@article{mbs:/content/journal/micro/10.1099/00221287-137-7-1591, author = "Seignole, Didier and Mouricout, Michele and Duval-Iflah, Yvonne and Quintard, Bernadette and Julien, Raymond", title = "Adhesion of K99 fimbriated Escherichia coli to pig intestinal epithelium: correlation of adhesive and non-adhesive phenotypes with the sialoglycolipid content", journal= "Microbiology", year = "1991", volume = "137", number = "7", pages = "1591-1601", doi = "https://doi.org/10.1099/00221287-137-7-1591", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-7-1591", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Evidence for the existence of two phenotypes of piglets born to experimental herds was obtained based on the susceptibility of intestinal brush borders to adhesion of K99-positive Eschenchia coli. The enterocytes of the K99-receptive piglets displayed a characteristic sialoglycolipid pattern, with a higher content of the monosialoglyco-lipids II3 NeuGc-LacCer (GM3Gc), IV3NeuGc-nLcOse4Cer (SPGGc) and IV3NeuAc-nLcOse4Cer (SPG) and the oligosialogangliosides IV3NeuAc, II3NeuAc-GgOse4Cer (GD1a), II3(NeuAc)2-GgOse3Cer (GD2), II3 (NeuAc)2-GgOse4Cer (GD1b) and IV3NeuAc, II3(NeuAc)2-GgOse4Cer (GT1b) when compared to the gangliosides of non-receptive piglets. The gangliosides from enterocytes of the non-receptive piglets were mainly the monosialogangliosides II3NeuAc-GgOse3Cer (GM2) and II3NeuAc-LacCer (GM3), only traces of the other sialoglycolipids being detected. Adhesion of 14C-labelled K99-positive E. coli cells to the piglet small intestinal sialoglycolipids, as tested by the thin-layer chromatogram overlay assay, revealed that the receptive enterocyte membrane was richer in glycolipids containing K99 receptor structures than the non-receptive enterocyte. Adhesion of K99-positive E. coli correlated with the degree of sialylation of the brush border glycolipids.", }