1887

Abstract

Survival of strain 168 containing plasmid pAB224, which carries a gene for tetracycline resistance, was studied in mushroom compost under mesophilic and thermophilic conditions. Stable populations of were maintained as spores in both sterile and fresh mushroom compost incubated at 37°C. At 65°C, the introduced populations declined during incubation but spores were still detectable after 28 d. Survival at the higher temperature was greater in fresh than in sterile compost. There was no apparent loss of plasmid pAB224 or plasmid-determined phenotype from the introduced population at either incubation temperature. The frequency of tetracycline resistance in the indigenous population was very low (10), but some tetracycline-resistant isolates contained plasmid DNA. Four plasmid DNA profiles were found associated with five phenotypes, and some evidence for homology with pAB224 was found. However, pAB224 was found to be a suitable marker for release studies because it was easily recovered, readily distinguished from indigenous plasmids on agarose gels, and was maintained in compost-grown 168 in the absence of any selective pressure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-8-1931
1991-08-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/8/mic-137-8-1931.html?itemId=/content/journal/micro/10.1099/00221287-137-8-1931&mimeType=html&fmt=ahah

References

  1. Acea M. J., Moore C. R., Alexander M. 1988; Survival and growth of bacteria introduced into soil. Soil Biology and Biochemistrys 20:509–515
    [Google Scholar]
  2. Aiba S., Kitai K., Imanaka T. 1983; Cloning and expression of thermostable a-amylase gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis. Applied and Environmental Microbiology 46:1059–1065
    [Google Scholar]
  3. Amner W., McCarthy A. J., Edwards C. 1988; Quantitative assessment of factors affecting the recovery of indigenous and released thermophilic bacteria from compost. Applied and Environmental Microbiology 54:3107–3112
    [Google Scholar]
  4. Bentjen S. A., Fredrickson J. K., Van Voris P., Li S. W. 1989; Intact soil-core microcosms for evaluating the fate and ecological impact of the release of genetically engineered microorganisms. Applied and Environmental Microbiology 55:198–202
    [Google Scholar]
  5. Bingham A. H. A., Bruton C. J., Atkinson T. 1979; Isolation and partial characterization of four plasmids from antibiotic-resistant thermophilic bacilli. Journal of General Microbiology 114:401–408
    [Google Scholar]
  6. Bingham A. H. A., Bruton C. J., Atkinson T. 1980; Characterization of Bacillus stearothermophilus plasmid pAB124 and construction of deletion variants. Journal of General Microbiology 119:109–115
    [Google Scholar]
  7. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  8. Bleakley B. H., Crawford D. L. 1989; The effects of varying moisture and nutrient levels on the transfer of a conjugative plasmid between Streptomyces species in soil. Canadian Journal of Microbiology 35:544–549
    [Google Scholar]
  9. Casida L. E. 1989; Protozoan response to the addition of bacterial predators and other bacteria in soil. Applied and Environmental Microbiology 55:1857–1859
    [Google Scholar]
  10. De Rossi E., Brigidi P., Riccardi G., Matteuzzi D. 1989; Plasmid screening in thermophilic Bacillus: physical characterization and molecular cloning. Current Microbiology 19:13–19
    [Google Scholar]
  11. Dev anas M. A., Stotzky G. 1986; Fate in soil of a recombinant plasmid carrying a Drosophila gene. Current Microbiology 13:279–283
    [Google Scholar]
  12. Droffner M. L., Yamamoto N. 1985; Isolation of thermophilic mutants of Bacillus subtilis and Bacillus pumilus and transformation of the thermophilic trait to mesophilic strains. Journal of General Microbiology 131:2789–2794
    [Google Scholar]
  13. Fermor T. R., Randle P. E., Smith J. F. 1985; Compost as a substrate and its preparation. The Biology and Technology of the Cultivated Mushroom81–109 Flegg P. B., Spencer D. M., Wood D. A. Chichester: John Wiley;
    [Google Scholar]
  14. Genthner F. J., Chatterjee P., Barkay T., Bourquin A. W. 1988; Capacity of aquatic bacteria to act as recipients of plasmid DNA. Applied and Environmental Microbiology 54:115–117
    [Google Scholar]
  15. Graham J. B., Istock C. A. 1978; Genetic exchange in Bacillus subtilis in soil. Molecular and General Genetics 166:287–290
    [Google Scholar]
