, the causative agent of Legionnaires' disease, was analysed by electron microscopy for production of surface structures. Crystalline surface (S-) layers and fimbriae were not detected, but monotrichous flagellation was seen. Polyclonal antibodies specific for the 47 kDa flagellin subunit of Philadelphia I were used in Western blots to confirm the presence of flagella subunits in various strains tested, but the antiserum also reacted with flagellin subunits of [serogroup (SG) 1 and SG2] and (SG2). Flagellation of was shown to be temperature regulated. When the growth temperature of virulent and avirulent variants of strain Philadelphia I was shifted from 30°C to either 37 or 41°C, a decrease in the percentage of flagellated bacteria within the population was observed.


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