1887

Abstract

The gene in activates transcription of other genes in that species. A cosmid containing cloned DNA that corrected the Nif defect of an mutant of was isolated. Following Tn transposon mutagenesis of the cosmid in , mutant derivatives unable to correct the mutants were obtained in two separate regions of DNA. In addition, mutations close to one of the regions conferred a complex phenotype when introduced into the genome by marker exchange. These mutants induced non-fixing nodules on peas, were slow-growing on media with succinate as C source or nitrate as N source and, when present in biovar , they failed to make melanin, a pigment that is normally synthesized by . bv. . The mutated gene, termed , was fused to (which encodes -glucuronidase); it was found that transcription of was enhanced in microaerobic conditions and that it was expressed at high levels in infection threads in pea nodules.

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1991-07-01
2021-07-31
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References

  1. Bagdasarian M., Lurz R., Ruckert B., Franklin F. C. H., Bagdasarian M. M., Frey J., Timmis K. N. 1981; Specific-purpose plasmid cloning vectors. II. Broad host range, high copy RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas . Gene 16:237–247
    [Google Scholar]
  2. Beringer J. E. 1974; R. factor transfer in Rhizobium leguminosarum . Journal of General Microbiology 84:188–198
    [Google Scholar]
  3. Beynon J. L., Beringer J. E., Johnston A. W. B. 1980; Plasmids and host range in Rhizobium leguminosarum and Rhizobium phaseoli . Journal of General Microbiology 120:421–429
    [Google Scholar]
  4. Borthakur D., Lamb J. W., Johnston A. W. B. 1987; Identification of two classes of Rhizobium phaseoli genes required for melanin synthesis, one of which is required for nitrogen fixation and activates the transcription of the other. Molecular and General Genetics 207:156–160
    [Google Scholar]
  5. Downie J. A., Hombrecher G., Ma Q.-S., Knight C. D., Wells B., Johnston A. W. B. 1983; Cloned nodulation genes of Rhizobium leguminosarum determine host range specificity. Molecular and General Genetics 190:359–365
    [Google Scholar]
  6. Figurski D. H., Helinski D. R. 1979; Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans . Proceedings of the National Academy of Science of the United States of America 761648–1652
    [Google Scholar]
  7. Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. 1982; Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296
    [Google Scholar]
  8. Hawkins F. K. L. 1989; Studies on the nifA gene of Rhizobium leguminosarum. PhD thesis University of East Anglia;
    [Google Scholar]
  9. Hawkins F. K. L., Johnston A. W. B. 1988; Transcription of a Rhizobium leguminosarum biovar phaseoli gene needed for melanin synthesis is activated by nifA of Rhizobium and Klebsiellapneumoniae . Molecular Microbiology 2:331–337
    [Google Scholar]
  10. Jeffersen R. A., Burgess S. M., Hirsh D. 1986; β-Glucuronidase from Escherichia coli as a gene-fusion marker. Proceedings of the National Academy of Sciences of the United States of America 838447–8451
    [Google Scholar]
  11. Jeffersen R. A., Kavanagh T. A., Bevan M. W. 1987; GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal 6:3901–3907
    [Google Scholar]
  12. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978; High frequency transfer of nodulation ability between strains and species of Rhizobium. Nature London: 276634–636
    [Google Scholar]
  13. Johnston A. W. B., Hombrecher G., Brewin N. J., Cooper M. C. 1982; Two transmissible plasmids in Rhizobium leguminosarum strain 300. Journal of General Microbiology 128:85–93
    [Google Scholar]
  14. Lamb J. W., Downie J. A., Johnston A. W. B. 1985; Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod genes. Gene 34:235–241
    [Google Scholar]
  15. Lamb J. W., Hombrecher G., Johnston A. W. B. 1982; Plasmid determined nodulation and nitrogen fixation abilities in Rhizobium phaseoli . Molecular and General Genetics 186:449–452
    [Google Scholar]
  16. Long S. R. 1989; Rhizobium-legume nodulation: life together in the underground. Cell 56:203–214
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Merrick M., Filser M., Kennedy C., Dixon R. 1978; Polarity of mutations induced by insertion of transposons Tn5, Tn7 and Tn10 into the nif gene cluster of Klebsiella pneumoniae . Molecular and General Genetics 165:103–111
    [Google Scholar]
  19. Miller J. H. 1972; Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Newton J. W., Wilsen P. W., Burris R. H. 1953; Direct demonstration of ammonia as an intermediate in nitrogen fixation by Azotobacter . Journal of Biological Chemistry 204:445–451
    [Google Scholar]
  21. Novel G., Novel M. 1973; Mutants d’Escherichia coli K12 affectés pour leur croissance sur methyl β-d-glucuronide : localisation du gene de structure de la β-d-glucuronidase (uidA). Molecular and General Genetics 120:319–335
    [Google Scholar]
  22. Ronson C. W., Nixon B. T., Albright L. M., Ausubel F. M. 1987; Rhizobium meliloti ntrA mutants fail to utilize succinate as a carbon source. Journal of Bacteriology 169:2424–2431
    [Google Scholar]
  23. Rossen L., Shearman C. A., Johnston A. W. B., Downie J. A. 1985; The nodD gene of Rhizobium leguminosarum is autoregulatory and in the presence of plant exudate induces the nod A, B and C genes. EMBO Journal 4:3369–3373
    [Google Scholar]
  24. Ruvkun G. B., Ausubel F. M. 1981; A general method for site-directed mutagenesis in prokaryotes. Nature London: 28985–88
    [Google Scholar]
  25. Santero E., Toukdarian A., Humphrey R., Kennedy C. 1988; Identification and characterization of two nitrogen fixation regulatory regions, nifA and nfrX in Azotobacter vinelandii and Azotobacter chroococum . Molecular Microbiology 2:303–314
    [Google Scholar]
  26. Stachel S. E., Gynheung A., Flores C., Nester E. W. 1985; A Tn3 lacZ transposon for the random generation of β-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium . EMBO Journal 4:891–898
    [Google Scholar]
  27. Toukdarian A., Kennedy C. 1986; Regulation of nitrogen metabolism in Azotobacter vinelandii isolation of ntr and glnA genes and construction of ntr mutants. EMBO Journal 6:399–407
    [Google Scholar]
  28. Wood W. B. 1966; Host specificity of DNA produced by E. coli bacterial mutation affecting the restriction and modification of DNA. Journal of Molecular Biology 16:118–133
    [Google Scholar]
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