1887

Microbiology Society logo

Volume 137, Issue 8

Positive regulation of colonization factor antigen I (CFA/I) production by enterotoxigenic producing the colonization factors CS5 CS6, CS7, CS17, PCFO9, PCFO159:H4 and PCFO166 Free

Abstract

Enterotoxigenic (ETEC) strains of nineteen serogroups which produced colonization factors (coli-surface-associated antigens CS5, CS6, CS7 and CS17, colonization factor antigen CFA/III and putative colonization factors PCFO159:H4, PCFO166 and PCFO9) were tested for hybridization with a DNA probe containing the sequence that regulates expression of CFA/I. Strong colony hybridization, similar to that with the CFA/I-positive control strain H10407, occurred with ETEC strains of serogroups O27, O159 and O169 which produced CS6 antigen, and with all the strains which produced PCFO166 fimbriae. Weak colony hybridization, compared to the control strain, was found with ETEC producing CS5 fimbriae with CS6 antigen, CFA/III fimbriae with CS6 antigen, CS7 fimbriae or PCFO159:H4 fimbriae. CS6-antigen-positive strains of serogroups O79, O89 and O148 and all the CS17-antigen-positive and PCFO9-fimbriae-positive strains were negative in colony hybridization tests with the probe. Plasmid DNA of nine ETEC strains and their colonization-factor-negative derivatives was tested for hybridization with the probe and with ST and LT oligonucleotide probes. The sequences that hybridized with the probe were on the plasmids which coded for enterotoxin production. Fifteen strains were transformed with NTP513, a recombinant plasmid which contains the CFA/I region 1 fimbrial subunit operon but lacks a functional sequence, in order to determine whether DNA in any of these strains could substitute for the sequence in the regulation of production of CFA/I fimbriae. Transformants of five strains which produced the colonization factors CS6, PCFO166, CS5 + CS6, CS7 and PCFO9, and of one strain which was a colonization-factor-negative derivative of the CS5,CS6-producing strain E17018, gave good production of CFA/I fimbriae comparable to the CFA/I-positive control strain H10407. Transformants of two strains, producing PCFO159 fimbriae and CS17 antigen, respectively, gave weak CFA/I production. Transformants of one strain producing CS6 antigen and of six colonization-factor-negative derivatives did not produce CFA/I fimbriae. These results showed that plasmids in seven of eight types of colonization-factor-positive strains contained gene sequences which could substitute functionally for the sequence. Only two of these strains had gene sequences that hybridized strongly with the probe.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1991
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-8-1963
1991-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/8/mic-137-8-1963.html?itemId=/content/journal/micro/10.1099/00221287-137-8-1963&mimeType=html&fmt=ahah

