1887

Abstract

pCI528 is a 46 kb plasmid, originally isolated from subsp. UC503, which causes a decrease in bacteriophage adsorption in lactococcal strains. Cell surface characteristics of two pCI528-containing strains, subsp. UC503 and subsp. UC505, were compared with those of their isogenic pCI528-cured derivatives, UC563 and MG1363, respectively. Following centrifugation, strains containing pCI528 produced a loose fluffy pellet. The presence of pCI528 also caused a ‘hard’ colony morphology on agar media. The fluffy pellet effect mediated by pCI528 could be eliminated by washing cells in 0.05 M-NaOH, which also restored full sensitivity to phage in the subsp. UC505 background. GLC analysis of cell wall material indicated that pCI528-containing hosts had significantly elevated levels of both galactose and rhamnose. Marked alterations in the cell surface of strains harbouring pCI528 were observed in electron micrographs.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-10-2137
1992-10-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/10/mic-138-10-2137.html?itemId=/content/journal/micro/10.1099/00221287-138-10-2137&mimeType=html&fmt=ahah

References

  1. Chopin A., , Chopin M. C., , Moillo-Bait A., & Langella P. 1984; Two plasmid determined restriction and modification systems in Streptococcus lactis . Plasmid 11:260–263
    [Google Scholar]
  2. Coffey A., , Fitzgerald G., & Daly C. 1989; Identification and characterisation of a plasmid encoding abortive infection from Lactococcus lactis ssp. lactis UC811. Netherlands Milk and Dairy Journal 43:229–244
    [Google Scholar]
  3. Coffey A., , Costello V., , Daly C., & Fitzgerald G. 1991 Plasmid encoded bacteriophage insensitivity in members of the genus Lactococcus, with special reference to pCI829. In Genetics and Molecular Biology of Streptococci, Lactococci and Enterococci, pp. 131–135 Edited by Dunny G., , Cleary P., & person-group-type="editor"> Mckay L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Cogan M. T. M., , Peiterson N., & Sellars R. L. 1991; Starter systems. Bulletin of the International Dairy Federation 263:16–23
    [Google Scholar]
  5. Costello V. 1988 Characterization of bacteriophage-host interaction in Streptococcus cremoris UC503 and related lactic streptococci. PhD thesis National University of Ireland:
    [Google Scholar]
  6. Daly C., & Fitzgerald G. 1987 Mechanisms of bacteriophage insensitivity in the lactic streptococci. In Streptococcal Genetics, pp. 259–268 Edited by Ferretti J. J., & person-group-type="editor"> Curtis R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Everson T. C. 1991; Control of phage in the dairy plant. Bulletin of the International Dairy Federation 263:24–28
    [Google Scholar]
  8. Gasson M. J. 1983; Plasmid complements of Streptococcus lactis NCDO712 and other streptococci after protoplast-induced curing. Journal of Bacteriology 154:1–9
    [Google Scholar]
  9. Hill C., , Pierce K., & Klaenhammer T. R. 1989; The conjugative plasmid pTR2030 encodes two bacteriophage defence mech-anisms in lactococci, restriction modification (R/M+) and abortive infection (Hsp+). Applied and Environmental Microbiology 55:2416–2419
    [Google Scholar]
  10. Ishiguro E. E., , Kay W. W., , Ainsworth T., , Chamberlain J. B., , Austen R. A., , Buckley J. T., & Trust T. J. 1981; Loss of virulence during culture of Aeromonas salmonicida at high temperature. Journal of Bacteriology 148:333–340
    [Google Scholar]
  11. Klaenhammer T. R. 1989; Genetic characterisation of multiple mechanisms of phage defense from a prototype phage-insensitive strain, Lactococcus lactis ME2. Journal of Dairy Science 72:3429–3443
    [Google Scholar]
  12. Lawrence R. C. 1978; Action of bacteriophage on lactic acid bacteria: consequences and protection. New Zealand Journal of Dairy Science and Technology 13:129–136
    [Google Scholar]
  13. Rosenberg M., , Gutnick D., & Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  14. Sanders M. E. 1988; Phage resistance in lactic acid bacteria. Biochimie 70:411–422
    [Google Scholar]
  15. Sanders M. E., & Klaenhammer T. R. 1981; Evidence for plasmid linkage of restriction and modification in Streptococcus cremoris KH. Applied and Environmental Microbiology 42:944–950
    [Google Scholar]
  16. Sanders M. E., & Klaenhammer T. R. 1983; Characterisation of phage sensitive mutants of a phage insensitive strain of Streptococcus lactis: evidence for a plasmid determinant that prevents phage adsorption. Applied and Environmental Microbiology 46:1125–1133
    [Google Scholar]
  17. Sijtsma L., , Sterkenberg A., & Wouters J. T. M. 1988; Properties of the cell walls of Lactococcus lactis ssp. cremoris SK110 and SK112 and their relationship to bacteriophage resistance. Applied and Environmental Microbiology 54:2808–2811
    [Google Scholar]
  18. Sijtsma L., , Jansen N., , Hazeleger W. C., , Wouters J. T. M., & Hellingwerf K. J. 1990a; Cell surface characteristics of bacteriophage resistant Lactococcus lactis ssp. cremoris SK110 and its bacteriophage sensitive variant SK112. Applied and Environmental Microbiology 56:3230–3233
    [Google Scholar]
  19. Sijtsma L., , Wouters J. T. M., & Hellingwerf K. J. 1990b; Isolation and characterisation of lipoteichoic acid, a cell envelope component involved in preventing phage adsorption from Lactococcus lactis ssp cremoris SK110. Journal of Bacteriology 172:7126–7130
    [Google Scholar]
  20. Terzaghi B. E., & Sandine W. E. 1975; Improved medium for lactic streptococci and their bacteriophages. Applied Microbiology 29:807–813
    [Google Scholar]
  21. Tortorello M. L., , Chang P. K.., , Ledford R. A, & Dunny G. M. 1990; Plasmid associated antigens associated with resistance to phage adsorption in Lactococcus lactis . In Abstracts of the 3rd International ASM Conference on Streptococcal Genetics, A/50
    [Google Scholar]
  22. Valyasevi R., , Sandine W. E., & Geller B. L. 1990; The bacteriophage kb receptor of Lactococcus lactis ssp. cremoris KH is the rhamnose of the extracellular wall polysaccharide. Applied and Environmental Microbiology 56:1882–1889
    [Google Scholar]
  23. Vedamuthu E. R., & Neville J. M. 1986; Involvement of a plasmid in production of ropiness (mucoidness) in milk cultures by Streptococcus cremoris MS. Applied and Environmental Microbiology 51:677–682
    [Google Scholar]
  24. Devos W. M., , Underwood H. M., & Davies F. L. 1984; Plasmid encoded bacteriophage resistance in Streptococcus cremoris SK11. FEMS Microbiology Letters 23:175–178
    [Google Scholar]
  25. Wallace R. J. 1980; Cytoplasmic reserve polysaccharide of Selenomonas ruminantium . Applied and Environmental Microbiology 39:630–634
    [Google Scholar]
  26. Watanabe K., , Ishibashi K., , Iki K., , Nakashima Y., , Hayashida M., & Amako K. 1987; Cell surface characteristics of some phage-resistant strains of Lactobacillus casei . Journal of Applied Bacteriology 63:197–200
    [Google Scholar]
  27. Wu T. C., & Park J. T. 1971; Chemical characterisation of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus . Journal of Bacteriology 108:874–884
    [Google Scholar]
/content/journal/micro/10.1099/00221287-138-10-2137
Loading
/content/journal/micro/10.1099/00221287-138-10-2137
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error