
Full text loading...
Summary: Methyl mercaptan (MM)-oxidase was purified to near homogeneity from Thiobacillus thioparus TK-m grown on dimethyl sulphide. The enzyme was a monomer with an M r value of approximately 40000. It oxidized MM stoichiometrically to formaldehyde, S2- and H2O2. The enzyme had a K m of 31·3 μM for MM. It was also shown to oxidize ethyl mercaptan. MM-oxidase from T. thioparus was shown to have some different characteristics from MM-oxidase of Hyphomicrobium EG, such as a higher K m, the absence of inhibition by S2- and the inability to catalyse the oxidation of S2-.
Article metrics loading...
Full text loading...
References
Data & Media loading...