1887

Abstract

Summary: Restriction fragment length polymorphisms (RFLPs) of rRNA genes were evaluated as a tool for intra-and inter-species differentiation of isolates. RFLPs from a collection of 20 clinical isolates and five ATCC strains representing five spp. ( and ) were obtained by hybridization of Southern blots of III- or RI-digested genomic DNA with three probes: probe A, a 0.98 kb III fragment with a partial 16S rRNA gene sequence from ATCC 27337; probe B, cloned operon in plasmid pKK3535; and probe C, 16S and 23S rRNA. The hybridization patterns varied, but all yielded RFLPs useful for both intra- and inter-species differentiation. RFLPs of clinical isolates were closely related to each other and differed significantly from those of the ATCC type strains. The profiles of differed from those of the other four species studied, and based on the III- and RI-generated RFLPs, the strains in this species are more heterogeneous than the other four species studied.

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1991-06-01
2021-04-16
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References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 1:1513–1523
    [Google Scholar]
  2. Bougault A. M., Rosenblatt J. E., Fitzgerald R. H. 1980; Peptococcus magnus: a significant human pathogen. Annals of Internal Medicine 93:244–248
    [Google Scholar]
  3. Brook I. 1987; Bacterial synergy in pelvic inflammatory disease. Archives of Gynecology and Obstetrics 241:133–143
    [Google Scholar]
  4. Brook I., Walker R. I. 1984; Pathogenicity of anaerobic grampositive cocci. Infection and Immunity 45:320–324
    [Google Scholar]
  5. Brosius J., Ullrich A., Raker M. A., Gray A., Dull T. J., Gutell R. R., Noller H. F. 1981; Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 6:112–118
    [Google Scholar]
  6. Ezaki T., Yamamoto N., Ninomiyer K., Suzuki S., Yabuuchi E. 1983; Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii, and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. International Journal of Systematic Bacteriology 33:683–698
    [Google Scholar]
  7. Finegold S. M. 1989; General aspects of anaerobic infection. Anaerobic Infections in Humans137–153 Finegold S. M., George W. L. San Diego: Academic Press;
    [Google Scholar]
  8. Garber G. E., Chow A. W. 1989; Female genital tract infections. In Anaerobic Infections in Humans429–453 Finegold S. M., George W. L. San Diego: Academic Press;
    [Google Scholar]
  9. Grimont F., Grimont P. A. D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Annales de l’Institut Pasteur/Microbiologie I37B:165–175
    [Google Scholar]
  10. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4. Blacksburg: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  11. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences of the United States of America 826955–6959
    [Google Scholar]
  12. Mardh P.-A., Westrom L. 1984; Factors that might influence the outcome of studies on the aetiology and epidemiology of acute pelvic inflammatory disease. Scandinavian Journal of Gastroenterology 19:73–86
    [Google Scholar]
  13. Moore L. V. H., Johnson J. L., Moore W. E. C. 1986; Genus Peptostreptococcus Kluyver and van Niel 1936, 401. Bergey’s Manual of Systematic Bacteriology 21083–1092 Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  14. Murdoch D. A., Mitchelmore I. J., Tabaqchali S. 1988; Identification of Gram-positive anaerobic cocci by use of systems for detecting pre-formed enzymes. Journal of Medical Microbiology 25:289–293
    [Google Scholar]
  15. Ng L.-K., Dillon J. R. 1989; Plasmid analysis and antimicrobial susceptibilities of Peptostreptococcus species. FEMS Microbiology Letters 61:47–52
    [Google Scholar]
  16. PÉROLAT P., Grimont F., Regnault B., Grimont P. A. D., Fournie E., Thevenet H., Baranton G. 1990; rRNA gene restriction patterns of Leptospira; a molecular typing system. Research in Microbiology 141:159–171
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Smith G. L. F., Cumming C. G., Ross P. W. 1986; Immunochemical characterization of the cell surface carbohydrate antigens of Peptostreptococcus anaerobius. Journal of General Microbiology 132:525–530
    [Google Scholar]
  19. Southern E. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  20. Sweet R. L. 1986; Pelvic inflammatory disease. Sexually Transmitted Diseases 13:S192–198
    [Google Scholar]
  21. Yogev D., Halachmi D., Kenny G. E., Razin S. 1988; Distinction of species and strains of mycoplasmas (Mollicutes) by genomic DNA fingerprints with an rRNA gene probe. Journal of Clinical Microbiology 26:1198–1201
    [Google Scholar]
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