Summary: H was grown for 4 generation times with various sub-growth-inhibitory concentrations of β-bactam antibiotics specific for particular penicillin-binding proteins (PBPs) - PBP2, clavulanic acid; PBP3, methicillin; PBP4, cefoxitin - and also with the non-specific benzylpenicillin. Isolated cell walls were digested with muramidase and the resulting peptidoglycan fragments were fractionated by HPLC into disaccharide-peptide monomers and cross-linked dimers, trimers, tetramers and greater oligomers. The pattern of relative fragment concentrations with increasing amounts of drug was roughly the same regardless of the antibiotic used, monomers and dimers increasing while trimers and tetramers changed little and oligomers decreased rapidly. The patterns resembled closely those predicted by the ‘random addition’ model for multiple cross-link formation and not all those predicted by the ‘monomer addition’ model. The -acetylation of the peptidoglycan remained essentially unaffected under all these conditions. MR-1, a constitutive producer of PBP2′, gave similar results when treated with methicillin.


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