., & person-group-type="editor"> Rutter M. J. London: Academic Press;
    [Google Scholar]
  18. Hackstadt T., , Peacock M. G., , Hitchcock P. J., & Cole R. L. 1985; Lipopolysaccharide variation in Coxiella burnetii: intra-strain heterogeneity in structure and antigenicity. Infection and Immunity 48:359–365
    [Google Scholar]
  19. Hitchcock P. J., & Brown T. M. 1983; Morphological hetero-geneity among Salmonella lipopolysaccharides chemotypes in silver-stained polyacrylamide gels. Journal of Bacteriology 154:269–277
    [Google Scholar]
  20. Hitchcock P. J., , Leive L., , Makela H., , Rietschel E.T., , Stritt-Matter W., & Morrison D. C. 1986; Lipopolysaccharide nomenclature-past, present and future. Journal of Bacteriology 166:699–705
    [Google Scholar]
  21. Inzana T. J. 1983; Electrophoretic heterogeneity and interstrain variation of the Iipopolysaccharide of Haemophilus injluenzae . Journal of Infectious Diseases 148:492–499
    [Google Scholar]
  22. Karch H., , Leying H., & Opferkuch W. 1984; Analysis of electrophoretically heterogeneous lipopolysaccharides of Escherichia coli by immunoblotting. FEMS Microbiology Letters 22:193–196
    [Google Scholar]
  23. Leitch R. A., & Richards J. C. 1988; Structure of the O-chain of the lipopolysaccharide of Pasteurella haemolytica serotype T3. Biochem-istry and Cell Biology 66:1055–1065
    [Google Scholar]
  24. Lugtenberg B., , Boxtel R., & Dejong M. 1984; Atrophic rhinitis in swine: correlation of Pasteurella multocida pathogenicity with membrane proteins and lipopolysaccharide patterns. Infection and Immunity 46:48–54
    [Google Scholar]
  25. Mandrell R., , Schneider H., , Apicella M., , Zollinger W., , Rice P.A., & Griffiss J. M. 1986; Antigenic and physical diversity of Neisseria gonorrhoeae lipooligosaccharides. Infection and Immunity 54:63–69
    [Google Scholar]
  26. Manning P. J., , Naasz M.A., , DEIONG D., & LEARY S. L. 1986; Pasteurellosis in laboratory rabbits: characterisation of lipopolysac-charides of Pasteurella multocida by polyacrylamide gel electrophore-sis, immunoblot technique, and enzyme-linked immunosorbant assay. Infection and Immunity 53:460–463
    [Google Scholar]
  27. Ørskov F., & Ørskov I. 1978; Serotyping of Enterobacteriaceae, with special emphasis on K antigen determination. Methods in Microbiology 11:1–77
    [Google Scholar]
  28. Ørskov F., & Ørskov I. 1984; Serotyping of Escherichia coli . Methods in Microbiology 14:43–112
    [Google Scholar]
  29. Peppler M. S. 1984; Two physically and serologically distinct lipopolysaccharide profiles in strains of Bordetella pertussis and their phenotype variants. Infection and Immunity 43:224–232
    [Google Scholar]
  30. Perry M. B., & Babiuk L. A. 1984; Structure of polysaccharide chain of Pasteurella haemolytica (serotype 4) lipopolysaccharide. Canadian Journal of Biochemistry and Cell Biology 62:108–114
    [Google Scholar]
  31. Preston M. A., & Penner J. L. 1987; Structural and antigenic properties of lipopolysaccharides from serotype reference strains of Campylobacter Jejuni . Infection and Immunity 55:1806–1812
    [Google Scholar]
  32. Richards J. C., & Leitch R. A. 1989; Elucidation of the structure of the Pasteurella haemolytica serotype TIO lipopolysaccharide O-antigen by N. M. R. spectroscopy. Carbohydrate Research 186:275–286
    [Google Scholar]
  33. Rimler R. B. 1990; Comparison of Pasteurella multocida lipopolysac-charides by sodium dodecyl sulfate-polyacrylamide gel electrophore-sis to determine relation between group B and E hemorrhagic septicemia strains and serologically related group A strains. Journal of Clinical Microbiology 28:654–659
    [Google Scholar]
  34. Rimsay R. L., , Coyle-Dennis J.E., , Lauerman L. H., & Squire P. G. 1981; Purification and biological characterization of endotoxin fractions from Pasteurella haemolytica . American Journal of Veterin-ary Research 42:2134–2138
    [Google Scholar]
  35. Rivera M., , Bryan L. E., , Hancock R.E.W., & Mcgroarty E. J. 1988; Heterogeneity of lipopolysaccharide from Pseudomonas aeruginosa. Analysis of lipopolysaccharide chain length by gel filtration and sodium dodecyl sulfate-polyacrylamide gel electro-phoresis.. Journal of Bacteriology 170:512–521
    [Google Scholar]
  36. Shreeve B. J., , Biberstein E. L., & Thompson D. A. 1972; Variation in carrier rates of Pasteurella haemolytica in sheep. II. Diseased flocks. Journal of Comparative Pathology 82:111–118
    [Google Scholar]
  37. Sturm S., , Fortnagel P., & Timmis K. N. 1984; immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide. Archives of Microbiology 140:198–201
    [Google Scholar]
  38. Towbin H., , Staehelin T., & Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
  39. Tsai C.-M., & Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
  40. Tsai C. M., , Boykins R., & Frasch C. E. 1983; Heterogeneity and variation among Neisseria meningitidis lipopolysaccharide. Journal of Bacteriology 155:498–504
    [Google Scholar]
  41. Tsai C.-M., , Mocca L. F., & Frasch C. E. 1987; lmMunotype epitopes of Neisseria meningitidis lipooligosaccharide types 1 through 8. Infection and Immunity 55:1652–1656
    [Google Scholar]
  42. Utley S. R., , Bhat U. R., , Byrd W., & Kadis S. 1992; Characterization of lipopolysaccharides from four Pasteurella haemolytica serotype strains : evidence for presence of sialic acid in serotypes l and 5. FEMS Microbiology Letters 92:211–216
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-10-2185
Loading
/content/journal/micro/10.1099/00221287-138-10-2185
Loading

Data & Media loading...

Most cited Most Cited RSS feed