In glucose minimal medium a PTS- strain of Escherichia coli [Δ(ptsH Ptsl crr)] could grow slowly (doubling time, d = 10 h). When the population reached 5 × 106 to 2 × 107 cells ml−1, mutants growing rapidly (d = 1.5h) appeared and rapidly outgrew the initial population. These mutants (EF mutants) do not use a constitutive galactose permease for glucose translocation. They synthesize sufficient pyrroloquinoline quinone (PQQ) to yield a specific activity of glucose dehydrogenase (GDH) equivalent to that found in the parent strain grown in glucose minimal medium supplemented with 1 nM-PQQ. Membrane preparations containing an active GDH oxidized glucose to gluconic acid, which was also present in the culture supernatant of EF strains in glucose minimal medium. Glucose utilization is the only phenotypic trait distinguishing EF mutants from the parent strain. Glucose utilization by EF mutants was strictly aerobic as expected from a PQQ-dependent catabolism. The regulation of PQQ production by E. coli is discussed.
AmeyamaM.,
ShinagawaE.,
MatsushitaK.,
AdachiO.1984; Growth stimulating substance for microorganisms produced by Escherichia coli causing the reduction of the lag phase in microbial growth and identity of the substance with pyrroloquinoline quinone. Agricultural and Biological Chemistry 48:3099–3107
AmeyamaM.,
NonobeM.,
ShinagawaE.,
MatsushitaK.,
TakimotoK.,
AdachiO.1986; Purification and characterization of the quinoprotein dglucose dehydrogenase apoenzyme from Escherichia coli
. Agricultural and Biological Chemistry 50:49–57
AronsonB. D.,
LevinthalM.,
SomervilleR. L.1989; Activation of a cryptic pathway for threonine metabolism via specific IS3-mediated alteration of promoter structure in Escherichia coli
. Journal of Bacteriology 171:5503–5511
BivilleF.,
MazodierP.,
GasserF.,
Van KleefM. A. G.,
DuineJ. A.1988; Physiological properties of a pyrroloquinoline quinone mutant of Methylobacterium organophilum
. FEMS Microbiology Letters 52:53–58
BivilleF.,
TurlinE.,
GasserF.1989; Cloning and genetic analysis of six pyrroloquinoline quinone biosynthesis genes in Methylobacterium organophilum DSM760. Journal of General Microbiology 135:2917–2929
BouvetO. M. M.,
GrimontP. A. D.1988; Extracellular oxidation of D-glucose by some members of the Enterobacteriaceae
. Annales de I'lnstitut Pasteur/Microbiologie 139:59–77
BouvetO. M. M.,
LenormandP.,
GrimontP. A. D.1989; Taxonomic diversity of the D-glucose oxidation pathway in the Enterobacteriaceae
. International Journal of Systematic Bacteriology 39:61–67
Cleton-JansenA. M.,
GoosenN.,
WenzelT. J.,
Van de PutteP.1988; Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme. Journal of Bacteriology 170:2121–2125
DuineJ. A.,
JongejanJ.1990; Pyrroloquinoline quinone : a novel cofactor. Vitamins and Hormones45223–262AuerbachG. D.,
McCormickD. B.
