1887

Abstract

Nineteen new isolates from soil, 18 of which were from samples immediately adjacent to a lake in Nottingham, UK, were characterized by conventional criteria, and found to be identical to each other and to conform most closely to the species . However, we detected three different restriction enzyme activities in three different isolates. Because of this suggestion of heterogeneity, we examined the isolates for restriction fragment length polymorphisms (RFLPs). RFLP analysis of the 19 original isolates, using three different probes, distinguished 17 divergent groups. This extraordinary diversity cannot be attributed to geographical differences, nor to the method of isolation, which employed UV-radiation selection.

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1991-07-01
2021-10-26
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References

  1. Al-Bakri G. H., Mackay M. W., Whittaker P. A., Moseley B. E. B. 1985; Cloning of the DNA repair genes mtcA, mtcB, uvsB uvsD, uvsE and the leuB gene from Deinococcus radiodurans . Gene 33:305–311
    [Google Scholar]
  2. Anderson A. W., Norden H. C., Cain R. F., Parrish G., Duggan D. 1956; Studies on a radio-resistant micrococcus. I. Isolation, morphology, cultural characteristics, and resistance to gamma radiation. Food Technology 10:575–578
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 31:911–917
    [Google Scholar]
  4. Brooks B. W., Murray R. G. E. 1981; Nomenclature for Micrococcus radiodurans and other radiation-resistant cocci : Deinococcaceae fam. nov. and Deinococcus gen. nov including five species. International Journal of Systematic Bacteriology 31:353–360
    [Google Scholar]
  5. Brooks B. W., Murray R. G. E., Johnson J. L., Stackebrandt E., Woese C. R., Fox G. E. 1980; Red-pigmented micrococci: a basis for taxonomy. International Journal of Systematic Bacteriology 30:627–646
    [Google Scholar]
  6. Caufield P. W., Walker T. M. 1989; Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. Journal of Clinical Microbiology 27:274–278
    [Google Scholar]
  7. Clark-Curtiss J. E., Walsh G. P. 1989; Conservation of genomic sequences among isolates of Mycobacterium leprae . Journal of Bacteriology 171:4844–4851
    [Google Scholar]
  8. Counsell T. C., Murray R. G. E. 1986; Polar lipid profiles of the genus Deinococcus . International Journal of Systematic Bacteriology 36:202–206
    [Google Scholar]
  9. Davis N. S., Silverman G. J., Masurovsky E. B. 1963; Radiation resistant, pigmented coccus isolated from Haddock tissue. Journal of Bacteriology 86:294–298
    [Google Scholar]
  10. Evans D. M., Moseley B. E. B. 1983; Roles of the uvsC, uvsO uvsE and mtcA genes in the two pyrimidine dimer excision repair pathways of Deinococcus radiodurans . Journal of Bacteriology 156:576–583
    [Google Scholar]
  11. Fukushi H., Hirai K. 1989; Genetic diversity of avian and mammalian Chlamydia psittaci strains and relation to host origin. Journal of Bacteriology 171:2850–2855
    [Google Scholar]
  12. Giovannoni S. J., Britschgi T. B., Moyer C. L., Field K. G. 1990; Genetic diversity in Sargasso sea bacterioplankton. Nature London: 34560–63
    [Google Scholar]
  13. Harrison T. G., Saunders N. A., Haththotuwa A., Hallas G., Birtles R. J., Taylor A. G. 1990; Phenotypic variation amongst genotypically homogeneous Legionella pneumophilia sero-group I isolates : implications for the investigations of outbreaks of Legionaires’ disease. Epidemiology and Infection 104:171–180
    [Google Scholar]
  14. Huang Y., Anderson R. 1989; Structure of a novel glucosamine-containing phosphoglycolipid from Deinococcus radiodurans . Journal of Biological Chemistry 31:18667–18672
    [Google Scholar]
  15. Ito H., Watanabe H., Takehisa M., Iizuka H. 1983; Isolation and identification of radiation-resistant cocci belonging to the genus Deinococcus from sewage sludges and animal feeds. Agricultural and Biological Chemistry 47:1239–1247
    [Google Scholar]
  16. Kaualanin S., Lindstrom K. 1989; Restriction fragment length polymorphism analysis of Rhizobium galegae strains. Journal of Bacteriology 171:5561–5566
    [Google Scholar]
  17. Kakoyiannis C, Winter P. J., Marshall R. B. 1984; Identification of Campylobacter coli isolates from animals and humans by bacterial restriction endonuclease DNA analysis. Applied and Environmental Microbiology 46:545–549
    [Google Scholar]
  18. van Ketel R. J., ter Scheggete J., Zanen H. C. 1984; Molecular epidemiology of Legionella pneumophilia serogroup I. Journal of Clinical Microbiology 26:362–364
