RT Journal Article SR Electronic(1) A1 Cabib, Enrico A1 Silverman, Sanford J. A1 Shaw, J. AndrewYR 1992 T1 Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Saccharomyces cerevisiae JF Microbiology, VO 138 IS 1 SP 97 OP 102 DO https://doi.org/10.1099/00221287-138-1-97 PB Microbiology Society, SN 1465-2080, AB Summary: Previous results [E. Cabib, A. Sburlati, B. Bowers & S. J. Silverman (1989) Journal of Cell Biology 108, 1665-1672] strongly suggested that the lysis observed in daughter cells of Saccharomyces cerevisiae defective in chitin synthase 1 (Chs1) was caused by a chitinase that partially degrades the chitin septum in the process of cell separation. Consequently, it was proposed that in wild-type cells, Chs1 acts as a repair enzyme by replenishing chitin during cytokinesis. The chitinase requirement for lysis has been confirmed in two different ways: (a) demethylallosamidin, a more powerful chitinase inhibitor than the previously used allosamidin, is also a much better protector against lysis and (b) disruption of the chitinase gene in chs1 cells eliminates lysis. Reintroduction of a normal chitinase gene, by transformation of those cells with a suitable plasmid, restores lysis. The percentage of lysed cells in strains lacking Chs1 was not increased by elevating the chitinase level with high-copy-number plasmids carrying the hydrolase gene. Furthermore, the degree of lysis varied in different chs1 strains; lysis was abolished in chs1 mutants containing the scs1 suppressor. These results indicate that, in addition to chitinase, lysis requires other gene products that may become limiting., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-138-1-97