1887

Abstract

Mutants of characterized by osmotic fragility showed a marked fibrillar structure on the inner wall surface when studied by two electron microscopic techniques, i.e. freeze-etching of whole native cells and metal shadowing of isolated cell walls. The walls of the mutant cells were more permeable to macromolecules than were those of the wild-type parental strain. The synthesis and assembly of (1→3)---glucan wall microfibrils studied in protoplasts of mutant cells were not impaired. It is suggested that the osmotic fragility of the mutant cells is related to the deficiency of the wall structure as a consequence of the mutation affecting biogenesis of the amorphous (glucan) component.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-6-1263
1991-06-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/6/mic-137-6-1263.html?itemId=/content/journal/micro/10.1099/00221287-137-6-1263&mimeType=html&fmt=ahah

References

  1. Bacon J. S. D. 1981; Nature and disposition of polysaccharides within the cell envelope. In Yeast Cell Envelopes: Biochemistry, Biophysics and Ultrastructure65–84 Arnold W. N. Boca Raton: CRC Press;
    [Google Scholar]
  2. Bacon J. S. D., Davidson E. D., Jones D., Taylor I. F. 1966; The location of chitin in the yeast cell wall. Biochemical Journal 101:36C–38C
    [Google Scholar]
  3. Blagoeva J., Venkov P. 1990a; Cell surface structures in fragile mutants of Saccharomyces cerevisiae . In Proceedings of the 14th International Specialized Symposium on Yeasts ‘Yeast Taxonomy: Theoretical and Practical Aspects’42–44 Kockova-Kratochvílová A. Smolenice: Institute of Chemistry, Slovak Academy of Sciences; Bratislava:
    [Google Scholar]
  4. Blagoeva J., Venkov P. 1990b; Decreased synthesis of alkali-soluble glucan in a cell-wall mutant of Saccharomyces cerevisiae. Biochemistry International 22:791–795
    [Google Scholar]
  5. Branton D., Bullivant S., Gllula N. B., Karnovsky M. J., Moor H., Mühlethaler K., Northcote D. H., Packer L., Satir B., Satir P., Speth V., Staehelin L. A., Steere R. L., Weinstein R. S. 1975; Freeze-etching nomenclature. Science 190:54–56
    [Google Scholar]
  6. Cabib E., Ulane R., Bowers N. 1974; A molecular model for morphogenesis: the primary septum of yeast. Current Topics in Cellular Regulation 8:1–32
    [Google Scholar]
  7. Cabib E., Roberts R., Bowers B. 1982; Synthesis of the yeast cell wall and its regulation. Annual Review of Biochemistry 51:763–793
    [Google Scholar]
  8. Gabriel M. 1983; Karyokinesis and cell septum formation during incomplete cell wall regeneration in flattened Schizosaccharomyces versatilis protoplasts. In Proceedings of the 6th European Cell Cycle Workshop ‘Progress in Cell Cycle Controls’Prague 1983144–146 Chaloupka J., Kotyk A., Streiblová E. Prague: Institute of Microbiology, Czechoslovak Academy of Sciences;
    [Google Scholar]
  9. Gabriel M. 1984; Karyokinesis and septum formation during the regeneration of incomplete cell walls in protoplasts of Schizosaccharomyces japonicus var. versatilis : a time-lapse microcinematographic study. Journal of General Microbiology 130:625–630
    [Google Scholar]
  10. Gabrlel M., Kopecká M. 1988; Studies on cell division in regenerating protoplasts of the yeast Schizosaccharomyces japonicus . Journal of General Microbiology 134:2029–2037
    [Google Scholar]
  11. Houwink A. L., Kreger D. R. 1953; Observations on the cell wall of yeasts. An electron microscope and X-ray diffraction study. Antonie van Leeuwenhoek 19:1–24
    [Google Scholar]
  12. Kelleti T., Szabolzi G., Lenvai A., Garzo T. 1954; Untersuchungen über die lebens fähigen Eiweisskörper von Saccharomyces cerevisiae Die Regeneration in sterilen Filtrat von zerstorten Hefezellen. Acta Scientifica Academiae Seientiarum Hungaricae 5:213–217
    [Google Scholar]
  13. Kopecká M. 1976; Biogenesis of the fibrillar wall component in yeast protoplasts. PhD thesis (in Czech) J. E. Purkynë; University Brno:
    [Google Scholar]
