Summary: In serum-free medium in the presence of bovine aortal endothelial cells (BAOEC), type b was capable of extensive proliferation compared to that in serum-free medium alone. An unidentified lowmolecular-mass (< 2000 kDa) compound(s) was, in part, responsible for this phenomenon. There were changes in the outer-membrane protein profiles between broth-grown (the original inoculum) and BAOEC-grown organisms, particularly in the 45-70 kDa range. Both broth- and BAOEC-grown bacteria were serum sensitive but could be converted to a serum-resistant phenotype, resembling that found , by incubation in a serum filtrate.


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