References

  1. Baldini M. M., Kaper J. B., Levine M. M., Candy D. C. A., Moon H. W. 1983; Plasmid-mediated adhesion in enteropathogenic Escherichia coli . Journal of Pediatric Gastroenterology and Nutrition 2:534–538
     [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
     [Google Scholar]
  3. Caron J., Coffield L. M., Scott J. R. 1989; A plasmid-encoded regulatory gene, rns required for expression of CS1 and CS2 adhesins of enterotoxigenic Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 86963–967
     [Google Scholar]
  4. Caron J., Maneval D. R., Kaper J. B., Scott J. R. 1990; Association of rns homologs with colonization factor antigens in clinical Escherichia coli isolates. Infection and Immunity 58:3442–3444
     [Google Scholar]
  5. Cravioto A., Scotland S. M., Rowe B. 1982; Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans. Infection and Immunity 36:189–197
     [Google Scholar]
  6. Evans D. G., Evans D. J. Jr 1978; New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups 06 and 08. Infection and Immunity 21:638–647
     [Google Scholar]
  7. Evans D. G., Silver R. P., Evans D. J., Chase D. G., Gorbach S. L. 1975; Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infection and Immunity 12:656–667
     [Google Scholar]
  8. Gaastra W., Jordi B. J. A. M., Mul E. M. A., Hamers A. M., McConnell M. M., Willshaw G. A., Smith H. R., Van Der Zeijst B. A. M. 1990; A silent regulatory gene cfaD on region 1 of the CFA/I plasmid NTP113 of enterotoxigenic Escherichia coli . Microbial Pathogenesis 9:285–291
     [Google Scholar]
  9. Hale T. L., Sansonetti P. J., Schad P. A., Austin S., Formal S. B. 1983; Characterization of virulence plasmids and plasmid- associated outer membrane proteins in Shigella flexneri. Shigella sonnei and Escherichia coli . Infection and Immunity 40:340–350
     [Google Scholar]
  10. Hamers A. M., Pel H. J., Willshaw G. A., Kusters J. G., Van Der Zeijst B. A. M., Gaastra W. 1989; The nucleotide sequence of the first two genes of the CFA/I fimbrial operon of human enterotoxigenic Escherichia coli . Microbial Pathogenesis 6:297–309
     [Google Scholar]
  11. Heuzenroeder M. W., Elliot T. R., Thomas C. J., Halter R., Manning P. A. 1990; A new fimbrial type (PCF09) on enterotoxigenic Escherichia coli O9:H-LT+ isolated from a case of infant diarrhea in Central Australia. FEMS Microbiology Letters 66:55–60
     [Google Scholar]
  12. Hibberd M. L., Mconnell M. M., Field A. M., Rowe B. 1990; The fimbriae of human enterotoxigenic Escherichia coli strain 334 are related to CS5 fimbriae. Journal of General Microbiology 136:2449–2456
     [Google Scholar]
  13. Honda T., Arita M., Miwatani T. 1984; Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli a possible new colonization factor. Infection and Immunity 43:959–965
     [Google Scholar]
  14. Knutton S., McConnell M. M., Rowe B., McNeish A. S. 1989; Adhesion and ultrastructural properties of human enterotoxigenic Escherichia coli producing CFA/III and CFA/IV. Infection and Immunity 57:3364–3371
     [Google Scholar]
  15. Kushner S. R. 1978; An improved method for transformation of Escherichia coli with ColEl derived plasmids. Genetic Engineering,17–23 Boyer H. W., Nicosia S. Amsterdam: Elsevier/North Holland Biomedical Press;
     [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
     [Google Scholar]
  17. McConnell M. M., Rowe B. 1989; Prevalence of the putative colonization factors CFA/III and PCF0159:H4 in enterotoxigenic Escherichia coli . Journal of Infectious Diseases 159:582–586
     [Google Scholar]
  18. McConnell M. M., Thomas L. V., Willshaw G. A., Smith H. R., Rowe B. 1988; Genetic control and properties of coli surface antigens of colonization factor antigen IV (PCF8775) of enterotoxigenic Escherichia coli . Infection and Immunity 56:1974–1980
     [Google Scholar]
  19. McConnell M. M., Chart H., Field A. M., Hibberd M., Rowe B. 1989a; Characterization of a putative colonization factor (PCF0166) of enterotoxigenic Escherichia coli of serogroup 0166. Journal of General Microbiology 135:1135–1144
     [Google Scholar]
  20. McConnell M. M., Chart H., Rowe B. 1989b; Antigenic homology within human enterotoxigenic Escherichia coli fimbrial colonization factor antigens: CFA/I, coli-surface-associated antigens (CS) 1, CS2, CS4 and CS 17. FEMS Microbiology Letters 61:105–108
     [Google Scholar]
