1887

Abstract

Summary: A novel calcium-binding protein has been purified from the dormant spores of T. Purity of this protein was verified by SDS-PAGE and reversed-phase HPLC. Its calcium-binding ability was verified by a competitive calcium-binding assay using Chelex-100 resin and Ca autoradiography. The protein is heat-stable and is retained by hydrophobic matrices (phenyl-Sepharose) in a calcium-dependent manner. SDS-PAGE and amino acid composition indicate the molecular mass of the protein to be 24 kDa.

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/content/journal/micro/10.1099/00221287-137-7-1619
1991-07-01
2021-05-14
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