1887

Abstract

Summary: The expression of genes complementing arginine auxotrophs of was studied. The activity responsible for the formation of ornithine in was identified as a repressible ornithine acetyltransferase (genetic symbol ) encoded by the same DNA fragment as the and genes. and displayed the same pattern of enzyme activities as . In contrast to previous reports, these organisms consequently use the cyclic pathway of ornithine biosynthesis. also possesses a broad specificity aminoacylase which exhibits low affinity towards -acetyl--ornithine.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-138-1-125
1992-01-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/138/1/mic-138-1-125.html?itemId=/content/journal/micro/10.1099/00221287-138-1-125&mimeType=html&fmt=ahah

References

  1. BROWN K., FINCH P. W., HICKSON I. D., EMMERSON P. 1987; Complete nucleotide sequence of the Escherichia coli argAgene. Nucleic Acids Research 15:10586
    [Google Scholar]
  2. BIRNBOIM H. C., DOLY J. 1979; A rapid alkaline extraction procedure for screening recombinant DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. CHO H. Y., TANIZAWA K., TANAKA H., SODA K. 1987; Thermostable aminoacylase from Bacillus thermoglucosidus:purification and characterization. Agricultural and Biological Chemistry 51:2793–2800
    [Google Scholar]
  4. CHO H. Y., TANIZAWA K., TANAKA H., SODA K. 1988; Thermostable dipeptidase from Bacillus stearothermophilus:its purification, characterization, and comparison with aminoacylase. Journal of Biochemistry 103:622–628
    [Google Scholar]
  5. DAGERT M., EHRLICH S. D. 1979; Prolonged incubation in calcium chloride improves the competence of E. colicells. Gene 6:23–28
    [Google Scholar]
  6. DAVIS R. H. 1986; Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa Saccharomyces cerevi- siae . Microbiological Reviews 50:280–313
    [Google Scholar]
  7. DEGRYSE E. 1974; Evidence that yeast acetylornithinase is a carboxypeptidase. FEBS Letters 43:285–288
    [Google Scholar]
  8. FALMAGNE P., VANDERWINKEL E., WIAME J. M. 1965; Mise en évidence de deux malate synthétases chez Escherichia coli . Biochimica et Biophysica Acta 99:246–258
    [Google Scholar]
  9. HAAS D., HOLLOWAY B. W., SCHAMBOCK A., LEISINGER T. 1977; The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa . Molecular and General Genetics 154:7–22
    [Google Scholar]
  10. JEENES D. J., SOLDATI L., BAUR H., WATSON J. M., MERCENIER A., REIMMANN C., LEISINGER T., HAAS D. 1986; Expression of biosynthetic genes from Pseudomonas aeruginosa Escherichia coliin heterologous hosts. Molecular and General Genetics 203:421–429
    [Google Scholar]
  11. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning. A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. MEILE L., LEISINGER T. 1984; Enzymes of arginine biosynthesis in methanogenic bacteria. Experientia 40:899–900
    [Google Scholar]
  13. MORRIS C. J., THOMPSON J. F. 1975; Acetyl coenzyme-A glutamate acetyltransferase and N2 -acetylomithine acetyltransferase of Chlorella. Plant Physiology 55:960–967
    [Google Scholar]
  14. MOUNTAIN A., MANN N. H., MUNTON R. N., BAUMBERG S. 1984; Cloning of Bacillus subtilisrestriction fragment complementing auxotrophic mutants of eight Escherichia coligenes of arginine biosynthesis. Molecular and General Genetics 197:82–89
    [Google Scholar]
  15. MOUNTAIN A., MCCHESNEY J., SMITH M. C. M., BAUMBERG S. 1986; Gene sequence encoding early enzymes of arginine synthesis within a cluster in Bacillus subtilis,as revealed by cloning in Escherichia coli . Journal of Bacteriology 165:1026–1028
    [Google Scholar]
  16. NORRANDER J., KEMPE T., MESSING J. 1983; Construction of M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106
    [Google Scholar]
  17. PICARD F.J., DILLON J. R. 1989; Cloning and organization of seven arginine biosynthesis genes from Neisseria gonorrhoeae . Journal of Bacteriology 171:1644–1651
    [Google Scholar]
  18. PIÉRARD A., WIAME J.M. 1964; Regulation and mutation affecting a glutamine dependent formation of carbamylphosphate in Escherichia coli . Biochemical and Biophysical Research Communications 15:76–81
    [Google Scholar]
  19. RAMALEY R. F., HIXSON J. 1970; Isolation of a nonpigmented, thermophilic bacterium similar to Thermus aquaticus . Journal of Bacteriology 103:527–528
    [Google Scholar]
  20. ROSS G. W., O’Callaghan C. H. 1975; β-Lactamase assays. Methods in Enzymology 43:69–85
    [Google Scholar]
  21. SAKANYAN V. A., HOVSEPYAN A. S., METT I. L., KOCHIKYAN A. V., PETROSYAN P. K. 1990; Molecular cloning and structural- functional analysis of the arginine biosynthesis genes of the thermophilic bacterium Bacillus stearothermophilus . Genetika (USSR) 26:1915–1925
    [Google Scholar]
  22. STALON V., RAMOS F., PIÉRARD A., WIAME J. M. 1967; The occurrence of a catabolic and an anabolic ornithine carbamoyltransferase in Pseudomonas . Biochimica et Biophysica Acta 139:91–97
    [Google Scholar]
  23. UDAKA S. 1966; Pathway-specific pattern of control of arginine biosynthesis in bacteria. Journal of Bacteriology 91:617–621
    [Google Scholar]
  24. VAN DE CASTEELE M., DEMAREZ M., LEGRAIN C., GLANSDORFF N., PIÉRARD A. 1990; Pathways of arginine biosynthesis in extreme thermophilic archaeo- and eubacteria. Journal of General Microbiology 136:1177–1183
    [Google Scholar]
  25. VOGEL H. J., BONNER D. M. 1956; Acetylornithinase of Escherichia coli :partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  26. VOGEL H. J., MCLELLAN W.L. 1970; Acetylornithinase (Escherichia coli) . Methods in Enzymology 17A:265–269
    [Google Scholar]
  27. VOGEL R. H., VOGEL H. J. 1963; Acetylated intermediates of arginine synthesis in Bacillus subtilis . Biochimica et Biophysica Acta 69:174–176
    [Google Scholar]
  28. WANNER B. L. 1986; Novel regulatory mutants of the phosphate regulon in Escherichia coliK-12. Journal of Molecular Biology 191:39–58
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-138-1-125
Loading
/content/journal/micro/10.1099/00221287-138-1-125
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error