1887

Abstract

Glycrol was transported in the fungus var. by a facilitated diffusion transport system with a half-saturation constant, , of 0·5 m and a maximum velocity, , of 0·9 mmol (g dry wt) h at pH 5 and 25 °C. 1,2-Propanediol was a competitive inhibitor of glycerol transport, but the cells did not actively accumulate 1,2-propanediol. The transport system was partially constitutive. In cells grown in the presence of glucose, glycerol was not transported, indicating that the synthesis of the system was under glucose repression. Glycerol kinase and NADP-dependent glycerol dehydrogenase activities were present under all physiological conditions tested. A flavin-dependent glycerol phosphate dehydrogenase was induced only when glycerol was the sole energy source in the medium. This enzyme, together with the transport system, constitute the regulated steps in the glycerol metabolic pathway.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-7-1497
1991-07-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/7/mic-137-7-1497.html?itemId=/content/journal/micro/10.1099/00221287-137-7-1497&mimeType=html&fmt=ahah

References

  1. Adler L., Blomberg A., Nilsson A. 1985; Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii. Journal of Bacteriology 162:300–306
    [Google Scholar]
  2. Andre L., Nilsson A., Adler L. 1988; The role of glycerol in osmotolerance of the yeast Debaryomyces hansenii . Journal of General Microbiology 134:669–677
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. Brandào R. L., Loureiro-Dias M. C. 1990; Regulation of sugar transport systems in Fusarium oxysporum var. lini. Applied and Environmental Microbiology 56:2417–2420
    [Google Scholar]
  5. Burton R. M. 1955; Glycerol dehydrogenase from Aerobacter aerogenes . Methods in Enzymology 1:397–400
    [Google Scholar]
  6. Calmes R., Deal S. J. 1972; Glycerol transport by Nocardia asteroides . Canadian Journal of Microbiology 18:1703–1708
    [Google Scholar]
  7. Courtright J. B. 1975; Intracellular localization and properties of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa . Archives of Biochemistry and Biophysics 167:21–33
    [Google Scholar]
  8. Dawson A. P., Thorne C. J. R. 1975; L-3-Glycerophosphate dehydrogenase from pig brain mitochondria. Methods in Enzymology 41:254–259
    [Google Scholar]
  9. Denor P. F., Courtright J. B. 1978; Isolation and characterization of glycerol-3-phosphate dehydrogenase-defective mutants of Neurospora crassa . Journal of Bacteriology 136:960–968
    [Google Scholar]
  10. Denor P. F., Courtright J. B. 1982; Genetic and enzymatic characterization of the inducible glycerol dissimilatory system of Neurospora crassa . Journal of Bacteriology 151:912–917
    [Google Scholar]
  11. Ferreira K. T. G., Swensson W. M. 1979; The use of 60Co-EDTA as an extracellular marker in frog skin. Biochimica et Biophysica Acta 552:178–182
    [Google Scholar]
  12. Flynn T. G., Cromlish J. A. 1982; Glycerol dehydrogenase from rabbit muscle. Methods in Enzymology 89:237–242
    [Google Scholar]
  13. Funayama S., Gancedo J. M., Gancedo C. 1980; Turnover of yeast fructose-bisphosphatase in different metabolic conditions. European Journal of Biochemistry 109:61–66
    [Google Scholar]
  14. Gancedo C, Gancedo J. M., Sols A. 1968; Glycerol metabolism in yeasts. Pathways of utilization and production. European Journal of Biochemistry 5:165–172
    [Google Scholar]
  15. Gancedo C., Llobell A., Ribas J.-C., Lucre F. 1986; Isolation and characterization of mutants from Schizosaccharomyces pombe defective in glycerol catabolism. European Journal of Biochemistry 159:171–174
    [Google Scholar]
  16. Gartner G., Kopperschlager G. 1984; Purification and properties of glycerol dehydrogenase from Candida valida. Journal of General Microbiology 130:3225–3233
    [Google Scholar]
  17. Jennings D. H. 1984; Polyol metabolism in fungi. Advances in Microbial Physiology 25:149–193
    [Google Scholar]
  18. Jones L. R., Riddick J. A. 1957; Colorimetrie determination of 1,2-propanediol and related compounds. Analytical Chemistry 29:1214–1216
    [Google Scholar]
  19. Klstler W. S., Hirsch C. A., Cozzarelli N. R., Lin E. C. C. 1969; Second pyridine nucleotide-independent lα-glycerophosphate dehydrogenase in Escherichia coli K-12. Journal of Bacteriology 100:1133–1135
    [Google Scholar]
  20. Kistler W. S., Lin E. C. C. 1972; Purification and properties of the flavine-stimulated anaerobic lα-glycerophosphate dehydrogenase of Escherichia coli. Journal of Bacteriology 112:539–547
    [Google Scholar]
  21. Koch J. P., Hayashi S., Lin E. C. C. 1964; The control of dissimilation of glycerol and L-a-glycerophosphate in Escherichia coli . Journal of Biological Chemistry 239:3106–3108
    [Google Scholar]
  22. Kong Y.-C., May J. W., Marshall J. H. 1985; Glycerol oxidation and triose reduction by pyridine nucleotide-linked enzymes in the fission yeast Schizosaccharomyces pombe . Journal of General Microbiology 131:1571–1579
    [Google Scholar]
  23. Loureiro-Dias M. C., Peinado J. M. 1984; Transport of maltose in Saccharomyces cerevisiae Effect of pH and potassium ions. Biochemical Journal 111:293–298
    [Google Scholar]
  24. Lucas C, Costa M., van Uden N. 1990; Osmoregulatory active sodium-glycerol co-transport in the halotolerant yeast Debaryomyces hansenii . Yeast 6:187–191
    [Google Scholar]
  25. May J. W., Sloan J. 1981; Glycerol utilization by Schizosaccharomyces pombe: dehydrogenation as the initial step. Journal of General Microbiology 123:183–185
    [Google Scholar]
  26. Nobre M. F., Costa M. S. 1985; The accumulation of polyols by the yeast Debaryomyces hansenii in response to water stress. Canadian Journal of Microbiology 31:1061–1064
    [Google Scholar]
  27. Sanno Y., Wilson T. H., Lin E. C. C. 1968; Control of permeation to glycerol in cells of Escherichia coli . Biochemical and Biophysical Research Communications 32:344–349
    [Google Scholar]
  28. Sweet G., Gandor C, Voegele R., Wittekindt N., Beuerle J., Truniger V., Lin E. C. C., Boos W. 1990; Glycerol facilitator of Escherichia coli cloning of glpF and identification of the glpF product. Journal of Bacteriology 171:424–430
    [Google Scholar]
  29. Vlsser J., Van Rooijen R., Dljkema C, Swart K., Sealy-Lewis H. M. 1988; Glycerol uptake mutants of the hyphal fungus Aspergillus nidulans . Journal of General Microbiology 134:655–659
    [Google Scholar]
  30. Wltteveen C. F. B., Van De Vondervoort P., Dljkema C., Swart K., Visser J. 1990; Characterization of a glycerol kinase mutant of Aspergillus niger . Journal of General Microbiology 136:1299–1305
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-7-1497
Loading
/content/journal/micro/10.1099/00221287-137-7-1497
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error