1887

Abstract

Summary: The effects of ruminal concentrations of CO and O on glucose-stimulated and endogenous fermentation of the rumen isotrichid ciliate were investigated. Principal metabolic products were lactic, butyric and acetic acids, H and CO. Traces of propionic acid were also detected; formic acid present in the incubation supernatants was found to be a fermentation product of the bacteria closely associated with this rumen ciliate. C NMR spectroscopy revealed alanine as a minor product of glucose fermentation by . Glucose uptake and metabolite formation rates were influenced by the headspace gas composition during the protozoal incubations. The uptake of exogenously supplied -glucose was most rapid in the presence of O concentrations typical of those detected (i.e. 1–3 μ). A typical ruminal gas composition (high CO, low O) led to increased butyrate and acetate formation compared to results obtained using O-free N. At a partial pressure of 66 kPa CO in N, increased cytosolic flux to butyrate was observed. At low O concentrations (1–3 μ dissolved in the protozoal suspension) in the absence of CO, increased acetate and CO formation were observed and utilized lactate in the absence of extracellular glucose. The presence of both O and CO in the incubation headspaces resulted in partial inhibition of H production by . Results suggest that at the O and CO concentrations that prevail , the contribution made by to the formation of ruminal volatile fatty acids is greater than previously reported, as earlier measurements were made under anaerobic conditions.

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1991-06-01
2021-10-27
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References

  1. Abe M. , Iriki T. , Tobe N. , Shibui H. . 1981; Sequestration of holotrich protozoa in the reticulo-rumen of cattle. Applied and Environmental Microbiology 41 758 765
    [Google Scholar]
  2. Ankrah P. , Loerch S. C. , Dehority B. A. . 1990; Sequestration, migration and lysis of protozoa in the rumen. Journal of General Microbiology 136 1869 1875
    [Google Scholar]
  3. Bhatia S. K. , Pradhan K. , Singh R. . 1979; Microbial transaminase activities and their relationship with bovine rumen metabolites. Journal of Dairy Science 62 441 446
    [Google Scholar]
  4. Čerkasov J. , Čerkasovová A. , Kulda J. , Vilhelmova D. . 1978; Respiration of hydrogenosomes of Tritrichomonas foetusI. ADP- dependent oxidation of malate and pyruvate. Journal of Biological Chemistry 253 1207 1214
    [Google Scholar]
  5. Chapman A. , Cammack R. , Linstead D. , Lloyd D. . 1986a; Respiration of Trichomonas vaginalis :components detectable by electron paramagnetic resonance spectroscopy. European Journal of Biochemistry 156 193 198
    [Google Scholar]
  6. Chapman A. , Linstead D. J. , Lloyd D. , Williams J. . 1986b; Pyruvate metabolism by trichomonads. Biochemical Society Transactions 14 1274
    [Google Scholar]
  7. Czerkawski J. W. . 1986 An Introduction to Rumen Studies 221 Oxford: Pergamon Press;
    [Google Scholar]
  8. Darling T. N. , Davis G. D. , London R. E. , Blum J. J. . 1987; Products of Leishmania bra z Hens isglucose catabolism : Release of d-lactate and, under anaerobic conditions, glycerol. Proceedings of the National Academy of Sciences of the United States of America 84 7129 7133
    [Google Scholar]
  9. Degn H. , Lundsgaard J. S. . 1980; Dynamic gas mixing techniques. Journal of Biochemical and Biophysical Methods 3 233 242
    [Google Scholar]
  10. Degn H. , Cox R. P. , Lloyd D. . 1985; Continuous measurement of dissolved gases in biochemical samples with the quadrupole mass spectrometer. Methods of Biochemical Analysis 31 165 193
    [Google Scholar]
  11. Dehority B. A. , Tirabasso P. A. . 1989; Factors affecting the migration and sequestration of rumen protozoa in the family Isotrichidae. Journal of General Microbiology 135 539 548
    [Google Scholar]
