1887

Abstract

Summary: H was grown for 4 generation times with various sub-growth-inhibitory concentrations of β-bactam antibiotics specific for particular penicillin-binding proteins (PBPs) - PBP2, clavulanic acid; PBP3, methicillin; PBP4, cefoxitin - and also with the non-specific benzylpenicillin. Isolated cell walls were digested with muramidase and the resulting peptidoglycan fragments were fractionated by HPLC into disaccharide-peptide monomers and cross-linked dimers, trimers, tetramers and greater oligomers. The pattern of relative fragment concentrations with increasing amounts of drug was roughly the same regardless of the antibiotic used, monomers and dimers increasing while trimers and tetramers changed little and oligomers decreased rapidly. The patterns resembled closely those predicted by the ‘random addition’ model for multiple cross-link formation and not all those predicted by the ‘monomer addition’ model. The -acetylation of the peptidoglycan remained essentially unaffected under all these conditions. MR-1, a constitutive producer of PBP2′, gave similar results when treated with methicillin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-7-1661
1991-07-01
2019-11-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-7-1661
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error