  14. Kopecká M. 1985; Electron microscopic study of purified polysaccharide components glucan and mannan of the cell walls in the yeast Saccharomyces cerevisiae . Journal of Basic Microbiology 25:161–174
    [Google Scholar]
  15. Kopecká M., Kreger D. R. 1986; Assembly of microfibrils in vivo and in vitro from (1→3)-βdglucan synthesized by protoplasts of Saccharomyces cerevisiae . Archives of Microbiology 143:387–395
    [Google Scholar]
  16. Kopecká M., Čtvrtniček O., Nečas O. 1965; Formation and properties of the fibrillar network formed in yeast protoplasts as the first step of biosynthesis of cell wall. Symposium über Hefe-ProtoplastenJena73–75 Berlin: Akademie Verlag;
    [Google Scholar]
  17. Kopecká M., Phaff H. J., Fleet G. H. 1974; Demonstration of a fibrillar component in the cell wall of the yeast Saccharomyces cerevisiae and its chemical nature. Journal of Cell Biology 62:66–76
    [Google Scholar]
  18. Kozhina T., Stateva L., Venkov P. 1979; Genetic analysis of an osmotic sensitive Saccharomyces cerevisiae mutant. Molecular and General Genetics 170:351–354
    [Google Scholar]
  19. Kreger D. R., Kopecká M. 1973; On the nature of the fibrillar nets formed by protoplasts of Saccharomyces cerevisiae in liquid media. In Yeast, Mold and Plant ProtoplastsProceedings of the Third International Symposium on Yeast ProtoplastsSalamanca117–130 Villanueva J. R., Garcia-Acha I., Gascón S., Uruburu F. London: Academic Press;
    [Google Scholar]
  20. Kreger D. R., Kopecká M. 1976a; On the nature and formation of the fibrillar nets produced by protoplasts of Saccharomyces cerevisiae in liquid media : an electron microscopic, X-ray diffraction and chemical study. Journal of General Microbiology 92:207–221
    [Google Scholar]
  21. Kreger D. R., Kopecká M. 1976b; Assembly of wall polymers during the regeneration of yeast protoplasts. In Microbial and Plant Protoplasts237–252 Peberdy J. F., Rose A. H., Rogers H. J., Cocking E. C. London: Academic Press;
    [Google Scholar]
  22. MÄrkisch U., Reuter G., Stateva L., Venkov P. 1983; Mannan structure analysis of the fragile Saccharomyces cerevisiae mutant VY 1 160. InternationaIJournal of Biochemistry 15:1373–1377
    [Google Scholar]
  23. Mateeva Z., Petrov P., Venkov P., Hadjiolov A. A. 1976; Electron microscopic study of the lysis of an osmotic-sensitive yeast mutant. Journal de Microscopie et de Biologie Cellulaire 26:73–74
    [Google Scholar]
  24. Matile P., Moor H., Robinow C. F. 1969; Yeast cytology. In The Yeasts 1219–297 Rose A. H., Harrison J. S. London & New York: Academic Press;
    [Google Scholar]
  25. Moor H., Mühlethaler K. 1963; Fine structure in frozen-etched yeast cells. Journal of Cell Biology 17:609–628
    [Google Scholar]
  26. Nečas O. 1971; Cell wall synthesis in yeast protoplasts. Bacteriological Reviews 35:149–170
    [Google Scholar]
  27. Phllipova D. H., Venkov P. V. 1990; Cell fusion of Saccharomyces cerevisiae fragile mutants. Yeast 6:205–212
    [Google Scholar]
  28. Reuter G., Märkisch U., Hering V., Venkov P. 1979; Unterschiedliche Zusammensetzung der Zellwand-Polysaccharide bei Saccharomyces cerevisiae S288C und einer osmolabilen Mutante. Zeitschrift für Allgemeine Mikrobiologie 19:411–414
    [Google Scholar]
  29. Stateva L., Venkov P. 1981; Meiotic mapping of the nuclear determinant of cell lysis of the osmotic dependent Saccharomyces cerevisiae mutant VY 1 160. Molecular and General Genetics 181:414–415
    [Google Scholar]
  30. Venkov P. V., Hadjiolov A. A., Battaner E., Schlezinger D. 1974; Saccharomyces cerevisiae sorbitol dependent fragile mutants. Biochemical and Biophysical Research Communications 56:599–604
    [Google Scholar]
  31. Venkov P. V., Milchev G. I., Hadjiolov A. A. 1975; Rifampicin susceptibility of RNA synthesis in a fragile Saccharomyces cerevisiae mutant. Antimicrobial Agents and Chemotherapy 8:627–632
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-6-1263
Loading
/content/journal/micro/10.1099/00221287-137-6-1263
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error