  21. Connell M. M., Hibberd M., Field A. M., Chart H., Rowe B. 1990; Characterization of a new putative colonization factor (CS17) from a human enterotoxigenic Escherichia coli of serotype OH4:H21 which produces only heat-labile enterotoxin. Journal of Infectious Diseases 161:343–347
     [Google Scholar]
  22. McConnell M. M., Hibberd M. L., Penny M. E., Scotland S. M., Cheasty T., Rowe B. 1991; Surveys of human enterotoxigenic Escherichia coli from three different geographical areas for possible colonization factors. Epidemiology and Infection in the Press
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  24. Perez-Casal J., Swartley J. S., Scott J. R. 1990; Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli . Infection and Immunity 58:3594–3600
     [Google Scholar]
  25. Savelkoul P. H. M., Willshaw G. A., McConnell M. M., Smith H R., Hamers A. J., Van Der Zeijst B. A. M., Gaastra W. 1990; Expression of CFA/I fimbriae is positively regulated. Microbial Pathogenesis 8:91–99
     [Google Scholar]
  26. Smith H. R., Willshaw G. A., Rowe B. 1982; Mapping of a plasmid coding for colonization factor antigen 1 and heat-stable enterotoxin production isolated from an enterotoxigenic strain of Escherichia coli . Journal of Bacteriology 149:264–275
     [Google Scholar]
  27. Smith H. R, Scotland S. M., Rowe B. 1985; Genetics of E. coli virulence. The Virulence of Escherichia coli, Society for General Microbiology Special Publication no. 13227–269 Sussman M. London: Academic Press;
     [Google Scholar]
  28. Smyth C. J. 1982; Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype 06:K15:H16 or H isolated from travellers’ and infantile diarrhoea. Journal of General Microbiology 128:2081–2096
     [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  30. Tacket C. O., Maneval D. R., Levine M. M. 1987; Purification, morphology, and genetics of a new fimbrial putative colonization factor of enterotoxigenic Escherichia coli 0159 :H4. Infection and Immunity 55:1063–1069
     [Google Scholar]
  31. Thomas L. V., McConnell M. M., Rowe B., Field A. M. 1985; The possession of three novel coli surface antigens by enterotoxigenic Escherichia coli strains positive for the putative colonization factor PCF8775. Journal of General Microbiology 131:2319–2326
     [Google Scholar]
  32. Thomas L. V., Rowe B., McConnell M. M. 1987; In strains of Escherichia coli 0167 a single plasmid encodes for the coli surface antigens CS5 and CS6 of putative colonization factor PCF8775, heat-stable enterotoxin and colicin la. Infection and Immunity 55:1929–1931
     [Google Scholar]
  33. Thorne G. M., Deneke C. F., Gorbach S. L. 1979; Haemagglutination and adhesiveness of toxigenic Escherichia coli isolated from humans. Infection and Immunity 23:690–699
     [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
     [Google Scholar]
  35. Willshaw G. A., Smith H. R., Anderson E. S. 1979; Application of agarose gel electrophoresis to the characterization of plasmid DNA in drug-resistant enterobacteria. Journal of General Microbiology 114:15–25
     [Google Scholar]
  36. Willshaw G. A., Smith H. R., Rowe B. 1983; Cloning of regions encoding colonization factor antigen 1 and heat-stable enterotoxin in Escherichia coli . FEMS Microbiology Letters 16:101–106
     [Google Scholar]
  37. Willshaw G. A, Smith H. R., McConnell M. M., Rowe B. 1985; Expression of cloned plasmid regions encoding colonization factor antigen 1 (CFA/I) in Escherichia coli. Plasmid 13:8–16
     [Google Scholar]
  38. Willshaw G. A., McConnell M. M., Smith H. R., Rowe B. 1990a Structural and regulatory genes for coli surface associated antigen 4 (CS4) are encoded by separate plasmids in enterotoxigenic Escherichia coli strains of serotype 025: H42 FEMS Microbiology Letters 68:225–260
     [Google Scholar]
  39. Willshaw G. A, Smith H R., McConnell M. M., Gaastra W., Thomas A., Hibberd M., Rowe B. 1990b; Plasmid-encoded production of coli surface-associated antigen 1 (CS1) in a strain of Escherichia coli serotype O139:H28. Microbial Pathogenesis 9:1–11
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-8-1963
Loading
Positive regulation of colonization factor antigen I (CFA/I) production by enterotoxigenic Escherichia coli producing the colonization factors CS5 CS6, CS7, CS17, PCFO9, PCFO159:H4 and PCFO166
Microbiology 137, 1963 (1991); https://doi.org/10.1099/00221287-137-8-1963
/content/journal/micro/10.1099/00221287-137-8-1963
/content/journal/micro/10.1099/00221287-137-8-1963
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error