1887

Abstract

Summary: The genes encoding the 5S ribosomal RNA (rRNA) for strain 3055 were isolated and sequenced. The 5S RNA molecule encoded was 117 nucleotides long. The genome of strain 3055 contained two genes for 5S rRNA that were located close together. The nucleotide sequences of the genes exhibited less similarity to the rRNA gene of strain Moulton and also to those of typical eubacterial genes than did the rRNA genes of other leptospires. However, the overall secondary structure of the 5S rRNA encoded exhibited a strong similarity to that of typical eubacterial 5S rRNA. Southern hybridization of the 5S rRNA gene probe with the genomic DNA of strain 965, which is currently classified as , showed the latter to have close similarity to that of strain 3055. The physical map of strain 965 was quite similar to that of strain 3055 and was greatly different from that of any other strains of . In the organization of 5S rRNA genes, strain 965 is sufficiently different from other members of the genus to be regarded as a member of the genus .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-7-1523
1991-07-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/7/mic-137-7-1523.html?itemId=/content/journal/micro/10.1099/00221287-137-7-1523&mimeType=html&fmt=ahah

References

  1. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J., Zablen L. B., Blakemore R., Gupta R., Bonen L., Lewis B. J., Stahl D. M., Luehrsen K. R., Chen K. N., Woese C. R. 1980; The phylogeny of procaryotes. Science 209:457–463
    [Google Scholar]
  2. Fukunaga M., Mifuchi I. 1988; Mechanism of streptomycin resistance in Leptospira biflexa strain Urawa. Microbiology and Immunology 32:641–644
    [Google Scholar]
  3. Fukunaga M., Mifuchi I. 1989a; The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa . Microbiology and Immunology 33:459–466
    [Google Scholar]
  4. Fukunaga M., Mifuchi I. 1989b; Unique organization of Leptospira interrogans rRNA genes. Journal of Bacteriology 171:5763–5767
    [Google Scholar]
  5. Fukunaga M., Horie I., Mifuchi I. 1990a; Isolation and characterization of the 5S rRNA gene of Leptospira interrogans . Journal of Bacteriology 172:3264–3268
    [Google Scholar]
  6. Fukunaga M., Masuzawa T., Okuzako N., Mifuchi I., Yanagihara Y. 1990b; Linkage of ribosomal RNA genes in Leptospira . Microbiology and Immunology 34:565–573
    [Google Scholar]
  7. Johnson R. C. 1981; Aerobic spirochetes: the genus Leptospira. The Procaryotes582–591 Starr M. P., Stolp H., Truper H. G., Balows A., Schlegel H. G. Berlin: Springer-Verlag;
    [Google Scholar]
  8. Johnson R. C., Faine S. 1984; Leptospiraceae. Bergey’s Manual of Systematic Bacteriology 162–70 Kreig N. R., Holt J. G., Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Marshall R. B., Wilton B. E., Robinson A. J. 1981; Identification of leptospira serovars by restriction-endonuclease analysis. Journal of Medical Microbiology 14:163–166
    [Google Scholar]
  11. Marshall R. B., Winter P. J., Yanagawa R. 1984; Restriction endonuclease DNA analysis of Leptospira interrogans serovar icterohaemorrhagiae and hebdomadis. Journal of Clinical Microbiology 20:808–810
    [Google Scholar]
  12. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Noller H. F. 1984; Structure of ribosomal RNA. Annual Review of Biochemistry 53:119–162
    [Google Scholar]
  14. Paster B. J., Stackebrandt E., Hespell R. B., Hahn C. M., Woese C. R. 1984; The phylogeny of the spirochetes. Systematic and Applied Microbiology 5:337–351
    [Google Scholar]
  15. Perolat P., Grimont F., Regnault B., Grimont P. A. D., Fournie E., Thevenet H., Baranton G. 1990; rRNA gene restriction patterns of Leptospira: a molecular typing system. Research Microbiology 141:159–171
    [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  17. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  18. Thiermann A. B., Handsaker A. L., Moseley S. L., Kingscote B. 1985; New method for classification of leptospiral isolates belonging to serogroup Pomona by restriction endonuclease analysis: serovar kennewicki. Journal of Clinical Microbiology 21:585–587
    [Google Scholar]
  19. Thiermann A. B., Handsaker A. L., Folty J. W., White F. H., Kingscote B. 1986; Reclassification of North American leptospiral isolates belonging to serogroup Mini and Sejroe by restriction endonuclease analysis. American Journal of Veterinary Research 47:61–66
    [Google Scholar]
  20. Woese C. R. 1987; Bacterial evolution. Microbiological Reviews 51:221–271
    [Google Scholar]
  21. Wolters J., Erdmann V. A. 1988; Compilation of 5S rRNA and 5s rRNA gene sequences. Nucleic Acids Research 16:r1–70
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-7-1523
Loading
/content/journal/micro/10.1099/00221287-137-7-1523
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error