Summary: A simple method for filter purification of from cell culture is described. Crude homogenates of chlamydiae-infected cells were passed through a glass prefilter and a 0·6 μm pore diameter polycarbonate filter. The filtrate was then passed through a 0·2 μm pore diameter filter on which the chlamydiae were trapped. This filter was then back-washed to collect the organisms. These procedures removed cell debris and soluble protein, and yielded particles with a narrow size distribution. The mean yield of viable chlamydiae purified by filtration was 64% when the filters were washed at each stage of the process.


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