1887

Abstract

Summary: A system was developed for the formation of ethylene by an extract of pv. PK2. The ethylene-forming activity of a cell-free extract of this bacterium measured in a system reported previously was almost completely lost when the cell-free extract was dialysed against potassium phosphate buffer for 24 h at 4 °C. When the fraction of cell-free extract with a molecular mass < 10 kDa (SupI) was added back to the enzyme fraction after gel filtration of the cell-free extract, the enzymic activity increased to about four times that of the gel-filtered crude enzyme. The action of SupI could be reproduced by the addition of -arginine. The complete system for the formation of ethylene under aerobic conditions required 0·25 mm-2-oxoglutarate, 0·2 m-FeSO, 2 m-DTT, 10 m--histidine and 0·2 mm--arginine. The cofactor specificity was examined by replacing -arginine or -histidine with various analogues, but none of them were effective. The components of this system, with the exception of -histidine, were similar to those of a system derived from the ethylene-producing, plant pathogenic fungus which also produced ethylene in a reaction dependent on 2-oxoglutarate. The intermediates in the ethylene-forming reaction are postulated and the roles of -arginine and -histidine in the formation of ethylene by are discussed.

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/content/journal/micro/10.1099/00221287-137-7-1641
1991-07-01
2021-10-17
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