Two bacterial strains were isolated with 3-chloroacrylic acid (CAA) as sole source of carbon and energy. Strain CAA1, a Pseudomonas cepacia sp., was capable of growth with only the cis-isomer of CAA. Strain CAA2, a coryneform bacterium, utilized both isomers of CAA as sole source of carbon and energy. Strain CAA1 contained cis-CAA hydratase and strain CAA2 contained two hydratases, one with cis-CAA hydratase activity and one with trans-CAA hydratase activity. The product of the hydratase activities with CAA was malonate semialdehyde. In both strains malonate semialdehyde was subsequently decarboxylated by a cofactor-independent decarboxylase yielding acetaldehyde and CO2.
AlbertyR. A.,
MasseyV.,
FriedenC.,
FuhlbriggeA. R.1954; Studies of the enzyme fumarase. III. The dependence of kinetic constants at 25° upon the concentration and pH of phosphate buffers. Journal of the American Chemical Society 76:2485–2493
BagleyD. M.,
GossettJ. M.1990; Tetrachloroethene transformation to trichloroethene and cis-l,2-dichloroethene by sulfate-reducing enrichment cultures. Applied and Environmental Microbiology 56:2511–2516
BeckerB.,
LechevalierM. P.,
GordonR. E.,
LechevalierH. A.1964; Rapid differentiation between Nocardia and Strepto-myces by paper chromatography of whole-cell hydrolysates. Applied Microbiology 12:421–423
BelserN. O.,
CastroC. E.1971; Biodehalogenation - the metabolism of the nematocides cis- and trans-3-chloroallyl alcohol by a bacterium isolated from soil. Journal of Agricultural and Food Chemistry 19:23–26
FreedmanD. L.,
GossettJ. M.1989; Biological reductive dechlorination of tetrachloroethylene and trichloroethylene to ethylene under methanogenic conditions. Applied and Environmental Microbiology 55:2144–2151
HartmansS.,
De BontJ. A. M.1986; Acetol monooxygenase from Mycobacterium Pyl cleaves acetol into acetate and formaldehyde. FEMS Microbiology Letters 36:155–158
HartmansS.,
JansenM. W.,
De BontJ. A. M.1988; 3-Chloroacrylic acid metabolism in bacteria. Microbial Physiology and the Manufacturing Industry205RatledgeC.,
SzentirmaiA.,
BarabàsG.,
KeveiF.
Budapest: OMIKK;
JonesD.,
KeddieR. M.1986; Genus Brevibacterium
. Bergey's Manual of Systematic Bacteriology21301–1313SneathP. H.A.,
MairN. S.,
SharpeM. E.,
HoltJ. G.
Baltimore: Williams & Wilkins;
KeuningS.,
JanssenD. B.,
WithoutB.1985; Purification and characterization of hydrolytic dehalogenase from Xanthobacter autotrophicus GJ10. Journal of Bacteriology 163:635–639
MarlettaM. A.,
CheungY. F.,
WalshC.1982; Stereochemical studies on the hydration of monofluorofumarate and 2,3-difluoro-fumarate by fumarase. Biochemistry 21:2637–2644
MotosugiK.,
EsakiN.,
SodaK.1982; Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp. Journal of Bacteriology 150:522–527
ScholtzR.,
LeisingerTh.,
SuterF.,
CookA. M.1987; Characterization of 1-chlorohexane halidohydrolase, a dehalogenase of wide substrate range from an Arthrobacter sp. Journal of Bacteriology 169:5016–5021
SmithJ. M.,
HarrisonK.,
ColbyJ.1990; Purification and characterization of D-2-haloacid dehalogenase from Pseudomonas putida strain AJ1/23. Journal of General Microbiology 136:881–886
WackettL. P.,
BrusseauG. A.,
HouseholderS. R.,
HansonR. S.1989; Survey of microbial oxygenases: trichloroethylene degradation by propane-oxidizing bacteria. Applied and Environmental Microbiology 55:2960–2964
YamadaE. W.,
JakobyW. B.1960; Aldehyde oxidation. V. Direct conversion of malonic semialdehyde to acetyl-coenzyme A. Journal of Biological Chemistry 235:589–594
YokotaT.,
FuseH.,
OmoriT.,
MinodaY.1986; Microbial dehalogenation of haloalkanes mediated by oxygenase or halidohydrolase. Agricultural and Biological Chemistry 50:453–460