Summary: In there are mutants (named ) which fail to form aerial mycelium and which are also pleiotropically blocked for synthesis of several antibiotics, including the blue compound actinorhodin. Using a high copy-number vector, a DNA fragment was cloned which restored actinorhodin production, but not aerial mycelium formation, to and mutants. Subcloning and Southern blotting revealed that the fragment was from the region (II) that regulates actinorhodin biosynthesis. The ability to elicit actinorhodin production and to complement an II mutant was localized to a 1·3 kb fragment. These results indicate that the structural genes for actinorhodin biosynthetic enzymes do not contain targets for the and gene products, and suggest that the corresponding genes control actinorhodin synthesis principally via a single gene in the II regulatory region.


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