1887

Abstract

Filaments of lost over half of their gas vesicles when exposed to light of high irradiance (> 115 μmol m s) for long periods (up to 23 h). An investigation using different irradiances showed that the major loss of gas vesicles occurred after prolonged exposure to irradiances exceeding 80 μmol m s. In a time-course experiment it was found that 56% of the gas vesicles were lost after 16 h exposure to an irradiance of 135 μmol m s. Most of this loss could be accounted for by collapse under turgor pressure, which rose by 0·24 MPa to 0·54 MPa after 16 h. A further unexplained loss of 15 % after 23 h exposure may indicate that prolonged high irradiance can cause weakening of gas vesicles in the cells. In this time-course experiment 98% of the filaments lost their buoyancy after 6 h exposure, before any gas vesicles were lost; this buoyancy change was quantitatively accounted for by the rise in cell carbohydrate. The relative importance of gas vesicle collapse and carbohydrate accumulation as mechanisms of buoyancy loss is discussed in relation to vertical movements of planktonic cyanobacteria in lakes.

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1991-05-01
2021-10-19
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References

  1. Allison E. M., Walsby A. E. 1981; The role of potassium in the control of turgor pressure in a gas-vacuolate blue-green alga. Journal of Experimental Botany 32:241–249
    [Google Scholar]
  2. Bligh E. C., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  3. Booker M. J., Walsby A. E. 1979; The relative form resistance of straight and helical blue-green algal filaments. British Phycological Journal 14:141–150
    [Google Scholar]
  4. Booker M. J., Walsby A. E. 1981; Bloom formation and stratification by a planktonic blue-green alga in an experimental water column. British Phycological Journal 16:411–421
    [Google Scholar]
  5. Buckland B., Walsby A. E. 1971; A study of the strength and stability of gas vesicles isolated from a blue-green alga. Archiv fur Microbiologie 79:327–337
    [Google Scholar]
  6. Deacon C., Walsby A. E. 1990; Gas vesicle formation in the dark, and in light of different irradiances, by the cyanobacterium Microcystis sp. British Phycological Journal 25:133–139
    [Google Scholar]
  7. Dinsdale M. T., Walsby A. E. 1972; The interrelations of cell turgor pressure, gas-vacuolation, and buoyancy in a blue-green alga. Journal of Experimental Botany 23:561–570
    [Google Scholar]
  8. Grant N. G., Walsby A. E. 1977; The contribution of photosynthate to turgor pressure rise in the planktonic blue-green alga Anabaena flos-aquae . Journal of Experimental Botany 28:409–415
    [Google Scholar]
  9. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  10. Klemer A. R. 1978; Nitrogen limitation of growth and gas vacuolation in Oscillatoria rubescens . Verhandlungen der Internationalen Vereinigung für Theoretische und Angewandte Limnologie 20:2293–2297
    [Google Scholar]
  11. Klemer A. R., Feuillade J., Feuillade M. 1982; Cyanobacter- ial blooms: carbon and nitrogen limitation have opposite effects on the buoyancy of Oscillatoria . Science 215:1629–1631
    [Google Scholar]
  12. Konopka A. 1982; Buoyancy regulation and vertical migration by Oscillatoria rubescens in Crooked Lake, Indiana. British Phycological Journal 17:427–442
    [Google Scholar]
  13. Konopka A., Brock T. D., Walsby A. E. 1978; Buoyancy regulation by planktonic blue-green algae in Lake Mendota, Wisconsin. Archiv für Hydrobiologie 83:524–537
    [Google Scholar]
  14. Konopka A., Kromkamp J. C., Mur L. R. 1987a; Buoyancy regulation in phosphate-limited cultures of Microcystis aeruginosa . FEMS Microbiology Ecology 45:135–142
    [Google Scholar]
  15. Konopka A., Kromkamp J. C., Mur L. R. 1987b Regulation of gas vesicle content and buoyancy in light- or phosphate-limited cultures of Aphanizomenon flos-aquae (Cyanophyta). Journal oj Phycology 23:70–78
    [Google Scholar]
  16. Kromkamp J. C., Mur L. R. 1984; Buoyant density changes in the cyanobacterium Microcystis aeruginosa due to changes in the cellular carbohydrate content. FEMS Microbiology Letters 25:105–109
    [Google Scholar]
  17. Kromkamp J. C., Konopka A., Mur L. R. 1986; Buoyancy regulation in a strain of Aphanizomenon flos-aquae (Cyanophyceae): the importance of carbohydrate accumulation and gas vesicle collapse. Journal of General Microbiology 132:2113–2121
    [Google Scholar]
  18. Kromkamp J. C., Konopka A., Mur L. R. 1988; Buoyancy regulation in light-limited continuous cultures of Microcystis aeruginosa . Journal of Plankton Research 10:171–183
    [Google Scholar]
  19. Kromkamp J. C., van den Heuvel A., Mur L. R. 1989; Formation of gas vesicles in phosphorus-limited cultures of Microcystis aeruginosa . Journal of General Microbiology 135:1933–1939
    [Google Scholar]
