@article{mbs:/content/journal/micro/10.1099/00221287-137-8-1955, author = "Ott, Manfred and Paul, Messner and Jürgen, Heesemann and Reinhard, Marre and Jörg, Hacker", title = "Temperature-dependent expression of flagella in Legionella", journal= "Microbiology", year = "1991", volume = "137", number = "8", pages = "1955-1961", doi = "https://doi.org/10.1099/00221287-137-8-1955", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-8-1955", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Legionella pneumophila, the causative agent of Legionnaires' disease, was analysed by electron microscopy for production of surface structures. Crystalline surface (S-) layers and fimbriae were not detected, but monotrichous flagellation was seen. Polyclonal antibodies specific for the 47 kDa flagellin subunit of L. pneumophila Philadelphia I were used in Western blots to confirm the presence of flagella subunits in various L. pneumophila strains tested, but the antiserum also reacted with flagellin subunits of L. micdadei, L. hackelia [serogroup (SG) 1 and SG2] and L. longbeachae (SG2). Flagellation of Legionellae was shown to be temperature regulated. When the growth temperature of virulent and avirulent variants of strain L. pneumophila Philadelphia I was shifted from 30°C to either 37 or 41°C, a decrease in the percentage of flagellated bacteria within the population was observed.", }