1887

Abstract

Summary: Outer-membrane proteins (OMPs) from type b (strain Eagan), grown both (broth) and (rat intra-peritoneal), were separated by SDS-PAGE. The major OMPs were present in both growth conditions although the amounts of OMP a and OMP d were reduced in rat-grown organisms. There were strong additional bands in -grown organisms at 51 and 92 kDa. Antiserum was raised in rabbits against -grown bacteria, and absorbed with lysates of -grown bacteria. This serum was used in Western blot analysis of OMPs from - and -grown cells to identify immunogenic proteins present in infection. These infection-associated OMPs had apparent molecular masses of 43 kDa, 48 kDa, 81 kDa and > 200 kDa. Bands of reactivity, of the same molecular mass as some of these, were found on immunoblots when rat and human convalescent sera were used as the source of primary antibody. In particular, a band of 81 kDa was recognized by pooled rat and three human convalescent sera.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-1-155
1992-01-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/1/mic-138-1-155.html?itemId=/content/journal/micro/10.1099/00221287-138-1-155&mimeType=html&fmt=ahah

References

  1. ALLAN I., LOEB M. R., MOXON E. R. 1987; Limited genetic diversity of Haemophilus influenzae (type b). Microbial Pathogenesis 2:139–145
    [Google Scholar]
  2. ANDERSON P., JOHNSTON R. B., SMITH D. H. 1972; Human serum activities against Haemophilus influenzae, type b. Journal of Clinical Investigation 51:31–38
    [Google Scholar]
  3. VAN ALPHEN L., RIEMENS T., POOLMAN J., ZANEN H. C. 1983; Characteristics of major outer membrane proteins of Haemophilus influenzae . Journal of Bacteriology 155:878–885
    [Google Scholar]
  4. VAN ALPHEN L., GEELEN-VAN DEN BROEK L., VAN HAM M. 1990; In vivo and in vitro expression of outer membrane components of Haemophilus influenzae . Microbial Pathogenesis 8:279–288
    [Google Scholar]
  5. BLAKE M.S., JOHNSTON K. H., RUSSELL-JONES G. J., GOTSCHLICH E. C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  6. BRADFORD M. M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  7. BROWN M. R. W., WILLIAMS P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  8. ERWIN A. L., KENNY G. E., SMITH A. L., STULL T. L. 1988; Human antibody response to outer membrane proteins and fimbriae of Haemophilus influenzae type b. Canadian Journal of Microbiology 34:723–729
    [Google Scholar]
  9. GULIG P. A., MCCRACKEN G. H., FRISCH C. F. JR, JOHNSTON K. H., HANSEN E. J. 1982; Antibody response of infants to cell surface- exposed outer membrane proteins of Haemophilus influenzae type b after systemic disease. Infection and Immunity 37:82–88
    [Google Scholar]
  10. HANSEN E. J., FRISCH C. F., MCDADE R. L. JR, JOHNSTON K. H. 1981; Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system. Infection and Immunity 32:1084–1092
    [Google Scholar]
  11. JACOBS A. A. C., DE GRAAF F. K. 1985; Production of K88, K99, and F41 fibrillae in relation to growth phase, and a rapid procedure for adhesin purification. FEMS Microbiology Letters 26:15–19
    [Google Scholar]
  12. JONSON G., SVENNERHOLM A.-M., HOLMGREN J. 1989; Vibrio cholerae expresses cell surface antigens during intestinal infection which are not expressed during in vitro culture. Infection and Immunity 57:1809–1815
    [Google Scholar]
  13. KROLL J. S., MOXON E. R. 1989; Genetic and phenotypic variation in the capsulation and virulence of culture collection strains of Haemophilus influenzae type b. Microbial Pathogenesis 7:449–457
    [Google Scholar]
  14. LOEB M. R., SMITH D. H. 1982; Human antibody response to individual outer membrane proteins of Haemophilus influenzae type b. Infection and Immunity 37:1032–1036
    [Google Scholar]
  15. MILLER V. L., TAYLOR R. K., MEKALANOS J. J. 1987; Cholera toxin transcriptional regulator tox R is a transmembrane DNA binding protein. Cell 48:271–279
    [Google Scholar]
  16. MORTON D. J., WILLIAMS P. 1990; Siderophore-independent acquisition of transferrin-bound iron by Haemophilus influenzae type b. Journal of General Microbiology 136:927–934
    [Google Scholar]
  17. MOULDER J. W. 1985; Comparative biology of intracellular parasitism. Microbiological Reviews 49:298–337
    [Google Scholar]
  18. MOXON E. R. 1990 Haemophilus influenzae. In Principles and Practice of Infectious Diseases1722–1730 Mandell G. L., Douglas R. G. Jr, Bennett J. E. Churchill Livingstone; New York:
    [Google Scholar]
  19. MURPHY J. R., PAPPENHEIMER A. M. JR, TAYART DE BORMS S. 1974; Synthesis of diptheria tox-gene products in Escherichia coli extracts. Proceedings of the National Academy of Sciences of the United States of America 71:11–15
    [Google Scholar]
  20. PIDCOCK K. A., WOOTEN J. A., DALEY B. A., STULL D. A. 1988; Iron acquisition by Haemophilus influenzae . Infection and Immunity 56:721–725
    [Google Scholar]
  21. ROGERS H. J. 1973; Iron-binding catechols and virulence in Escherichia coli . Infection and Immunity 7:445–456
    [Google Scholar]
  22. RUBIN L. G., ZWAHLEN A., MOXON E. R. 1985; Role of intravascular replication in pathogenesis of experimental bacteremia due to Haemophilus influenzae type b. Journal of Infectious Diseases 152:307–314
    [Google Scholar]
  23. SCHNAITMAN C. A. 1971; Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of the isolated cell walls of Escherichia coli . Journal of Bacteriology 108:553–563
    [Google Scholar]
  24. SHAW S., SMITH A. L., ANDERSON P., SMITH D. H. 1974; The paradox of Haemophilus influenzae type b bacteremia in the presence of serum bactercidal activity. Journal of Clinical Investigation 58:1019–1029
    [Google Scholar]
  25. STOSCHECK C. M. 1990; Increased uniformity in the response of the Coomassie blue G protein assay to different proteins. Analytical Biochemistry 184:111–116
    [Google Scholar]
  26. TAYLOR R. K., MILLER V. L., FURLONG D. B., MEKALANOS J. J. 1987; Use of phoA gene fusions to identify a pilus colonisation factor coordinately regulated with cholera toxin. Proceedings of the National Academy of Sciences of the United States of America 84:2833–2837
    [Google Scholar]
  27. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  28. WILLIAMS A. E., MASKELL D. J., MOXON E. R. 1991; Relationship between intracellular survival in macrophages and virulence of Haemophilus influenzae type b. Journal of Infectious Diseases 163:1366–1369
    [Google Scholar]
  29. WILLIAMS P., BROWN M. R. W. 1986; The influence of iron restriction on growth and the expression of outer membrane proteins by Haemophilus influenzae and Haemophilus parainfluenzae . FEMS Microbiology Letters 33:153–157
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-1-155
Loading
/content/journal/micro/10.1099/00221287-138-1-155
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error