Resistance of to butyrate and isobutyrate: production and properties of a new anti-isobutyrate (AIB) factor Free

Abstract

Summary: Butyrate and isobutyrate (after isomerization to -butyrate) are specific precursors for the biosynthesis of monensin A in . High concentrations of both butyrate and isobutyrate (greater than 20 and 10 m, respectively) were toxic to plated on solid medium. Spontaneous mutants resistant to these substances were isolated. These new strains produced monensins at even higher concentrations of butyrate or isobutyrate, with an increased yield of monensin A. produced an anti-isobutyrate (AIB) factor, which was originally found to be excreted by some isobutyrate-resistant strains growing on solid medium containing isobutyrate. On plates, the AIB factor efficiently counteracted toxic concentrations not only of isobutyrate, but also of acetate, propionate, butyrate, 2-methylbutyrate, valerate and isovalerate against as well as other species. Although the AIB factor enabled normal growth, sporulation and monensin production on plates, it did not have positive effects on submerged cultures of with isobutyrate. The partial purification of the AIB factor was achieved. The role of the AIB factor during spore germination on solid medium containing isobutyrate or its homologues is discussed.

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1991-09-01
2024-03-29
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References

  1. Boto L., Cano A., Pestaña A. 1987; Biochemical and morphological effects of sodium butyrate on Dictyostelium discoideum development. Molecular and Cellular Biochemistry 74:137–147
    [Google Scholar]
  2. Cullum J., Flett F., Piendl W. 1988; Genetic instability in streptomycetes. Microbiological Sciences 5:233–235
    [Google Scholar]
  3. Day L. E., Chamberlin J. W., Gordee E. Z., Chen S., Gorman M., Hamill R. L., Ness T., Weeks R. E., Stroshane R. 1973; Biosynthesis of monensin. Antimicrobial Agents and Chemotherapy 4:410–414
    [Google Scholar]
  4. Gorman M., Chamberlin J. W., Hamil R. L. 1968; Monensin, a new biologically active compound. V. Compounds related to monensin. Antimicrobial Agents and Chemotherapy 1967:363–368
    [Google Scholar]
  5. Gräfe U. 1989; Autoregulatory secondary metabolites from actinomycetes. Regulation of Secondary Metabolism in Actinomycetes75–126 Shapiro S. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  6. Haney M. E., Hoehn M. M. 1968; Monensin, a new biologically active compound. I. Discovery and isolation. Antimicrobial Agents and Chemotherapy 1967:349–352
    [Google Scholar]
  7. Hesseltine C. W., Benedict R. G., Pridham F. G. 1954; Useful criteria for species differentiation in the genus Streptomyces. Annals of the New York Academy of Sciences 60:136–151
    [Google Scholar]
  8. Kalakoutskii L. V., Agre N. S. 1976; Comparative aspects of development and differentiation in actinomycetes. Bacteriological Reviews 40:469–524
    [Google Scholar]
  9. Khokhlov A. S. 1986; Actinomycete autoregulators. In Biological, Biochemical and Biomedical Aspects of Actinomycetes (FEMS Symposium no. 34)791–798 Szabo G., Biro S., Goodfellow M. Budapest: Akademiai Kiado;
    [Google Scholar]
  10. Kruh J. 1982; Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Molecular and Cellular Biochemistry 42:65–82
    [Google Scholar]
  11. Matějů J., Karnetova J., Nohynek M., Vanek Z. 1988; Propionate and the production of monensins in Streptomyces cinnamonensis. Folia Microbiologica 33:440–446
    [Google Scholar]
  12. Pospíšil S., Zima J. 1987; Biosynthesis of monensins and 3-0-demethylmonensins in Streptomyces cinnamonensis in the presence of methylation inhibitors. FEMS Microbiology Letters 44:283–287
    [Google Scholar]
  13. Pospíšil S., Kralovoova E., Stajner K., Tax J., Krumphanzl V., Vanek Z. 1982; Regulation of biosynthesis of monensins on chemically defined medium. Folia Microbiologica 27:275–277
    [Google Scholar]
  14. Pospíšil S., Sedmera P., Havranek M., Krumphanzl V., Vanek Z. 1983; Biosynthesis of monensin A and B. Journal of Antibiotics 36:617–619
    [Google Scholar]
  15. Pospíšil S., Peterkova M., Krumphanzl V., Vanek Z. 1984; Regulatory mutants of Streptomyces cinnamonensis producing monensin A. FEMS Microbiology Letters 24:209–213
    [Google Scholar]
  16. PospisiL S., Cimburkova E., Krumphanzl V., Vanek Z. 1985; Effect of precursors on biosynthesis of monensins A and B. Folia Microbiologica 30:30–33
    [Google Scholar]
  17. Pospíšil S., Sedmera P., Krumphanzl V., Vanek Z. 1986; Biosynthesis of monensins A and B: the role of isoleucine. Folia Microbiologica 31:8–14
    [Google Scholar]
  18. Pospíšil S., Sedmera P., Matějů J., Nohynek M. 1989; Polyether antibiotics: monensins. In Bioactive Metabolites from Microorganisms Bushell M. E., Gräfe U. Progress in Industrial Microbiology 27:177–192
    [Google Scholar]
  19. Sato K., Nihira T., Sakuda S., Yanagimoto M., Yamada Y. 1989; Isolation and structure of a new butyrolactone autoregulator from Streptomyces sp. FRI-5. Journal of Fermentation and Biotechnology 68:170–173
    [Google Scholar]
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