Summary: Outer-membrane proteins (OMPs) from type b (strain Eagan), grown both (broth) and (rat intra-peritoneal), were separated by SDS-PAGE. The major OMPs were present in both growth conditions although the amounts of OMP a and OMP d were reduced in rat-grown organisms. There were strong additional bands in -grown organisms at 51 and 92 kDa. Antiserum was raised in rabbits against -grown bacteria, and absorbed with lysates of -grown bacteria. This serum was used in Western blot analysis of OMPs from - and -grown cells to identify immunogenic proteins present in infection. These infection-associated OMPs had apparent molecular masses of 43 kDa, 48 kDa, 81 kDa and > 200 kDa. Bands of reactivity, of the same molecular mass as some of these, were found on immunoblots when rat and human convalescent sera were used as the source of primary antibody. In particular, a band of 81 kDa was recognized by pooled rat and three human convalescent sera.


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