1887

Abstract

Summary: Iron-uptake mutants of strain 340 were obtained following treatment with streptonigrin, and one such mutant (Fud14) was characterized. strain Fud14 was unable to grow with human transferrin or haemoglobin as the sole source of iron, but grew normally with heat-inactivated normal human serum or haemin. Internalization of Fe from transferrin by strain Fud14 was only 25% of the parent level. Strain Fud14 (≤1 × 10 c.f.u.) did not grow in subcutaneous chambers implanted in mice, whereas the parent strain was infective at an ID of 4.3 × 10 c.f.u. Supplementation of chambers with either normal human serum or haemin resulted in the establishment of strain Fud14 for at least 240 h post-inoculation. Electroporation of Fud14 with wild-type DNA and selection for growth on medium containing human transferrin resulted in a recombinant (Fud15) that was capable of utilizing haemoglobin, and was virulent in mice. These results suggest that a gonococcal strain defective in the ability to utilize iron sources is not capable of survival .

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1991-06-01
2024-03-29
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References

  1. Ala’Aldeen D. A., Davies H. A., Wall R. A., Borriello S. P. 1990; The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor. FEMS Microbiology Letters 69:37–42
    [Google Scholar]
  2. Archibald F. S., , & DeVoe I. W. 1979; Removal of iron from human transferrin by Neisseria meningitidis. FEMS Microbiology Letters 6:159–162
    [Google Scholar]
  3. Arko R. J. 1989; Animal models for pathogenic Neisseria species. Clinical Microbiology Reviews 2:S56–S59
    [Google Scholar]
  4. Arko R. J., Balows A. 1986; Animal models of experimental gonococcal infection. Experimental Models in Antimicrobial Chemotherapy 1355–369 Zak O., Sande M. A. New York: Academic Press;
    [Google Scholar]
  5. Berish S. B., Chen C. Y., Morse S. A. 1990; Expression of the gonococcal Fbp gene in Escherichia coli. Abstracts from the Annual Meeting of the American Society for Microbiology Anaheim, California, USA: Abstract D150 105
    [Google Scholar]
  6. Bullen J. J. 1981; The significance of iron in infection. Reviews of Infectious Disease 3:1127–1138
    [Google Scholar]
  7. Chandler F. W., Kraus S. J., Watts J. C. 1976; Pathological features of experimental gonococcal infection in mice and guinea pigs. Infection and Immunity 13:909–914
    [Google Scholar]
  8. Chen C. Y. 1988 Membrane proteolipids and iron regulated proteins of Neisseria gonorrhoeae. PhD thesis Oregon Health Sciences University; Portland, Oregon, USA:
    [Google Scholar]
  9. Cone R., Hasan S. K., Loun J. W., Morgan A. R. 1976; The mechanism of the degradation of DNA by streptonigrin. Canadian Journal of Biochemistry 54:219–223
    [Google Scholar]
  10. Corbeil L. B., Wunderlich A. C., Corbeil R. R., McCutchan J. A., Ito J. I. Jr, Braude A. I. 1979; Disseminated gonococcal infection in mice. Infection and Immunity 26:984–990
    [Google Scholar]
  11. Dyer D. W., McKenna W., Woods J. P., Sparling P. F. 1987a; Isolation by streptonigrin enrichment and characterization of a transferrin-specific iron uptake mutant of Neisseria meningitidis. Microbial Pathogenesis 3:351–363
    [Google Scholar]
  12. Dyer D. W., West E. P., Sparling P. F. 1987b; Effects of serum carrier proteins on the growth of pathogenic Neisseria with haem- bound iron. Infection and Immunity 55:2171–2175
    [Google Scholar]
  13. Dyer D. W., West E. P., McKenna W., Thompson S. A., Sparling P. F. 1988; A pleiotropic iron-uptake mutant of Neisseria meningitidis lacks a 70-kilodalton iron regulated protein. Infection and Immunity 56:977–983
    [Google Scholar]
  14. Falk J. E. 1964 Porphyrins and Metalloporphyrins 2181–182 Amsterdam: Elsevier;
    [Google Scholar]
  15. Griffiths E., Stevenson P., Ray A. 1990; Antigenic and molecular heterogeneity of the transferrin-binding protein of Neisseria meningitidis. FEMS Microbiology Letters 69:31–36
    [Google Scholar]
  16. Griffiths E., Humphreys J. 1980; Isolation of enterochelin from peritoneal washings of guinea pigs lethally infected with Escherichia coli. Infection and Immunity 28:286–289
    [Google Scholar]
  17. Griffiths E., Stevenson P., Joyce P. 1983; Pathogenic Escherichia coli express new outer membrane proteins when growing in vivo. FEMS Microbiology Letters 16:95–99
    [Google Scholar]
  18. Griffiths E. 1985; Candidate virulence markers. Virulence of Escherichia coli193–226 Sussman M. London: Academic Press;
    [Google Scholar]
  19. Holbein B. E. 1980; Iron-controlled infection with Neisseria meningitidis in mice. Infection and Immunity 29:886–891
    [Google Scholar]
  20. Holbein B. E. 1981; Enhancement of Neisseria meningitidis infection in mice by addition of iron bound to transferrin. Infection and Immunity 34:120–125
    [Google Scholar]