    [Google Scholar]
  19. Kobatake M., Tanabe S., Hasegawa S. 1973; Nouveau Micrococcus radiorésistant à pigment rouge, isolé de féces de Llama glama et son utilisation comme indicateur microbiologique de la radiosterilisation. Comptes Rendus des Séances de la Société de Biologie 167:1506–1510
    [Google Scholar]
  20. Lennon E., Minton K. W. 1990; Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans . Journal of Bacteriology 172:2955–2961
    [Google Scholar]
  21. Lennon E., Gutman P. D., Yao H., Minton K. W. 1991; A highly conserved repeated chromosomal sequence in the radiorésistant bacterium Deinococcus radiodurans SARK. Journal of Bacteriology 173:2137–2140
    [Google Scholar]
  22. Lewis N. F. 1971; Studies on a radio-resistant coccus isolated from Bombay duck (Harpondon nehereus). Journal of General Microbiology 66:29–35
    [Google Scholar]
  23. LiPuma J. J., Stull T. L., Dasen S. E., Pidcock K. A., Kaye D., Korzeniowski O. M. 1989; DNA polymorphisms among Escherichia coli isolated from bacteriuric women. Journal of Infectious Diseases 159:526–532
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  25. Moseley B. E. B. 1983; Photobiology and radiobiology of Micrococcus (Deinococcus) radiodurans . Photochemistry and Photobiology Reviews 7:223–274
    [Google Scholar]
  26. Moseley B. E. B., Copland H. J. R. 1978; Four mutants of Micrococcus radiodurans defective in the ability to repair DNA damaged by mitomycin-C, two of which have wild-type resistance to ultraviolet radiation. Molecular and General Genetics 160:331–337
    [Google Scholar]
  27. Moseley B. E. B., Evans D. M. 1983; Isolation and properties of strains of Micrococcus (Deinococcus) radiodurans unable to excise ultraviolet light-induced pyrimidine dimers from DNA : evidence for two excision pathways. Journal of General Microbiology 129:2437–2445
    [Google Scholar]
  28. Murray R. G. E. 1986; The biology and ecology of the Deinococcacea . In Perspectives in Microbial Ecology (Proceedings of the IV International Symposium on Microbial Ecology)153–158 Megusar F., Gantar M. Ljubljana: Slovene Society for Microbiology;
    [Google Scholar]
  29. Murray R. G. E., Brooks B. W. 1986; Deinococcus. In Bergey’s Manual of Systematic Bacteriology 21035–1043 Sneath P. H. A., Holt J. G. Baltimore: William Wilkins;
    [Google Scholar]
  30. Naumovski L., Friedberg E. C. 1982; Molecular cloning of eukaryotic genes required for excision repair of UV-irradiated DNA : isolation and partial characterization of the RAD3 gene of Saccharomyces cerevisiae . Journal of Bacteriology 152:323–331
    [Google Scholar]
  31. Sanders S. W., Maxcy R. B. 1979; Isolation of radiation-resistant bacteria without exposure to radiation. Applied and Environmental Microbiology 38:436–439
    [Google Scholar]
  32. Saunders N. A., Harrison T. G., Haththotuwa A., Kachwalla N., Taylor A. G. 1990; A method for typing strains of Legionella pneumophilia serogroup I by analysis of restriction fragment length polymorphisms. Journal of Medical Microbiology 31:45–55
    [Google Scholar]
  33. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  34. Smith M. D., Abrahamson R., Minton K. W. 1989; Shuttle plasmids constructed by the insertion of an Escherichia coli cloning vector into two Deinococcus radiodurans plasmids. Plasmid 22:132–142
    [Google Scholar]
  35. Smith M. D., McNeil L. B., Minton K. W. 1990; Plasmid genetics and gene expression in Deinococcus radiodurans . In Genetic Transformation and Expression125–131 Butler L. O., Harwood C., Moseley B. E. B. Andover, Hants, UK: Intercept;
    [Google Scholar]
  36. Steele T. W., Lanser J., Sangster N. 1990; Isolation of Legionella longbeachae serogroup I from potting mixes. Applied and Environmental Microbiology 56:49–53
    [Google Scholar]
  37. Tempest P. R., Moseley B. E. B. 1980; Defective excision repair in a mutant of Micrococcus radiodurans hypermutable by some monofunctional alkylating agents. Molecular and General Genetics 179:191–199
    [Google Scholar]
  38. Tirgari S., Moseley B. E. B. 1980; Transformation in Micrococcus radiodurans: measurement of various parameters and evidence for multiple independently segregating genomes per cell. Journal of General Microbiology 119:287–296
    [Google Scholar]
  39. Ward D. M., Weller R., Bateson M. M. 1990; 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature London: 34563–65
    [Google Scholar]
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