Immunological relationships between glucosyltransferases synthesizing insoluble glucan from and Free

Abstract

Summary: The values and isoelectric points of glucosyltransferases synthesizing insoluble glucan (GTF-Is) were determined, and the immunological relationships between them studied. The GTF-I enzymes were from (mutans group serotype a), (mutans group serotypes and ) and (mutans group serotype ). By double immunodiffusion tests, the GTF-I enzymes from the three species possessed a common antigenic determinant; in addition, the GTF-I enzymes of serotypes and shared a further determinant. The serotypes and GTF-I enzymes were immunologically identical. The GTF-I enzymes of serotypes and , and of , had an , of 161000 and isoelectric points of 4·8-4·9, while GTF-I had a lower (150000) and a higher isoelectric point (5·2). This suggests that the GTF-I enzyme may lack a sequence of amino acids which include the determinant shared by and GTF-I enzymes. Antibodies specific to the determinant shared by all four serotypes inhibited the homologous and heterologous enzymes by 94-100%.

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1991-07-01
2024-03-29
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References

  1. Beighton D., Russell R. R. B., Hayday H. 1981; The isolation and characterization of Streptococcus mutans serotype h from dental plaque of monkeys (Macaca fascicularis). Journal of General Microbiology 124:271–279
    [Google Scholar]
  2. Carlsson J. 1970; A levansucrase from Streptococcus mutans. Caries Research 4:97–113
    [Google Scholar]
  3. Ciardi J. E. 1983; Purification and properties of glucosyltransferase from Streptococcus mutans : a review. Glucosyltransferases, Glucans, Sucrose and Dental Caries51–64 Doyle R. J., Ciardi J. E. Washington, DC: IRL Press;
    [Google Scholar]
  4. Ciardi J. E., Beaman A. J., Wittenberger C. L. 1977; Purification, resolution, and interaction of the glucosyltransferases of Streptococcus mutans 6715. Infection and Immunity 18:237–246
    [Google Scholar]
  5. Dubois M. L., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric methods for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Fukui K., Fukui Y., Moriyama Y. 1974; Some immunochemical properties of dextransucrase and invertase from Streptococcus mutans. Infection and Immunity 10:985–990
    [Google Scholar]
  7. Fukui K., Moriyama T., Miyake Y., Mizutani K., Tanaka O. 1982; Purification and properties of glucosyltransferase responsible for water-insoluble glucan synthesis from Streptococcus mutans. Infection and Immunity 37:1–9
    [Google Scholar]
  8. Fukui K., Kokeguchi S., Kato K., Miyake Y., Nogami R., Moriyama T. 1983; Immunochemical properties of glucosyltransferases from Streptococcus mutans. Infection and Immunity 39:762–766
    [Google Scholar]
  9. Fukushima K., Motoda R., Takada K., Ikeda T. 1981; Resolution of Streptococcus mutans glucosyltransferases into two components essential to water-insoluble glucan synthesis. FEBS Letters 128:213–216
    [Google Scholar]
  10. Furuta T., Nisizawa T., Chiba J., Hamada S. 1983; Production of monoclonal antibody against a glucosyltransferase of Streptococcus mutans 6715. Infection and Immunity 41:872–875
    [Google Scholar]
  11. Gibbons R. J., Nygaard M. 1968; Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Archives of Oral Biology 13:1249–1262
    [Google Scholar]
  12. Iacono V. J., Taubman M. A., Smith D. J., Levine M. J. 1975; Isolation and immunochemical characterization of group-specific antigen of Streptococcus mutans 6715. Infection and Immunity 11:117–128
    [Google Scholar]
  13. Kenny A. C., Cole J. A. 1983; Identification of a 1,3-α-glucosyltransferase involved in insoluble glucan synthesis by a serotype c strain of Streptococcus mutans. F EMS Microbiology Letters 16:159–162
