Physical mapping of the region of an Australian methicillin-resistant lineage and a closely related American strain Free

Abstract

Summary: Methicillin-resistant (Mc) staphylococci contain chromosomal DNA that is absent from Mc cells. This extra DNA harbours the methicillin resistance determinant and often other resistance determinants. The region can differ substantially in structure among different isolates. We present studies on the region of a group of isolates prevalent in Australia and London. Southern hybridization analyses of a prototype Australian isolate, ANS46, and an isogenic Mc deletion mutant, ANS62, allowed the physical map of the region to be extended to 55 kb. The DNA corresponding to the deletion, which includes and resistance determinants for mercury, cadmium (Cd) and tetracycline, amounted to 41 kb. It was bounded precisely at one end by the macrolides-lincosamides-streptogramin B (MLS)-resistance transposon, Tn. Near the other end was an element with homology to Tn, ΨTn, which carried the Cd determinant. The region of an American Mc isolate, R35, was found to be virtually the same as that of ANS46, except that it lacked Tn. Another class of American Mc isolates, prevalent since 1987, differs markedly from ANS46 in region organization. However, this other American class also contains an insertion of Tn in the region, and the attachment site for this insertion was found to have significant homology to attachment sites for the Tn and ΨTn insertions in the region of the Australian strain. These results suggest possible roles of Tn and Tn-like elements in the evolutionary variation of the region.

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1992-01-01
2024-03-28
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References

