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Summary: Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements for activity similar to SalI, and a native M r of about 46000. The DNA-MTase is a protein with an M r of about 67000. No DNA homology was detected between the cloned SalI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.
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