@article{mbs:/content/journal/micro/10.1099/00221287-137-7-1641, author = "Nagahama, Kazuhiro and Ogawa, Takahira and Fujii, Takao and Tazaki, Masato and Goto, Masao and Fukuda, Hideo", title = "l-Arginine is essential for the formation in vitro of ethylene by an extract of Pseudomonas syringae", journal= "Microbiology", year = "1991", volume = "137", number = "7", pages = "1641-1646", doi = "https://doi.org/10.1099/00221287-137-7-1641", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-7-1641", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: A system was developed for the formation of ethylene in vitro by an extract of Pseudomonas syringae pv. phaseolicola PK2. The ethylene-forming activity of a cell-free extract of this bacterium measured in a system reported previously was almost completely lost when the cell-free extract was dialysed against potassium phosphate buffer for 24 h at 4 °C. When the fraction of cell-free extract with a molecular mass < 10 kDa (SupI) was added back to the enzyme fraction after gel filtration of the cell-free extract, the enzymic activity increased to about four times that of the gel-filtered crude enzyme. The action of SupI could be reproduced by the addition of l-arginine. The complete system for the formation of ethylene under aerobic conditions in vitro required 0·25 mm-2-oxoglutarate, 0·2 mm-FeSO4, 2 mm-DTT, 10 mm-l-histidine and 0·2 mm-l-arginine. The cofactor specificity was examined by replacing l-arginine or l-histidine with various analogues, but none of them were effective. The components of this system, with the exception of l-histidine, were similar to those of a system derived from the ethylene-producing, plant pathogenic fungus Penicillium digitatum which also produced ethylene in vitro in a reaction dependent on 2-oxoglutarate. The intermediates in the ethylene-forming reaction are postulated and the roles of l-arginine and l-histidine in the formation of ethylene by Ps. syringae are discussed.", }