  12. Edwards M. R. , Gilroy F. V. , Jimenez B. M. , O’Sullivan W. J. . 1989; Alanine is a major end product of metabolism by Giardia lamblia:a proton magnetic resonance study. Molecular and Biochemical Parasitotology 37 19 26
    [Google Scholar]
  13. Ellis J. E. , Lloyd D. , Williams A. G. . 1989a; Protozoal contribution to ruminal oxygen utilization. Biochemistry and Molecular Biology of “Anaerobic” Protozoa 32 41 Lloyd D. , Coombs G. H. , Paget T. A. . Chur: Harwood Academic;
    [Google Scholar]
  14. Ellis J. E. , Williams A. G. , Lloyd D. . 1989b; Oxygen consumption by ruminal microorganisms: protozoal and bacterial contributions. Applied and Environmental Microbiology 55 2583 2587
    [Google Scholar]
  15. Gutierrez J. . 1955; Experiments in the culture and physiology of holotrichs of the bovine rumen. Biochemical Journal 60 516 522
    [Google Scholar]
  16. Gutierrez J. , Hungate R. E. . 1957; Interrelationships between certain bacteria and the rumen ciliate Dasytricha ruminantium . Science 126 511
    [Google Scholar]
  17. Harmeyer J. . 1971; Amino acid metabolism of isolated rumen protozoa (Isotricha prostomaand Isotricha intestinalis). 2Excretion of amino acids. Zeitschrift für Tierphysiologie . Tiererährung und Futter-mittelkunde 28 75 85
    [Google Scholar]
  18. Heald P. J. , Oxford A. E. . 1953; Fermentation of soluble sugars by anaerobic holotrich ciliate protozoa of the genera Isotrichaand Dasytricha . Biochemical Journal 53 506 512
    [Google Scholar]
  19. Hillman K. , Lloyd D. , Williams A. G. . 1985a; Use of a portable mass spectrometer for the measurement of dissolved gas concentrations in ovine rumen liquor in situ . Current Microbiology 12 335 340
    [Google Scholar]
  20. Hillman K. , Lloyd D. , Williams A. G. . 1985b; The effect of oxygen on hydrogen production by rumen holotrich protozoa, as determined by membrane-inlet mass spectrometry. Microbial Gas Metabolism 271 277 Poole R. K. , Dow C. S. . London: Academic Press;
    [Google Scholar]
  21. Hillman K. , Lloyd D. , Williams A. G. . 1988; Interactions between the methanogen Methanosarcina barkeriand rumen holotrich ciliate protozoa. Letters in Applied Microbiology 7 49 53
    [Google Scholar]
  22. Howard B. H. . 1959; The biochemistry of rumen protozoa. I. Carbohydrate fermentation by Dasytrichaand Isotricha . Biochemical Journal 71 671 675
    [Google Scholar]
  23. Hungate R. E. . 1966 The Rumen and its Microbes New York: Academic Press;
    [Google Scholar]
  24. Imai S. , Ogimoto K. . 1978; Scanning electron and fluorescent microscopic studies on the attachment of spherical bacteria to ciliate protozoa in the ovine rumen. Japanese Journal of Veterinary Science 40 9 19
    [Google Scholar]
  25. Lloyd D. , Coombs G. H. . 1989; Aerotolerantly anaerobic protozoa: unsolved problems. Biochemistry and Molecular Biology of “Anaerobic” Protozoa 267 286 Lloyd D. , Coombs G. H. , Paget T. A. . Chur: Harwood Academic;
    [Google Scholar]
  26. Lloyd D. , Scott R. I. . 1985; Mass spectrometric monitoring of dissolved gases. Microbial Gas Metabolism 239 262 Poole R. K. , Dow C. S. . London: Academic Press;
    [Google Scholar]
  27. Lloyd D. , Williams J. , Yarlett N. , Williams A. G. . 1982; Oxygen affinities of the hydrogenosome-containing protozoa Tritrichomonas foetusand Dasytricha ruminantiumdetermined by bacterial bioluminescence. Journal of General Microbiology 128 1019 1022
    [Google Scholar]
  28. Lloyd D. , James C. J. , Lloyd A. L. , Yarlett N. , Yarlett N. C. . 1987; The effects of oxygen on fermentation in Tritrichomonas foetusKV1 and its variant IMR-100 with defective hydrogenosomes. Journal of General Microbiology 133 1181 1186
    [Google Scholar]
  29. London R. E. . 1981; 13C Labelling studies of metabolic regulation. Progress in Nuclear Magnetic Resonance Spectroscopy 20 337 383
    [Google Scholar]
  30. McArthur J. M. , Miltimore J. E. . 1961; Rumen gas analysis by gas-solid chromatography. Canadian Journal of Animal Science 41 187 196
    [Google Scholar]
