Nineteen new Deinococcus isolates from soil, 18 of which were from samples immediately adjacent to a lake in Nottingham, UK, were characterized by conventional criteria, and found to be identical to each other and to conform most closely to the species D. radiopugnans. However, we detected three different restriction enzyme activities in three different isolates. Because of this suggestion of heterogeneity, we examined the isolates for restriction fragment length polymorphisms (RFLPs). RFLP analysis of the 19 original isolates, using three different probes, distinguished 17 divergent groups. This extraordinary diversity cannot be attributed to geographical differences, nor to the method of isolation, which employed UV-radiation selection.
Al-BakriG. H.,
MackayM. W.,
WhittakerP. A.,
MoseleyB. E. B.1985; Cloning of the DNA repair genes mtcA, mtcB, uvsB uvsD, uvsE and the leuB gene from Deinococcus radiodurans
. Gene 33:305–311
AndersonA. W.,
NordenH. C.,
CainR. F.,
ParrishG.,
DugganD.1956; Studies on a radio-resistant micrococcus. I. Isolation, morphology, cultural characteristics, and resistance to gamma radiation. Food Technology 10:575–578
BrooksB. W.,
MurrayR. G. E.1981; Nomenclature for Micrococcus radiodurans and other radiation-resistant cocci : Deinococcaceae fam. nov. and Deinococcus gen. nov including five species. International Journal of Systematic Bacteriology 31:353–360
BrooksB. W.,
MurrayR. G. E.,
JohnsonJ. L.,
StackebrandtE.,
WoeseC. R.,
FoxG. E.1980; Red-pigmented micrococci: a basis for taxonomy. International Journal of Systematic Bacteriology 30:627–646
CaufieldP. W.,
WalkerT. M.1989; Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. Journal of Clinical Microbiology 27:274–278
EvansD. M.,
MoseleyB. E. B.1983; Roles of the uvsC, uvsOuvsE and mtcA genes in the two pyrimidine dimer excision repair pathways of Deinococcus radiodurans
. Journal of Bacteriology 156:576–583
FukushiH.,
HiraiK.1989; Genetic diversity of avian and mammalian Chlamydia psittaci strains and relation to host origin. Journal of Bacteriology 171:2850–2855
HarrisonT. G.,
SaundersN. A.,
HaththotuwaA.,
HallasG.,
BirtlesR. J.,
TaylorA. G.1990; Phenotypic variation amongst genotypically homogeneous Legionella pneumophilia sero-group I isolates : implications for the investigations of outbreaks of Legionaires’ disease. Epidemiology and Infection 104:171–180
HuangY.,
AndersonR.1989; Structure of a novel glucosamine-containing phosphoglycolipid from Deinococcus radiodurans
. Journal of Biological Chemistry 31:18667–18672
ItoH.,
WatanabeH.,
TakehisaM.,
IizukaH.1983; Isolation and identification of radiation-resistant cocci belonging to the genus Deinococcus from sewage sludges and animal feeds. Agricultural and Biological Chemistry 47:1239–1247
Kakoyiannis C,
WinterP. J.,
MarshallR. B.1984; Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis. Applied and Environmental Microbiology 46:545–549
van KetelR. J., ter ScheggeteJ.,
ZanenH. C.1984; Molecular epidemiology of Legionella pneumophilia serogroup I. Journal of Clinical Microbiology 26:362–364
KobatakeM.,
TanabeS.,
HasegawaS.1973; Nouveau Micrococcus radiorésistant à pigment rouge, isolé de féces de Llama glama et son utilisation comme indicateur microbiologique de la radiosterilisation. Comptes Rendus des Séances de la Société de Biologie 167:1506–1510
LennonE.,
MintonK. W.1990; Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans
. Journal of Bacteriology 172:2955–2961
LennonE.,
GutmanP. D.,
YaoH.,
MintonK. W.1991; A highly conserved repeated chromosomal sequence in the radiorésistant bacterium Deinococcus radiodurans SARK. Journal of Bacteriology 173:2137–2140
LiPumaJ. J.,
StullT. L.,
DasenS. E.,
PidcockK. A.,
KayeD.,
KorzeniowskiO. M.1989; DNA polymorphisms among Escherichia coli isolated from bacteriuric women. Journal of Infectious Diseases 159:526–532
MarmurJ.,
DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
MoseleyB. E. B.,
CoplandH. J. R.1978; Four mutants of Micrococcus radiodurans defective in the ability to repair DNA damaged by mitomycin-C, two of which have wild-type resistance to ultraviolet radiation. Molecular and General Genetics 160:331–337
MoseleyB. E. B.,
EvansD. M.1983; Isolation and properties of strains of Micrococcus (Deinococcus) radiodurans unable to excise ultraviolet light-induced pyrimidine dimers from DNA : evidence for two excision pathways. Journal of General Microbiology 129:2437–2445
MurrayR. G. E.1986; The biology and ecology of the Deinococcacea
. In Perspectives in Microbial Ecology (Proceedings of the IV International Symposium on Microbial Ecology)153–158MegusarF.,
GantarM.
Ljubljana: Slovene Society for Microbiology;
MurrayR. G. E.,
BrooksB. W.1986; Deinococcus. In Bergey’s Manual of Systematic Bacteriology21035–1043SneathP. H. A.,
HoltJ. G.
Baltimore: William Wilkins;
NaumovskiL.,
FriedbergE. C.1982; Molecular cloning of eukaryotic genes required for excision repair of UV-irradiated DNA : isolation and partial characterization of the RAD3 gene of Saccharomyces cerevisiae
. Journal of Bacteriology 152:323–331
SandersS. W.,
MaxcyR. B.1979; Isolation of radiation-resistant bacteria without exposure to radiation. Applied and Environmental Microbiology 38:436–439
SaundersN. A.,
HarrisonT. G.,
HaththotuwaA.,
KachwallaN.,
TaylorA. G.1990; A method for typing strains of Legionella pneumophilia serogroup I by analysis of restriction fragment length polymorphisms. Journal of Medical Microbiology 31:45–55
SmithM. D.,
AbrahamsonR.,
MintonK. W.1989; Shuttle plasmids constructed by the insertion of an Escherichia coli cloning vector into two Deinococcus radiodurans plasmids. Plasmid 22:132–142
SmithM. D.,
McNeilL. B.,
MintonK. W.1990; Plasmid genetics and gene expression in Deinococcus radiodurans
. In Genetic Transformation and Expression125–131ButlerL. O.,
HarwoodC.,
MoseleyB. E. B.
Andover, Hants, UK: Intercept;
SteeleT. W.,
LanserJ.,
SangsterN.1990; Isolation of Legionella longbeachae serogroup I from potting mixes. Applied and Environmental Microbiology 56:49–53
TempestP. R.,
MoseleyB. E. B.1980; Defective excision repair in a mutant of Micrococcus radiodurans hypermutable by some monofunctional alkylating agents. Molecular and General Genetics 179:191–199
TirgariS.,
MoseleyB. E. B.1980; Transformation in Micrococcus radiodurans: measurement of various parameters and evidence for multiple independently segregating genomes per cell. Journal of General Microbiology 119:287–296