  1. BARRY A. L., THORNSBERRY C. 1980 Susceptibility testing: diffusion test procedures. In Manual of Clinical Microbiology, 3rd edn. pp 463–474 Edited by Lennette E. A. Washington: American Society for Microbiology;
    [Google Scholar]
  2. BECK W. D., BERGER-BACHI B., KAYSER F. H. 1986; Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of wec-specific DNA. Journal of Bacteriology 165:373–378
    [Google Scholar]
  3. BERENT S. L., MAHMOUDI M., TORCZYNSKI R. M., BRAGG P. W., BOLLON A. P. 1985; Comparison of oligonucleotide and long DNA fragments as probes in DNA and RNA dot, Southern, Northern, colony and plaque hybridization. Biotechniques 3:208–220
    [Google Scholar]
  4. CHIKRAMANE S. G., MATTHEWS P. R., NOBLE W. C., STEWART P. R., DUBIN D. T. 1991; Tn554 inserts in methicillin-resistant Staphylococcus aureus from Australia and England: comparison with an American methicillin-resistant group. Journal of General Microbiology 137:1303–1311
    [Google Scholar]
  5. COOKSON B. D., PHILLIPS I. 1988; Epidemic methicillin-resistant Staphylococcus aureus . Journal of Antimicrobial Chemotherapy 21:suppl. C57–65
    [Google Scholar]
  6. DUBIN D. T. 1990 Tn554 and related DNA: mapping and tracking the staphylococcal chromosome. In Molecular Biology of the Staphylococci . pp 85–98 Edited by Novick R. New York: VCH Publishing;
    [Google Scholar]
  7. DUBIN D. T., MATTHEWS P. R., CHIKRAMANE S. G., STEWART P. R. 1991; Physical mapping of DNA from the mec region of an American methicillin Staphylococcus aureus lineage. Antimicrobial Agents and Chemotherapy 35:1661–1665
    [Google Scholar]
  8. INGLIS B., MATTHEWS P. R., STEWART P. R. 1990; Induced deletions within a cluster of resistance genes in the mec region of the chromosome of Staphylococcus aureus . Journal of General Micro-biology 136:2231–2239
    [Google Scholar]
  9. KHAN S. A., NOVICK P. R. 1983; Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus . Plasmid 10:251–256
    [Google Scholar]
  10. LADDAGA R. A., CHU L., MISRA T. K., SILVER S. 1987; Nucleotide sequence and expression of the mercurial resistance operon from Staphylococcus aureus pl 528. Proceedings of the National Academy of Sciences of the United States of America 84:5106–5110
    [Google Scholar]
  11. LYON B. R., SKURRAY R. 1987; Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiological Reviews 51:88–134
    [Google Scholar]
  12. MATTHEWS P. R., REED K. C., STEWART P. R. 1987; The cloning of chromosomal DNA associated with methicillin and other resistances in Staphylococcus aureus . Journal of General Microbiology 133:1919–1929
    [Google Scholar]
  13. MATTHEWS P. R., INGLIS B., STEWART P. R. 1990 Clustering of resistance genes in the mec region of the chromosome of Staphylococcus aureus . In Molecular Biology of the Staphylococci pp 59–83 Edited by Novick R. New York: VCH Publishing;
    [Google Scholar]
  14. MURPHY E. 1990 Properties of the site-specific transposable element Tn554 . In Molecular Biology of the Staphylococci pp 123–136 Edited by Novick R. New York: VCH Publishing;
    [Google Scholar]
  15. MURPHY E., HUWYLER L., BASTOS M. C. 1985; Transposon Tn554: complete nucleotide sequence and isolation of tranposition-defective and antibiotic-sensitive mutants. EMBO Journal 4:3357–3365
    [Google Scholar]
  16. MURPHY E., PHILLIPS S., EDELMAN I., NOVICK R. P. 1981; Tn554: isolation and characterization of plasmid insertions. Plasmid 5:292–305
    [Google Scholar]
  17. PAVILLARD R., HARVEY K., DOUGLAS D., HEWSTONE A., ANDREW J., COLLOPY B., ASCHE V., CARSON P., DAVIDSON A., GILBERT G., SPICER J., TOSOLINI F. 1982; Epidemic of hospital-acquired infection due to methicillin-resistant Staphylococcus aureus in major Victorian hospitals. Medical Journal of Australia 1:451–454
    [Google Scholar]
  18. PROJAN S. J., KORNBLUM K., MOGHAZEK S. L., EDELMAN I., GENNARO M. L., NOVICK R. P. 1985; Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus . Molecular and General Genetics 199:452–464
    [Google Scholar]
  19. SANGER F., NICKLEN S., COULSON A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  20. SKINNER S., INGLIS B., MATTHEWS P. R., STEWART P. R. 1988; Mercury and tetracycline resistance genes and flanking repeats associated with methicillin resistance on the chromosome of Staphylococcus aureus . Molecular Microbiology 2:289–298
    [Google Scholar]
  21. SONG M. D., WACHI M., DOI M., ISHINO F., MATSUHASHI M. 1987; Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Letters 221:167–171
    [Google Scholar]
  22. THAKKER-VARIA S., JENSSEN W. D., MOON-MCDERMOTT L., WEIN-STEIN M. P., DUBIN D. T. 1987; Molecular epidemiology of macrolides-lincosamides-streptogramin B resistance in Staphylococcus aureus and coagulase-negative staphylococci. Antimicrobial Agents and Chemotherapy 31:735–743
    [Google Scholar]
  23. TILLOTSON L. E., JENSSEN W. D., MOON-MCDERMOTT L., DUBIN D. T. 1989; Characterization of a novel insertion of the macrolides- lincosamides-streptogramin B resistance transposon Tn554 in methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis . Antimicrobial Agents and Chemotherapy 33:541–550
    [Google Scholar]
  24. UBUKATA K., NONOGUCHI R., MATSUHASHI M., DONG SONG M., KONNO M. 1989; Restriction maps of the regions coding for the methicillin and tobramycin resistances on chromosomal DNA in methicillin-resistant staphylococci. Antimicrobial Agents and Chemotherapy 33:1624–1626
    [Google Scholar]
  25. WEISS A. A., MURPHY S. D., SILVER S. 1977; Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus . Journal of Bacteriology 132:197–208
    [Google Scholar]
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