  31. Muller M. . 1988; Energy metabolism of protozoa without mitochondria. Annual Review of Microbiology 42 465 488
    [Google Scholar]
  32. Müller M. , Lindmark D. G. . 1978; Respiration of Tritrichomonas foetusII. Effect of CoA on pyruvate oxidation. Journal of Biological Chemistry 253 1215 1218
    [Google Scholar]
  33. Newbold C. J. , Williams A. G. , Chamberlain D. C. . 1987; In vitrometabolism of D,L-lactic acid by rumen microorganisms. Journal of the Science of Food and Agriculture 38 9 18
    [Google Scholar]
  34. Paget T. A. , Lloyd D. . 1990; Trichomonas vaginalisrequires traces of oxygen and high concentrations of carbon dioxide for optimum growth. Molecular and Biochemical Parasitology 41 65 73
    [Google Scholar]
  35. Paget T. A. , Raynor M. H. , Shipp D. W. E. , Lloyd D. . 1990; Giardia lambliaproduces alanine anaerobically but not in the presence of oxygen. Molecular and Biochemical Parasitology 42 6368
    [Google Scholar]
  36. Prins R. A. , Prast E. R. . 1973; Oxidation of NADH in a coupled oxidase-peroxidase reaction and its significance for the fermentation in rumen protozoa of the genus Isotricha . Journal of Protozoology 20 471 477
    [Google Scholar]
  37. Prins R. A. , Van Hoven W. . 1977; Carbohydrate fermentation by the rumen ciliate Isotricha prostoma . Protistologica 13 549 556
    [Google Scholar]
  38. Scott R. I. , Yarlett N. , Hillman K. , Williams T. N. , Williams A. G. , Lloyd D. . 1983; Presence of oxygen in rumen liquor and its effects on methanogenesis. Journal of Applied Bacteriology 55 143 149
    [Google Scholar]
  39. Stern M. D. , Hoover W. H. , Leonard J. B. . 1977; Ultrastructure of rumen holotrichs by electron microscopy. Journal of Dairy Science 60 911 918
    [Google Scholar]
  40. Van Hoven W. , Prins R. A. . 1977; Carbohydrate fermentation by the rumen ciliate Dasytricha ruminantium . Protistologica 13 599 606
    [Google Scholar]
  41. Wilhelm E. R. , Battino R. , Wilcock R. J. . 1977; Low pressure solubility of gases in liquid water. Chemical Reviews 77 219 262
    [Google Scholar]
  42. Williams A. G. . 1979; The selectivity of carbohydrate assimilation by the anaerobic rumen ciliate Dasytricha ruminantium . Journal of Applied Bacteriology 47 511 520
    [Google Scholar]
  43. Williams A. G. . 1986; Rumen holotrich ciliate protozoa. Microbiological Reviews 50 25 49
    [Google Scholar]
  44. Williams A. G. , Harfoot C. G. . 1976; Factors affecting the uptake and metabolism of soluble carbohydrates by the rumen ciliate Dasytricha ruminantiumisolated from ovine rumen contents by filtration. Journal of General Microbiology 96 125 136
    [Google Scholar]
  45. Williams A. G. , Morrison I. M. . 1982; Studies on the production of saccharinic acids by the alkaline treatment of young grass and their effectiveness as substrates for mixed rumen microorganisms in vitro . Journal of the Science of Food and Agriculture 33 21 29
    [Google Scholar]
  46. Yarlett N. , Hann A. C. , Lloyd D. , Williams A. G. . 1981; Hydrogenosomes in the rumen protozoon Dasytricha ruminantiumSchuberg. Biochemical Journal 200 365 372
    [Google Scholar]
  47. Yarlett N. , Lloyd D. , Williams A. G. . 1982; Respiration of the rumen ciliate Dasytricha ruminantiumSchuberg. Biochemical Journal 206 259 266
    [Google Scholar]
  48. Yarlett N. , Scott R. I. , Williams A. G. , Lloyd D. . 1983; A note on the effects of oxygen on hydrogen production by the rumen protozoon Dasytricha ruminantium . Journal of Applied Bacteriology 55 359 361
    [Google Scholar]
  49. Yarlett N. , Lloyd D. , Williams A. G. . 1985; Butyrate formation from glucose by the rumen protozoon Dasytricha ruminantium . Biochemical Journal 228 187 192
    [Google Scholar]
  50. Yarlett N. , Orpin C. G. , Munn E. A. , Yarlett N. C. , Greenwood A. . 1986; Hydrogenosomes in the rumen fungus Neocallimastix patriciarum . Biochemical Journal 236 729 739
    [Google Scholar]
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