1887

Abstract

In there are mutants (named ) which fail to form aerial mycelium and which are also pleiotropically blocked for synthesis of several antibiotics, including the blue compound actinorhodin. Using a high copy-number vector, a DNA fragment was cloned which restored actinorhodin production, but not aerial mycelium formation, to and mutants. Subcloning and Southern blotting revealed that the fragment was from the region (II) that regulates actinorhodin biosynthesis. The ability to elicit actinorhodin production and to complement an II mutant was localized to a 1·3 kb fragment. These results indicate that the structural genes for actinorhodin biosynthetic enzymes do not contain targets for the and gene products, and suggest that the corresponding genes control actinorhodin synthesis principally via a single gene in the II regulatory region.

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1991-09-01
2021-10-24
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References

  1. Bruton C. J., Guthrie E. P., Chater K. F. 1991; Phage vectors that allow monitoring of transcription of secondary metabolism genes in Streptomyces . Bio/Technology (in the Press)
    [Google Scholar]
  2. Champness W. C. 1988; New loci required for Streptomyces coelicolor morphological and physiological differentiation. Journal of Bacteriology 170:1168–1174
    [Google Scholar]
  3. Chater K. F. 1972; A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor . Journal of General Microbiology 72:9–28
    [Google Scholar]
  4. Feitelson J. S., Hopwood D. A. 1983; Cloning of a Streptomyces gene for an Omethyltransferase involved in antibiotic biosynthesis. Molecular and General Genetics 190:394–398
    [Google Scholar]
  5. Fernández-Moreno M. A. 1990 Sintesisy regulación de la expresión del antibiotico poliquétido Actinorhodina en Streptomyces coelicolor A3(2). PhD thesis Universidad Autonoma de Madrid;
    [Google Scholar]
  6. Fernández-Moreno M. A., Caballero J. L., Hopwood D. A., Malpartida F. 1991; The actinorhodin gene cluster contains regulatory and antibiotic export genes that are direct targets for translational control by the bldA tRNA gene of Streptomyces coelicolor . Cell
    [Google Scholar]
  7. Guthrie E. P., Chater K. F. 1990; The level of a transcript required for production of a Streptomyces coelicolor antibiotic is conditionally dependent on a tRNA gene. Journal of Bacteriology 172:6189–6193
    [Google Scholar]
  8. Hallam S. E., Malpartida F., Hopwood D. A. 1988; Nucleotide sequence, transcription and deduced function of a gene involved in polyketide antibiotic synthesis in Streptomyces coelicolor . Gene 74:305–320
    [Google Scholar]
  9. Hodgson D. A. 1980 Carbohydrate utilization in Streptomyces coelicolor A3(2). PhD thesis University of East Anglia;
    [Google Scholar]
  10. Hopwood D. A., Wildermuth H., Palmer H. M. 1970; Mutants of Streptomyces coelicolor defective in sporulation. Journal of General Microbiology 61:397–408
    [Google Scholar]
  11. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985a Genetic Manipulation of Streptomyces — a Laboratory Manual Norwich: The John Innes Foundation;
    [Google Scholar]
  12. Hopwood D. A., Chater K. F., Malpartida F. 1985b; Gene cloning to analyse the organisation and expression of antibiotic biosynthesis genes in Streptomyces . Regulation of Secondary Metabolite FormationProceedings of the XV International Congress of Genetics II Recombinant DNA technology22–33 Kleinkauf H., van Dohren H., Dormauer H., Nesseman G. Weinheim: VCH;
    [Google Scholar]
  13. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T. 1987; Plasmid and phage vectors for gene cloning and analysis in Streptomyces . Methods in Enzymology 153:116–166
    [Google Scholar]
  14. Katz E., Thompson C. J., Hopwood D. A. 1988; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans . Journal of General Microbiology 129:2703–2714
    [Google Scholar]
  15. Kieser T., Melton R. 1983; Plasmid pIJ699, a multi-copy positive-selection vector for Streptomyces . Gene 65:83–91
    [Google Scholar]
  16. Lawlor E. J., Baylis H. A., Chater K. F. 1987; Pleiotropic morphological and antibiotic deficiencies result from mutations in a gene encoding a tRNA-like product in Streptomyces coelicolor A3(2). Genes Development 1:1305–1310
    [Google Scholar]
  17. Leskiw B. K., Lawlor E. J., Fernandez-Abalos J. M., Chater K. F. 1991; TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative Streptomyces mutants. Proceedings of the National Academy of Sciences of the United States of America 882461–2465
    [Google Scholar]
  18. Malpartida F., Hopwood D. A. 1986; Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2). Molecular and General Genetics 250:66–73
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Merrick M. J. 1976; A morphological and genetic mapping study of bald colony mutants of Streptomyces coelicolor . Journal of General Microbiology 96:299–315
    [Google Scholar]
  21. Messing J. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  22. Puglia A.-M., Alberti M., Bucca G., Granozzi C, Passantino R., Misuraca F. 1986; Heat shock proteins and differentiation in Streptomyces coelicolor A3(2). Abstracts of the 5th International Symposium on the Genetics of Industrial Micro-organisms Split: Yugoslavia;
    [Google Scholar]
  23. Rudd B. A. M., Hopwood D. A. 1979; Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2). Journal of General Microbiology 114:35–43
    [Google Scholar]
  24. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 vectors and host strains: nucleotide sequences of the M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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