  21. Keevil C. W., Davies D. B., Spillane B. J., Mahenthiralingam E. 1989; Influence of iron-limited and replete continuous culture on the physiology and virulence of Neisseria gonorrhoeae. Journal of General Microbiology 135:851–863
    [Google Scholar]
  22. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  23. Lee B. C., Schryvers A. B. 1988; Specificity of the lactoferrin and transferrin receptors in Neisseria gonorrhoeae. Molecular Microbiology 2:827–829
    [Google Scholar]
  24. McKenna W. R., Mickelsen P. A., Sparling P. F., Dyer D. W. 1988; Iron uptake from lactoferrin and transferrin by Neisseria gonorrhoeae. Infection and Immunity 56:785–791
    [Google Scholar]
  25. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  26. Mickelsen P. A., Sparling P. F. 1981; Ability of Neisseria gonorrhoeae, Neisseria meningitidis and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infection and Immunity 33:555–564
    [Google Scholar]
  27. Mickelsen P. A., Blackman E., Sparling P. F. 1982; Ability of Neisseria gonorrhoeae, Neisseria meningitidis and Neisseria species to obtain iron from lactoferrin. Infection and Immunity 35:915–920
    [Google Scholar]
  28. Mietzner T. A., Luginbuhl G. H., Sandstrom E., Morse S. A. 1984; Identification of an iron-regulated 37,000-dalton protein in the cell envelope of Neisseria gonorrhoeae. Infection and Immunity 45:410–416
    [Google Scholar]
  29. Mietzner T. A., Barnes R. C., Jeanlouis Y. A., Shafer W. M., Morse S. A. 1986; Distribution of an antigenically related iron- regulated protein among the Neisseria spp. Infection and Immunity 51:60–68
    [Google Scholar]
  30. Mietzner T. A., Bolan G., Schoolnik G. K., Morse S. A. 1987; Purification and characterization of the major iron-regulated protein expressed by pathogenic Neisseria. Journal of Experimental Medicine 165:1041–1057
    [Google Scholar]
  31. Morse S. A., Bartenstein L. 1980; Purine metabolism in Neisseria gonorrhoeae the requirement for hypoxanthine. Canadian Journal of Microbiology 26:13–20
    [Google Scholar]
  32. Neilands J. B. 1981; Microbial iron compounds. Annual Review of Biochemistry 50:715–731
    [Google Scholar]
  33. Payne S. M., Finkelstein R. A. 1975; Pathogenesis and immunology of experimental gonococcal infection: role of iron in virulence. Infection and Immunity 12:1313–1318
    [Google Scholar]
  34. Schryvers A. B. 1988; Characterization of the human transferrin and lactoferrin receptors in Haemophilus influenzae. Molecular Microbiology 2:467–472
    [Google Scholar]
  35. Schryvers A. B., Morris L. J. 1988a; Identification and characterization of the transferrin receptor from Neisseria meningitidis. Molecular Microbiology 2:281–288
    [Google Scholar]
  36. Schryvers A. B., Morris L. J. 1988b); Identification and characterization of the human lactoferrin-binding protein of Neisseria meningitidis. Infection and Immunity 56:1144–1149
    [Google Scholar]
  37. Schryvers A. B., Lee B. C. 1989; Comparative analysis of the transferrin and lactoferrin-binding proteins in the family Neisseria- ceae. Canadian Journal of Microbiology 35:409–415
    [Google Scholar]
  38. Sciortino C. V., Finkelstein R. A. 1983; Vibrio cholerae expresses iron-regulated outer membrane proteins in vivo. Infection and Immunity 42:990–996
    [Google Scholar]
  39. Simonson C., Brener D., DeVoe I. W. 1982; Expression of a high affinity mechanism for acquisition of transferrin iron by Neisseria meningitidis. Infection and Immunity 36:107–113
    [Google Scholar]
  40. Tsai J., Dyer D. W., Sparling P. F. 1988; Loss of transferrin receptor activity in Neisseria meningitidis correlates with inability to use transferrin as an iron source. Infection and Immunity 56:3132–3138
    [Google Scholar]
  41. West S. E. H., Sparling P. F. 1987; Aerobactin utilization by Neisseria gonorrhoeae and cloning of a genomic DNA fragment that complements Escherichia coli fhuB mutations. Journal of Bacteriology 169:3414–3421
    [Google Scholar]
  42. West S. E. H., Mickelsen P. A., Dyer D. W., Sparling P. F. 1985; Iron acquisition by the pathogenic Neisseria. The Pathogenic Neisseria415–422 Schoolnik G. A., Brooks G. F., Falkow S., Frasch C. E., Knapp J. S., McCutchan J. A., Morse S. A. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  43. Wong K. H., Arko R. J., Schalla W. O., Steurer F. J. 1979; Immunological and serological diversity of Neisseria gonorrhoeae: Identification of new immunotypes and highly protective strains. Infection and Immunity 23:717–722
    [Google Scholar]
  44. Yancey R. J., Finkelstein R. A. 1981; Assimilation of iron by pathogenic Neisseria spp. Infection and Immunity 32:592–599
    [Google Scholar]
  45. Yeowell H. N., White J. R. 1982; Iron requirement in the bactericidal mechanism of streptonigrin. Antimicrobial Agents and Chemotherapy 22:961–968
    [Google Scholar]
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