DNA homology between siderophore genes from fluorescent pseudomonads Free

Abstract

Summary: Many species of pseudomonads produce fluorescent siderophores involved in iron uptake. We have investigated the DNA homology between the siderophore synthesis genes of an opportunist animal pathogen, , and three plant-associated species and sp. B10. There is extensive homology between the DNA from the different species, consistent with the suggestion that the different siderophore synthesis genes have evolved from the same ancestral set of genes. The existence of DNA homology allowed us to clone some of the siderophore synthesis genes from , and genetic mapping indicates that the cloned DNA lies in a locus previously identified as being involved in siderophore production.

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1992-01-01
2024-03-29
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References

  1. Ankenbauer R., Hanne L., Cox C. D. 1986; Mapping of mutations in Pseudomonas aeruginosadefective in pyoverdin production. Journal of Bacteriology 167:7–11
    [Google Scholar]
  2. Bodey G. P., Bolivar R., Fainstein V., Jadeja L. 1983; Infections caused by Pseudomonas aeruginosa . Reviews of Infectious Diseases 5:279–307
    [Google Scholar]
  3. Dagert M., Ehrlich S. D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia colicells. Gene 6:23–28
    [Google Scholar]
  4. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  5. Frischauf A.-M. 1987; Digestion of DNA: size fractionation. Methods in Enzymology 152:183–189
    [Google Scholar]
  6. Frischauf A.-M., Lehrach H., Poustka A., Murray N. 1983; Lambda replacement vectors carrying polylinker sequences. Journal of Molecular Biology 170:827–842
    [Google Scholar]
  7. Grossberger D. 1987; Minipreps of DNA from bacteriophage lambda. Nucleic Acids Research 15: 6737
    [Google Scholar]
  8. Haas D., Holloway B,. W., Schambock A., Leisinger T. 1977; The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa . Molecular and General Genetics 154:7–22
    [Google Scholar]
  9. Hohnadel D., Haas D., Meyer J.-M. 1986; Mapping of mutations affecting pyoverdine production in Pseudomonas aeruginosa . FEMS Microbiology Letters 36:195–199
    [Google Scholar]
  10. Hohnadel D., Meyer J.-M. 1988; Specificity of pyoverdine- mediated iron uptake among fluorescent Pseudomonasstrains. Journal of Bacteriology 170:4865–4873
    [Google Scholar]
  11. Holloway B. W. 1965; Variations in restriction and modification of bacteriophage following increase of growth temperature of Pseudomonas aeruginosa . Virology 25:634–642
    [Google Scholar]
  12. Holloway, B. W, Zhang C. 1990 Pseudomonas aeruginosaPAO. In Genetic Maps. Locus Maps of Complex Genomes, 5th edn. pp 2.71–2.78 Edited by O’Brien S. J. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid vector cloning. Nucleic Acids Research 9:2989–2999
    [Google Scholar]
  14. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine 44:301–307
    [Google Scholar]
  15. Kloepper J. W., Leong J., Teintze, M, Schroth M. N. 1980; Enhanced plant growth by siderophores produced by plant growthpromoting bacteria. Nature, London 286:885–886
    [Google Scholar]
  16. Lawson E. C., Jonsson C.B., Hemming B. C. 1986 Genotypic diversity of fluorescent pseudomonads as revealed by Southern hybridization analysis with siderophore-related gene probes. In Iron, Siderophores and Plant Diseases pp. 315–329 Edited by Swinburne T. R. New York: Plenum Press;
    [Google Scholar]
  17. Loper J. E., Orser C. S, Panopoulos N. J., Schroth M. N. 1984; Genetic analysis of fluorescent pigment production in Pseudomonas syringaepv syringae . Journal of General Microbiology 130:1507–1515
    [Google Scholar]
  18. Magazin M.D., Moores M.C, Leong J. 1986; Cloning of the gene coding for the outer membrane receptor protein for ferric pseudobactin, a siderophore from a plant growth-promoting Pseudomonasstrain. Journal of Biological Chemistry 261:795–799
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  21. Marugg J. D., van Spanje M., Hoekstra W. P. M., Schippers B., Weisbeek P. J. 1985; Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putidaWCS358. Journal of Bacteriology 164:563–570
    [Google Scholar]
  22. Marugg J. D., DeWeger L. A., Neilander H. B., Oorthuizen M., Recourt K., Lugtenberg B., van der Hofstad G. A. J. M., Wiesbeek P. J. 1989; Cloning and characterization of a gene encoding an outer membrane protein required for siderophore- mediated uptake of Fe3+in Pseudomonas putidaWCS358. Journal of Bacteriology 171:2819–2826
    [Google Scholar]
  23. Matsumoto H, Nakazawa T., Ohta S, Terawaki Y. 1981; Chromosomal locations of cat A, pobA, pcaA, dcu chugenes in Pseudomonas aeruginosa . Genetical Research 38:251–266
    [Google Scholar]
  24. Meyer, J. M., Abdallah M. A. 1978; The fluorescent pigment of Pseudomonas fluorescens. biosynthesis, purification and physicochemical properties. Journal of General Microbiology 107:319–328
    [Google Scholar]
  25. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Moores J. C., Magazin M., Ditta G. S., Leong J. 1984; Cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting Pseudomonasstrain. Journal of Bacteriology 157:53–58
    [Google Scholar]
  27. O’Hoy K., Krishnapillai V. 1987; Recalibration of the Pseudomonas aeruginosastrain PAO chromosome map in time units using high-frequency-of-recombination donors. Genetics 115:611–618
    [Google Scholar]
  28. O’Sullivan D. J., Morris J., O’Gara F. 1990; Identification of an additional ferric-siderophore uptake gene clustered with receptor, biosynthesis and /ur-like regulatory genes in fluorescent Pseudomonassp. strain Ml 14. Applied and Environmental Microbiology 56:2056–2064
    [Google Scholar]
  29. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivogenetic engineering: transposon mutagenesis in Gram negative bacteria. Biotechnology 1:784–791
    [Google Scholar]
  30. Simon R., O’Connell M., Labes M, Puhler A. 1986; Plasmid vectors for the genetic analysis and manipulation of Rhizobia and other gram-negative bacteria. Methods in Enzymology 118:640–659
    [Google Scholar]
  31. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7- derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  32. Willetts N. 1984; Conjugation. Methods in Microbiology 17:33–59
    [Google Scholar]
  33. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the MBmp 18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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