1887

Abstract

In , iron assimilation by means of the siderophore enterobactin requires two hydrophobic cytoplasmic membrane proteins, FepD and FepG, which are essential components of a binding-protein-dependent transport system. Such components are typically difficult to detect. Here we report observation of the and gene products in polyacrylamide gels; they appeared as diffuse bands at positions consistent with smaller sizes than those predicted by sequence analysis. Translational coupling was suggested by the lack of a detectable product from the message in the absence of translation of the upstream message. The orientation of FepD/FepG in the membrane was predicted based on their similarities in sequence and hydrophobicity to FhuB.

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1992-10-01
2024-04-19
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References

  1. Ames G. F.-L., , Mimura C. S., & Shyamala V. 1990; Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases. FEMS Microbiology Reviews 75:429–446
    [Google Scholar]
  2. Bagg A., & Neilands J. B. 1987; Molecular mechanism of regulation of siderophore-mediated iron asimilation. Microbiological Reviews 51:509–518
    [Google Scholar]
  3. Beyreuther K., , Bieseler B., , Ehring R., , Griesser H.-W., , Mieschendah M., , Müller-Hill B., & Triesch I. 1980; Investigation of structure and function of lactose permease of Escherichia coli . Biochemical Society Transactions 8:675–676
    [Google Scholar]
  4. Chenault S. S., & Earhart C. F. 1991; Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease. Molecular Microbiology 5:1405–1413
    [Google Scholar]
  5. Dassa E., & Hofnung M. 1985; Sequence of gene malG in E. coli K12: homologies between integral membrane components from binding protein-dependent transport systems. EMBO Journal 4:2287–2293
    [Google Scholar]
  6. Earhart C. F. 1987 Ferrienterobactin transport in Escherichia coli . In Iron Transport in Microbes, Plants and Animals, pp. 67–84 Edited by Winkelman G., , van der Helm D., & person-group-type="editor"> Neilands J.B. Weinheim: VCH;
    [Google Scholar]
  7. Elkins M. F., & Earhart C. F. 1989; Nucleotide sequence and regulation of Escherichia coli gene for ferrienterobactin transport protein FepB. Journal of Bacteriology 171:5443–5451
    [Google Scholar]
  8. Gold L. 1988; Posttranscriptional regulatory mechanisms in Escherichia coli . Annual Review of Biochemistry 57:199–233
    [Google Scholar]
  9. Hall M. N., , Gabay J., , Debarbouille M., & Schwartz M. 1982; A role for mRNA secondary structure in the control of translation initiation. Nature, London 295:616–618
    [Google Scholar]
  10. Hantke K. 1981; Regulation of ferric iron transport in Escherichia coli K 12: isolation of a constitutive mutant. Molecular and General Genetics 182:288–292
    [Google Scholar]
  11. Higgins C. F., , Hyde S. C., , Minnack M. M., , Gileadi U., , Gill D. R., & Gallagher M. P. 1990a; Binding-protein-dependent transport systems. Journal of Bioenergetics and Biomembranes 22:571–592
    [Google Scholar]
  12. Higgins C. F., , Gallagher M. P., , Hyde; S. C., , Mimmack· M. L., & Pearce S. R. 1990b; Periplasmic binding protein-dependent transport systems: the membrane-associated components. Philosophical Transactions of the Royal Society of London B 326:353–365 Ito K. 1984; Identification of the secY (prlA) gene product involved in protein export in Escherichia coli . Molecular and General Genetics 197:204–208
    [Google Scholar]
  13. Kerppola R. E., , Shyamala V. K., , Klebba P., & Ames G. F.-L. 1991; The membrane-bound proteins of periplasmic permeases form a complex. Identification of the histidine permease HisQMP complex. Journal of Biological Chemistry 264:9857–9865
    [Google Scholar]
  14. Koster W., & Braun V. 1986; Iron hydroxamate transport of Escherichia coli: nucleotide sequence of the fhuB gene and identification of the protein. Molecular and General Genetics 204:435–442
    [Google Scholar]
  15. Koster W., & Braun V. 1989; Iron-hydroxamate transport into Escherichia coli K12: localization of FhuD in the periplasm and of FhuB in the cytoplasmic membrane. Molecular and General Genetics 217:233–239
    [Google Scholar]
  16. Nagel De Zwaig, R., & Luria S. E. 1967; Genetics and physiology of colicin-tolerant mutants of Escherichia coli . Journal of Bacteriology 94:1112–1123
    [Google Scholar]
  17. Neilands J. B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  18. Ozenberger B. A., , Nahlik M. S., & Mcintosh M. A. 1987; Genetic organization of multiple fep genes encoding ferric entero-bactin transport functions in Escherichia coli . Journal of Bacteriology 169:3638–3646
    [Google Scholar]
  19. Pearce S. R., , Mimmack M. L., , Gallagher M. P., , Gileadi U., , Hyde S. C., & Higgins C. F. 1992; Membrane topology of the integral membrane components, OppB and OppC, of the oligopep-tide permease of Salmonella typhimurium . Molecular Microbiology 6:47–57
    [Google Scholar]
  20. Pierce J. R., & Earhart C. F. 1986; Escherichia coli K12 envelope proteins specifically required for ferrienterobactin uptake. Journal of Bacteriology 166:930–936
    [Google Scholar]
  21. Pierce J. R., , Pickett C. L., & Earhart C. F. 1983; Twofep genes are required for ferrienterochelin uptake in Escherichia coli K-12. Journal of Bacteriology 155:330–336
    [Google Scholar]
  22. Postle K. 1990; TonB and the Gram-negative dilemma. Molecular Microbiology 4:2019–2025
    [Google Scholar]
  23. Shea C. M., & Mcintosh M. A. 1991; Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli . Molecular Microbiology 5:1415–1428
    [Google Scholar]
  24. Staudenmaier H., , Van Hove B., , Yaraghi A., & Braun V. 1989; Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli . Journal of Bacteriology 171:2626–2633
    [Google Scholar]
  25. Tabor S., & Richardson C. C. 1985; A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proceedings of the National Academy of Sciences of the United States of America 821074–1078
    [Google Scholar]
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