    [Google Scholar]
  14. Koga T., Sato S., Yakushiji T., Inoue M. 1983; Separation of insoluble and soluble glucan-synthesizing glucosyltransferases of Streptococcus mutans OMZ176 (serotype d). FEMS Microbiology Letters 16:127–130
    [Google Scholar]
  15. Kumada H., Umemoto T, Onisi M., Tsumori H., Shimamura A., Mukasa H. 1987; Purification and characterization of extracellular glucosyltransferase from Streptococcus mutans serotype b (subspecies rattus). Journal of General Microbiology 133:1435–1441
    [Google Scholar]
  16. Linzer R., Slade H. D. 1974; Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infection and Immunity 10:361–368
    [Google Scholar]
  17. Linzer R., Slade H. D. 1976; Characterization of an anti-glucosyltransferase serum specific for insoluble glucan synthesis by Streptococcus mutans. Infection and Immunity 13:494–500
    [Google Scholar]
  18. McCabe M. M. 1985; Purification and characterization of a primerindependent glucosyltransferase from Streptococcus mutans 6715 mutant 27. Infection and Immunity 50:771–777
    [Google Scholar]
  19. McCabe M. M., Alberts M., Stein J. 1987; Monoclonal antibodies to the extracellular glucosyltransferases from Streptococcus sobrinus 6715. Infection and Immunity 55:1900–1905
    [Google Scholar]
  20. Mukasa H. 1986; Properties of Streptococcus mutans glucosyltransferase. Molecular Microbiology and Immunobiology of Streptococcus mutans121–132 Hamada S., Michalek S. M., Kiyono H., Menaker L., McGhee J. R. Amsterdam: Elsevier Science Publishers B. V;
    [Google Scholar]
  21. Mukasa H., Slade H. D. 1973a; Extraction, purification, and chemical and immunological properties of the Streptococcus mutans group “a” polysaccharide cell wall antigen. Infection and Immunity 8:190–198
    [Google Scholar]
  22. Mukasa H., Slade H. D. 1973b; Mechanism of adherence of the Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infection and Immunity 8:555–562
    [Google Scholar]
  23. Mukasa H., Slade H. D. 1974; Mechanism of adherence of Streptococcus mutans to smooth surfaces. III. Purification and properties of the enzyme complex responsible for adherence. Infection and Immunity 10:1135–1145
    [Google Scholar]
  24. Mukasa H., Shimamura A., Tsumori H. 1979; Effect of salts on water-insoluble glucan formation by glucosyltransferase of Streptococcus mutans. Infection and Immunity 23:564–570
    [Google Scholar]
  25. Mukasa H., Shimamura A., Tsumori H. 1982a; Direct activity stains for glycosidase and glucosyltransferase after isoelectric focusing in horizontal polyacrylamide gel layers. Analytical Biochemistry 123:276–284
    [Google Scholar]
  26. Mukasa H., Shimamura A., Tsumori H. 1982b Purification and characterization of basic glucosyltransferase from Streptococcus mutans serotype c. Biochimica et Biophysica Acta 719:81–89
    [Google Scholar]
  27. Mukasa H., Tsumori H., Shimamura A. 1985; Isolation and characterization of an extracellular glucosyltransferase synthesizing insoluble glucan from Streptococcus mutans serotype c. Infection and Immunity 49:790–796
    [Google Scholar]
  28. Mukasa H., Shimamura A., Tsumori H. 1989; Purification and characterization of cell-associated glucosyltransferase synthesizing insoluble glucan from Streptococcus mutans serotype c. Journal of General Microbiology 135:2055–2063
    [Google Scholar]
  29. Ochiai K., Fukushima K., Shiota T., Ikeda T. 1990; Characterization of monoclonal antibodies against glucosyltransferase synthesizing water-insoluble glucan from Streptococcus sobrinus B13. Journal of Dental Research 69:477–482
    [Google Scholar]
  30. Okahashi N., Nishida Y., Koga T., Hamada S. 1984; Immunochemical characteristics of Streptococcus mutans serotype h carbohydrate antigen. Microbiology and Immunology 28:407–413