  16. Hoshino T., Ikeda T., Furukawa K., Tomizuka N. 1985a; Genetic relationship between pUBllO and antibiotic resistant plasmids obtained from thermophilic bacilli. Canadian Journal of Microbiology 31:614–619
    [Google Scholar]
  17. Hoshino T., Ikeda T., Narushima H., Tomizuka N. 1985b; Isolation and characterization of antibiotic-resistance plasmids in thermophilic bacilli. Canadian Journal of Microbiology 31:339–345
    [Google Scholar]
  18. Imanaka T., Aiba S. 1986; Applied genetics of aerobic thermophiles. Thermophiles. General Molecular and Applied Microbiology159–178 Brock T. D. New York: John Wiley;
    [Google Scholar]
  19. Imanaka T., Fujii M., Aiba S. 1981; Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. Journal of Bacteriology 146:1091–1097
    [Google Scholar]
  20. Imanaka T., Fujii M., Aramori I., Aiba S. 1982; Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis. Journal of Bacteriology 149:824–830
    [Google Scholar]
  21. Krasovsky V. N., Stotzky G. 1987; Conjugation and genetic recombination in Escherichia coli in sterile and nonsterile soil. Soil Biology and Biochemistry 19:631–638
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Miyagawa K., Kanzaki N., Kimura H., Sumino Y., Akiyama S., Nakao Y. 1989; Increased inosine production by a Bacillus subtilis xanthine-requiring mutant derived by insertional inactivation of the IMP dehydrogenase gene. Biotechnology 7:821–824
    [Google Scholar]
  24. Morel J. L., Bitton G., Chaudhry G. R., Awong J. 1989; Fate of genetically modified microorganisms in the corn rhizosphere. Current Microbiology 18:355–360
    [Google Scholar]
  25. Nakamura K., Imanaka T. 1989; Expression of the insecticidal protein gene from Bacillus thuringiensis subsp. aizawai in Bacillus subtilis and in the thermophile Bacillus stearothermophilus by using the a-amylase promoter of the thermophile. Applied and Environmen-tal Microbiology 55:3208–3213
    [Google Scholar]
  26. Payne C. C. 1988; Pathogens for the control of insects: where next?. Philosophical Transactions of the Royal Society London B318:225–248
    [Google Scholar]
  27. Polack J., Novick R. P. 1982; Closely related plasmids from Staphylococcus aureus and soil bacilli. Plasmid 7:152–162
    [Google Scholar]
  28. Priest F. G. 1977; Extracellular enzyme synthesis in the genus Bacillus. Bacteriological Reviews 41:711–753
    [Google Scholar]
  29. Rafii F., Crawford D. L. 1988; Transfer of conjugative plasmids and mobilization of a nonconjugative plasmid between Streptomyces strains on agar and in soil. Applied and Environmental Microbiology 54:1334–1340
    [Google Scholar]
  30. Richaume A., Angle J. S., Sadowsky M. J. 1989; Influence of soil variables on in situ plasmid transfer from Escherichia coli to Rhizobium fredii. Applied and Environmental Microbiology 55:1730–1734
    [Google Scholar]
  31. Stotzky G., Babich H. 1986; Survival of, and genetic transfer by, genetically engineered bacteria in natural environments. Advances in Applied Microbiology 31:93–138
    [Google Scholar]
  32. Trevors J. T., Berg G. 1989; Conjugal RP4 transfer between pseudomonads in soil and recovery of RP4 plasmid DNA from soil. Systematic and Applied Microbiology 11:223–227
    [Google Scholar]
  33. Trevors J. T., Barkay T., Bourguin A. W. 1987; Gene transfer among bacteria in soil and aquatic environments: a review. Canadian Journal of Microbiology 33:191–198
    [Google Scholar]
  34. Van Elsas J. D., Govaert J. M., Van Veen J. A. 1987; Transfer of plasmid pFT30 between bacilli in soil as influenced by bacterial population dynamics and soil conditions. Soil Biology and Biochemistry 19:639–647
    [Google Scholar]
  35. Van Elsas J. D., Trevors J. T., Starodub M. E. 1988; Plasmid transfer in soil and rhizosphere. Risk Assessment for Deliberate Release89–99 Klingmuller W. Berlin: Springer-Verlag;
    [Google Scholar]
  36. Wang Z., Crawford D. L., Pometto A. L., Rafii F. 1989; Survival and effects of wild-type, mutant and recombinant Streptomyces in a soil ecosystem. Canadian Journal of Microbiology 35:535–543
    [Google Scholar]
  37. Wellington E. M. H., Saunders V. A., Cresswell N., Wipat A. 1988; Plasmid transfer between streptomycetes in soil. Biology of Actinomycetes 88300–305 Okami Y., Beppu T., Ogawara H. Tokyo: Japan Scientific Societies Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-8-1931
Loading
/content/journal/micro/10.1099/00221287-137-8-1931
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error