  20. van Liere L., Walsby A. E. 1982; Interactions of cyanobacteria with light. The Biology of the Cyanobacteria9–45 Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  21. Oliver R. L., Walsby A. F. 1984; Direct evidence for the role of light-mediated gas vesicle collapse in the buoyancy regulation of Anabaena flos-aquae (cyanobacteria). Limnology and Oceanography 29:879–886
    [Google Scholar]
  22. Oliver R. L., Walsby A. E. 1988; Buoyancy and suspension of planktonic cyanobacteria. Methods in Enzymology 167:521–527
    [Google Scholar]
  23. Oliver R. L., Kinnear A. J., Ganf G. G. 1981; Measurements of cell density of three freshwater phytoplankters by density gradient centrifugation. Limnology and Oceanography 26:285–294
    [Google Scholar]
  24. Oliver R. L., Utkilen H. C., Walsby A. E. 1982; Mechanismsof buoyancy change in different types of planktonic cyanobacteria. Proceedings of the 4th International Symposium on Photosynthetic Prokaryotes, Bombannes, Abstract C35:
    [Google Scholar]
  25. Oliver R. L., Thomas R. H., Reynolds C. S., Walsby A. E. 1985; The sedimentation of buoyant Microcystis colonies caused by precipitation with an iron-containing colloid. Proceedings of the Royal Society of London B223511–528
    [Google Scholar]
  26. Paerl H. W., Ustach J. F. 1982; Blue-green algal scums: an explanation for their occurrence during freshwater blooms. Limnology and Oceanography 27:212–217
    [Google Scholar]
  27. Reynolds C. S. 1973; Growth and buoyancy of Microcystis aeruginosa Kiitz. emend. Elenkin in a shallow eutrophic lake. Proceedings of the Royal Society of London B18429–50
    [Google Scholar]
  28. Reynolds C. S. 1975; Interrelations of photosynthetic behaviour and buoyancy regulation in a natural population of a blue-green alga. Freshwater Biology 5:323–338
    [Google Scholar]
  29. van Rijn J., Shilo M. 1985; Carbohydrate fluctuations, gas vacuolation and vertical migration of scum-forming cyanobacteria in fishponds. Limnology and Oceanography 30:1219–1228
    [Google Scholar]
  30. Spencer C. N., King D. L. 1985; Interactions between light, NHX, and CO2 in buoyancy regulation of Anabaena flos-aquae (Cyanophyceae). Journal of Phycology 21:194–199
    [Google Scholar]
  31. Thomas R. H., Walsby A. E. 1985; Buoyancy regulation in a strain of Microcystis . Journal of General Microbiology 131:799–809
    [Google Scholar]
  32. Thomas R. H., Walsby A. E. 1986; The effect of temperature on recovery of buoyancy by Microcystis . Journal of General Microbiology 137:1665–1672
    [Google Scholar]
  33. Utkilen H. C., Oliver R. L., Walsby A. E. 1985; Buoyancy regulation in a red Oscillatoria unable to collapse gas vacuoles by turgor pressure. Archiv fur Hydrobiologie 102:319–329
    [Google Scholar]
  34. Walsby A. E. 1970; The nuisance algae: curiosities in the biology of planktonic blue-green algae. Water Treatment and Examination 19:359–373
    [Google Scholar]
  35. Walsby A. E. 1971; The pressure relationships of gas vacuoles. Proceedings of the Royal Society of London B178301–326
    [Google Scholar]
  36. Walsby A. E. 1973; A portable apparatus for measuring relative gas vacuolation, the strength of gas vacuoles, and turgor pressure in planktonic blue-green algae and bacteria. Limnology and Oceanography 18:653–658
    [Google Scholar]
  37. Walsby A. E. 1980; The water relations of gas-vacuolate prokaryotes. Proceedings of the Royal Society of London B20873–102
    [Google Scholar]
  38. Walsby A. E. 1982 The elastic compressibility of gas vesiclesProceedings of the Royal Society of London B216355–368
    [Google Scholar]
  39. Walsby A. E. 1987; Mechanisms of buoyancy regulation by planktonic cyanobacteria with gas vesicles. The Cyanobacteria377–392 Fay P., Van Baalen C. Amsterdam: Elsevier;
    [Google Scholar]
  40. Walsby A. E. 1988; Determination of turgor pressure and other cell- solute relations by using gas vesicles as pressure probes. Methods in Enzymology 167:660–666
    [Google Scholar]
  41. Walsby A. E., Bleything A. 1988; The dimensions of cyanobacterial gas vesicles in relation to their efficiency in providing buoyancy and withstanding pressure. Journal of General Microbiology 134:2635–2645
    [Google Scholar]
  42. Walsby A. E., Booker M. J. 1980; Changes in buoyancy of a planktonic blue-green alga in response to light intensity. British Phycological Journal 15:311–319
    [Google Scholar]
  43. Walsby A. E., Klemer A. R. 1974; The role of gas vacuoles in the microstratification of a population of Oscillatoria agardhii var. isothrix in Deming Lake, Minnesota. Archiv fur Hydrobiologie 74:375–392
    [Google Scholar]
  44. Walsby A. E., Utkilen H. C., Johnsen I. J. 1983; Buoyancy changes of a red coloured Oscillatoria agardhii in Lake Gjersjoen, Norway. Archiv fur Hydrobiologie 97:18–38
    [Google Scholar]
  45. Walsby A. E., Reynolds C. S., Oliver R. L., Kromkamp J. 1989; The role of gas vacuoles and carbohydrate content in the buoyancy and vertical distribution of Anabaena minutissima in Lake Rotongaio, New Zealand. Archiv fur Hydrobiologie . Ergebnisse der Limnologie 32:1–25
    [Google Scholar]
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