    [Google Scholar]
  31. Ouchterlony O. 1958; Diffusion of gel methods for immunological analysis. Progress in Allergy 5:1–9
    [Google Scholar]
  32. Russell R. R. B. 1979; Use of Triton X-100 to overcome the inhibition of fructosyltransferase by SDS. Analytical Biochemistry 97:173–175
    [Google Scholar]
  33. Russell R. R. B. 1981; Non-specific binding of Streptococcus mutans glucan-binding proteins and lipoteichoic acid to immunosorbent columns. FEMS Microbiology Letters 11:279–282
    [Google Scholar]
  34. Russell R. R. B., Donald A. C., Douglas C. W. I. 1983; Fructosyltransferase activity of a glucan-binding protein from Streptococcus mutans. Journal of General Microbiology 129:3243–3250
    [Google Scholar]
  35. Scales W. R., Long L. W., Edwards J. R. 1975; Purification and characterization of a glycosyltransferase complex from the culture broth of Streptococcus mutans FAI. Carbohydrate Research 42:325–338
    [Google Scholar]
  36. Shimamura A., Tsumori H., Mukasa H. 1983; Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g). FEBS Letters 157:79–84
    [Google Scholar]
  37. Shimamura A., Tsuboi K., Nagase T, Ito M., Tsumori H., Mukasa H. 1987; Structural determination of D-fructans from Streptococcus mutans, serotype b, c, e, and f strains, by 13C-n.m.r. spectroscopy. Carbohydrate Research 165:150–154
    [Google Scholar]
  38. Smith D. J., Taubman M. A. 1977; Antigenic relatedness of glucosyltransferase enzymes from Streptococcus mutans. Infection and Immunity 5:91–103
    [Google Scholar]
  39. Somogyi M. 1945; A new reagent for the determination of sugars. Journal of Biological Chemistry 160:61–73
    [Google Scholar]
  40. Terleckyj B., Willett N. P., Shockman G. D. 1975; Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infection and Immunity 11:649–655
    [Google Scholar]
  41. Tsumori H., Shimamura A., Mukasa H. 1983a; Purification and properties of extracellular glucosyltransferases from Streptococcus mutans serotype a. Journal of General Microbiology 129:3251–3259
    [Google Scholar]
  42. Tsumori H., Shimamura A., Mukasa H. 1983b; Comparative study of Streptococcus mutans extracellular glycosyltransferases by isoelectric focusing. Journal of General Microbiology 129:3261–3269
    [Google Scholar]
  43. Tsumori H., Shimamura A., Mukasa H. 1985a; Purification and properties of extracellular glucosyltransferase synthesizing 1,3-α-D-glucan from Streptococcus mutans serotype a. Journal of General Microbiology 131:553–559
    [Google Scholar]
  44. Tsumori H., Shimamura A., Mukasa H. 1985a; Purification and properties of extracellular glucosyltransferase synthesizing 1,6-, 1,3-α-D-glucan from Streptococcus mutans serotype a. Journal of General Microbiology 131:3347–3353
    [Google Scholar]
  45. Tsumori H., Kumada H., Umemoto T., Shimamura A., Mukasa H. 1989; Purification and characterization of extracellular glucosyltransferase synthesizing water-insoluble glucan from Streptococcus rattus. Journal of General Microbiology 135:325–333
    [Google Scholar]
  46. Umesaki Y, Kawai T., Mutai M. 1977; Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans. Applied and Environmental Microbiology 34:115–119
    [Google Scholar]
  47. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  48. Wittenberger C. L., Beaman A. J., Lee L. N. 1978; Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius. Journal of Bacteriology 133:231–239
    [Google Scholar]
  49. Yamashita Y., Hanada N., Itoh-Ando M., Takehara T. 1989; Evidence for the presence of two distinct sites of sucrose hydrolysis and glucosyl transfer activities on 1,3-α-D-glucan synthase of Streptococcus mutans. FEBS Letters 243:343–346
    [Google Scholar]
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