1887

Abstract

Summary: Bacteriophages were induced from cultures of NCIMB 8303 and ATCC 13541 by UV light. The optimum time of UV exposure was 1 min and the maximum yield of phage was obtained 9-10 h after UV treatment. The two phage preparations were compared by restriction enzyme analysis and Southern blot hybridization. The nucleic acid from both phages was cut by restriction endonucleases specific for double-stranded DNA. The phage DNAs from and showed different restriction enzyme cleavage patterns. No homology was observed between a 25 kb probe from the phage DNA and the phage DNA from . Protein profiles of the phages from both sources were also studied; the phage contained two major bands corresponding to M values of 37000 and 56000 while the phage contained only one major band, of 38000.

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1991-07-01
2021-10-20
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