RT Journal Article SR Electronic(1) A1 Corkill, John E. A1 Hart, Charles A. A1 McLennan, Alexander G. A1 Aspinall, StephenYR 1991 T1 Characterization of a ß-lactamase produced by Pseudomonas paucimobilis JF Microbiology, VO 137 IS 6 SP 1425 OP 1429 DO https://doi.org/10.1099/00221287-137-6-1425 PB Microbiology Society, SN 1465-2080, AB Summary: A novel β-lactamase enzyme produced by a strain of Pseudomonas paucimobilis is described. The enzyme differs from other recorded β-lactamases from Gram-negative aerobic bacteria. It was constitutive, and had the characteristics of a penicillinase. One single band of β-lactamase activity at pI 4.6 was seen on iso-electric focusing. The enzyme had a molecular mass of 30 kDa. The β-lactamase was strongly inhibited by tazobactam, sulbactam and clavulanic acid but not by the thiol residue inhibitors p-chloromercuribenzoate and p-chloromercuriphenylsulphonic acid, or by metallo-enzyme inhibitors. Plasmid DNA was not demonstrable, suggesting that the enzyme was chromosomally encoded., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-6-1425