London: Academic Press;
DuineJ. A.,
FrankJ.,
Jzn,
VerwielP. E.1980; Structure and activity of the prosthetic group of methanol dehydrogenase. European Journal of Biochemistry 108:187–192
FrankJ.Jr,
Van KrimpenS. H.,
Van VerwielP. E. J.,
JongejanJ. A.,
MulderA. C.,
DuineJ. A.1989; On the mechanism of inhibition of methanol dehydrogenase by cyclopropane-derived inhibitors. European Journal of Biochemistry 184:187–195
GoosenN.,
VermaasD. A. M.,
Van de PutteP.1987; Cloning of the genes involved in synthesis of coenzyme pyrroloquinoline-quinone from Acinetobacter calcoaceticus
. Journal of Bacteriology 169:303–307
GrimontP. A. D.,
JacksonT. A.,
AgeronE.,
NoonanM. J.1988; Serratia entomophila sp. nov., associated with Amber disease in the New-Zealand grass grub Costelytra zealandica
. International Journal of Systematic Bacteriology 38:1–6
HommesR. W. J.,
PostmaP. W.,
NeijsselO. M.,
TempestD. W.,
DokterP.,
DuineJ. A.1984; Evidence of a quinoprotein glucose dehydrogenase apoenzyme in several strains of Escherichia coli
. FEMS Microbiology Letters 24:329–333
HommesR. W. J.,
Van HellB.,
PostmaP. W.,
NeijsselO. M.,
TempestD. W.1985; The functional significance of glucose dehydrogenase in Klebsiella aerogenes
. Archives of Microbiology 143:163–168
HommesR. W. J.,
LoenenW. A. M.,
NeijsselO. M.,
PostmaP. W.1986; Galactose metabolism in gal mutants of Salmonella typhimurium and Escherichia coli
. FEMS Microbiology Letters 36:187–190
LevyS.,
ZengG.-Q.,
DanchinA.1990; Cyclic AMP synthesis in Escherichia coli strains bearing known deletions of the pts phospho-transferase operon. Gene 86:27–33
LintonJ. D.,
WoodwardS.,
GouldneyD. G.1987; The consequence of stimulating glucose dehydrogenase activity by the addition of PQQ on metabolite production by Agrobacterium radiobacter NCIB11833. Applied Microbiology and Biotechnology 25:357–361
MacLennanD. G.,
OusbyJ. C.,
VaseyR. B.,
CottonN. T.1971; The influence of dissolved oxygen in Pseudomonas AMI grown on methanol in continuous culture. Journal of General Microbiology 69:395–404
MatsushitaK.,
ShinagawaE.,
InoueT.,
AdachiO.,
AmeyamaM.1986; Immunological evidence of two types of PQQ-dependent dglucose dehydrogenase in bacterial membranes and the location of the enzyme in Escherichia coli
. FEMS Microbiology Letters 37:141–144
MatsushitaK.,
NonobeM.,
ShinagawaE.,
AdachiO.,
AmeyamaM.1987; Reconstitution of a pyrroloquinoline quinone-dependent dglucose oxidase respiratory chain of Escherichia coli with cytochrome o oxidase. Journal of Bacteriology 169:205–209
MeulenbergJ. J. M.,
SellinkE.,
LoenenW. A. M.,
RiegmanN. H.,
Van KleefM.,
PostmaP. W.1990; Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli
. FEMS Microbiology Letters 71:337–344
NeijsselO. M.,
TempestD. W.,
PostmaP. W.,
DuineJ. A.,
FrankJ.,
Jzn.
1983; Glucose metabolism by K+limited Klebsiella aerogenes: evidence for the involvement of a quinoprotein glucose dehydrogenase. FEMS Microbiology Letters 20:35–39
PostmaP. W.,
BroekhuizenC. P.,
GeerseR. H.1989; The role of PEP : carbohydrate phosphotransferase system in the regulation of bacterial metabolism. FEMS Microbiology Reviews 63:69–80
RoyA.,
HazizaG.,
DanchinA.1983; Regulation of adenylate cyclase synthesis in Escherichia coli: nucleotide sequence of the control region. EMBO Journal 2:791–797
Van SchieB. J.,
HellingwerfK. J.,
Van DijkenJ. P.,
ElferinkM. G. L.,
Van DijlJ. M.,
KuenenJ. G.,
KoningsW. L.1985; Energy transduction by electron transfer via a pyrrolo-quinoline quinone dependent glucose dehydrogenase in Escherichia coli, Pseudomonas aeruginosa and Acinetobacter calcoaceticus (var. Iwoffi). Journal of Bacteriology 163:493–499
Van SchieB. J.,
de MooyO. H.,
LintonJ. D.,
Van DijkenJ. P.,
KuenenJ. G.1987; PQQ-dependent production of gluconic acid by Acinetobacter, Agrobacterium and Rhizobium species. Journal of General Microbiology 133:867–875