1887

Abstract

Summary: Phosphatidyl transfer between phosphatidylethanolamine, phosphatidylglycerol or phosphatidylserine as donors and primary hydroxyl acceptors including ethanolamine, glycerol, serine and Triton X-100 has been shown to be catalysed by membrane particles derived from strains ATCC 13632 and ATCC 14581. The rate of cardiolipin synthesis from phosphatidylglycerol in the presence of ethanolamine was an order of magnitude greater than that of phosphatidylethanolamine formation. Cardiolipin synthesis from phosphatidylethanolamine in the presence of glycerol was also observed, and was 1·5-fold greater than the formation of phosphatidylglycerol. Similar heat lability, effects of pH and of Triton X-100 for phosphatidyl transfer and cardiolipin synthesis indicate that both reactions were catalysed by cardiolipin synthase.

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/content/journal/micro/10.1099/00221287-137-7-1635
1991-07-01
2024-12-09
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References

  1. Burritt M. F., Henderson T. O. 1975; Properties of a membrane-bound cardiolipin synthetase form Lactobacillus plantarum . Journal of Bacteriology 123:972–977
    [Google Scholar]
  2. Kates M. 1972 Techniques of Lipidology. Isolation, Analysis and Identification of Lipids558–559 Work T. S., Work E. Amsterdam: North-Holland Publishing Company;
    [Google Scholar]
  3. Kaufman A. E., Verma J. N., Goldfine H. 1988; Disappearance of plasmalogen-containing phospholipids in Megasphaera elsdenii . Journal of Bacteriology 170:2770–2774
    [Google Scholar]
  4. Langley K. E., Yaffe M. P., Kennedy E. P. 1979; Biosynthesis of phospholipids in Bacillus megaterium . Journal of Bacteriology 140:996–1007
    [Google Scholar]
  5. Lombardi F. J., Fulco A. J. 1980; Two distinct pools of membrane phosphatidylglycerol in Bacillus megaterium . Journal of Bacteriology 141:618–625
    [Google Scholar]
  6. Lombardi F. J., Chen S. L., Fulco A. J. 1980; A rapidly metabolizing pool of phosphatidylglycerol as a precursor for phosphatidylethanolamine and diglyceride in Bacillus megaterium . Journal of Bacteriology 141:626–634
    [Google Scholar]
  7. Morii H., Goldfine H. 1990; Phosphatidyltransferase of Clostridium butyricum Specificity for diacylphosphoglycerides. Biochimica et Biophysica Acta 1044:394–398
    [Google Scholar]
  8. Pieringer R. A. 1989; Biosynthesis of non-terpenoid lipids. Microbial Lipids 251–114 Ratledge C., Wilkinson S. G. London: Academic Press;
    [Google Scholar]
  9. Pluschke G., Hirota Y., Overath P. 1978; Function of phospholipids in Escherichia coli Characterization of a mutant deficient in cardiolipin synthesis. Journal of Biological Chemistry 253:5048–5055
    [Google Scholar]
  10. Raetz C. R. H. 1978; Enzymology, genetics, and regulation of membrane phospholipid synthesis in Escherichia coli . Microbiological Reviews 42:614–659
    [Google Scholar]
  11. Shibuya I., Yamagoe S., Miyazaki C., Matsuzaki H., Ohta A. 1985; Biosynthesis of novel acidic phospholipid analogs in Escherichia coli . Journal of Bacteriology 161:473–477
    [Google Scholar]
  12. Verma J. N., Goldfine H. 1985; Phosphatidylserine decarboxylase from Clostidium butyricum . Journal of Lipid Research 26:610–616
    [Google Scholar]
  13. Walton P. A., Goldfine H. 1987; Transphosphatidylation activity in Clostridium butyricum Evidence for a secondary pathway by which membrane phospholipids may be synthesized and modified. Journal of Biological Chemistry 262:10355–10361
    [Google Scholar]
  14. Yokota K., Kito M. 1982; Transfer of the phosphatidyl moiety of phosphatidylglycerol to phosphatidylethanolamine in Escherichia coli . Journal of Bacteriology 151:952–961
    [Google Scholar]
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