1887

Abstract

Summary: Previous results [E. Cabib, A. Sburlati, B. Bowers & S. J. Silverman (1989) 108, 1665-1672] strongly suggested that the lysis observed in daughter cells of defective in chitin synthase 1 (Chs1) was caused by a chitinase that partially degrades the chitin septum in the process of cell separation. Consequently, it was proposed that in wild-type cells, Chs1 acts as a repair enzyme by replenishing chitin during cytokinesis. The chitinase requirement for lysis has been confirmed in two different ways: () demethylallosamidin, a more powerful chitinase inhibitor than the previously used allosamidin, is also a much better protector against lysis and () disruption of the chitinase gene in cells eliminates lysis. Reintroduction of a normal chitinase gene, by transformation of those cells with a suitable plasmid, restores lysis. The percentage of lysed cells in strains lacking Chs1 was not increased by elevating the chitinase level with high-copy-number plasmids carrying the hydrolase gene. Furthermore, the degree of lysis varied in different strains; lysis was abolished in mutants containing the suppressor. These results indicate that, in addition to chitinase, lysis requires other gene products that may become limiting.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-1-97
1992-01-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/1/mic-138-1-97.html?itemId=/content/journal/micro/10.1099/00221287-138-1-97&mimeType=html&fmt=ahah

References

  1. Bulawa C. E., Slater M., Cabib E., Au-Young J., Sburlati A., Adair W. L. JR, Robbins P. W. 1986; The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo . Cell 46:213–225
    [Google Scholar]
  2. Bulawa C. E., Osmond B. C. 1990; Chitin synthase I and chitin synthase II are not required for chitin synthesis in vivo in Saccharomyces cerevisiae . Proceedings of the National Academy of Sciences of the United States of America 877424–7428
    [Google Scholar]
  3. Cabib E. 1987; The synthesis and degradation of chitin. Advances in Enzymology 59:59–101
    [Google Scholar]
  4. Cabib E., Sburlati A., Bowers B., Silverman S.J. 1989; Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae . Journal of Cell Biology 108:1667–1672
    [Google Scholar]
  5. Correa J. U., Elango N., Polacheck I., Cabib E. 1982; Endochitinase, a mannan-associated enzyme from Saccharomyces cerevisiae . Journal of Biological Chemistry 257:1392–1397
    [Google Scholar]
  6. Elango N., Correa J. U., Cabib E. 1982; Secretory character of yeast chitinase. Journal of Biological Chemistry 257:1398–1400
    [Google Scholar]
  7. Fernandez M. P., Correa J. U., Cabib E. 1982; Activation of chitin synthetase in permeabilized cells of a Saccharomyces cerevisiae mutant lacking proteinase B. Journal of Bacteriology 152:1255–1264
    [Google Scholar]
  8. Ito H., Fukuda Y., Murata K., Kimura A. 1983; Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology 153:163–168
    [Google Scholar]
  9. Kang M S, Elango N., Mattia E., Au-Young J., Robbins P. W., Cabib E. 1984; Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product. Journal of Biological Chemistry 259:14966–14972
    [Google Scholar]
  10. Kuranda M. J., Robbins P. W. 1987; Cloning and heterologous expression of glycosidase genes from Saccharomyces cerevisiae . Proceedings of the National Academy of Sciences of the United States of America 842585–2589
    [Google Scholar]
  11. Kuranda M. J., Robbins P. W. 1991; Chitinase is required for cell separation during growth of Saccharomyces cerevisiae . Journal of Biological Chemistry 266:19758–19767
    [Google Scholar]
  12. Orlean P. 1987; Two chitin synthases inSaccharomyces cerevisiae . Journal of Biological Chemistry 262:5732–5739
    [Google Scholar]
  13. Roberts R. L., Bowers B., Slater M. L., Cabib E. 1983; Chitin synthesis and localization in cell division cycle mutants of Saccharomyces cerevisiae . Molecular and Cellular Biology 3:922–930
    [Google Scholar]
  14. Sakuda S., Nishimoto Y., Ohi M., Watanabe M., Takayama S., Isogai A., Yamada Y. 1990; Effect of demethylallosamidin, a potent yeast chitinase inhibitor, on the cell division of yeast. Agricultural and Biological Chemistry 54:1333–1335
    [Google Scholar]
  15. Sburlati A., Cabib E. 1986; Chitin synthethase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae . Journal of Biological Chemistry 261:15147–15152
    [Google Scholar]
  16. Shaw J. A., Mol P. C., Bowers B., Silverman S. J., Valdivieso Μ. H., Duran A., Cabib E. 1991; The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle . Journal of Cell Biology 114:111–123
    [Google Scholar]
  17. Sherman F., Fink G. R., Hicks J. B. 1986 Methods in Yeast Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Silverman S. J., Sburlati A., Slater M. L., Cabib E. 1988; Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae . Proceedings of the National Academy of Sciences of the United States of America 854735–4739
    [Google Scholar]
  19. Silverman S. J., Shaw J. A., Cabib E. 1991; Proteinase B is, indeed not required for chitin synthetase 1 function in Saccharomyces cerevisiae . Biochemical and Biophysical Research Communciations 174:204–210
    [Google Scholar]
/content/journal/micro/10.1099/00221287-138-1-97
Loading
/content/journal/micro/10.1099/00221287-138-1-97
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error