Seven strains of the formate-utilizing thermophilic methanogen Methanobacterium thermoformicicum were screened for the presence of extrachromosomal DNA. Covalently closed circular plasmid DNA was detected in three strains, Z-245, FTF and THF. No plasmids were found in strains CB12 and SF-4, nor in two new isolates, FF1 and FF3. The plasmid from strain Z-245, designated pFZ1, and that from strain FTF, designated pFZ2, were approximately 10·5 kb in size and contained homologous and similarly sized restriction fragments. A physical map of plasmid pFZ1 was constructed and three KpnI-fragments comprising the entire plasmid were cloned in Escherichia coli using pUC19. Plasmid pFV1 isolated from strain THF was approximately 14 kb in size and contained regions with strong homology to pFZ1 DNA. Using the Methanobacterium formicicum fdhA gene as a hybridization probe the strains of the species M. thermoformicicum could be classified into two major groups.
BalchW. E.,
FoxG. E.,
MagrumL. J.,
WoeseC. R.,
WolfeR. S.1979; Methanogens: re-evaluation of a unique biological group. Microbiological Reviews 43:260–296
BrandisA.,
ThauerR. K.,
StetterK. O.1981; Relatedness of strain ΔH and Marburg of Methanobacterium thermoautotrophicum . Zentralblatt für Bakteriologie. Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung 1) C2311–317
BrownJ. W.,
DanielsC. J.,
ReeveJ. N.1989; Gene structure, organization, and expression in archaebacteria. CRC Critical Reviews in Microbiology 16:287–337
DasSarmaS.,
DamervalT.,
JonesJ. G.,
Tandeau de MarsacN.1987; A plasmid-encoded gas vesicle protein in a halophilic archaebacterium. Molecular Microbiology 1:365–370
MountfortD. O.,
MorschelE.,
BeimbornD. B.,
SchönheitP.1986; Methanogenesis and ATP synthesis in a protoplast system of Methanobacterium thermoautotrophicum . Journal of Bacteriology 168:892–900
ShuberA. P.,
OrrE. C.,
RecnyM. A.,
SchendelP. F.,
MayH. D.,
SchauerN. L.,
FerryJ. G.1986; Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum . Journal of Biological Chemistry 261:12942–12947
TouzelJ. P.,
PetroffD.,
MaestrojuanG. M.,
PrensierG.,
AlbagnacC.1988; Isolation and characterization of a thermophi-lic Methanobacterium able to use formate, the strain FTF. Archives of Microbiology 149:291–296
WoeseC. R.,
KandlerO.,
WheelisM. L.1990; Towards a natural system of organisms: proposal for the domains Archae, Bacteria, and Eucarya. Proceedings of the National Academy of Sciences of the United States of America874576–4579
WoodA. G.,
WhitmanW. B.,
KoniskyJ.1989; Isolation and characterization of an archaebacterial virus like particle from Methanococcus voltae A3. Journal of Bacteriology 171:93–98
ZhaoH.,
WoodA. G.,
WiddelF.,
BryantM. P.1988; An extremely thermophilic Methanococcus from a deep sea hydro-thermal vent and its plasmid. Archives of Microbiology 150:178–183
ZinderS. H.,
KochM.1984; Non-acetoclastic methanogenesis from acetate : acetate oxidation by a thermophilic syntrophic coculture. Archives of Microbiology 138:263–272