3†Present address: Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina, Chapel Hill, NC 27599-7400, USA.
The concentration of the type 5 capsular polysaccharide (CP) antigen of Staphylococcus aureus can be measured directly in cultures or cell suspensions by a two-step inhibition enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies. CP was synthesized during growth on a variety of carbon substrates and its production was not affected by the nature of the carbon source. High levels of yeast extract inhibited CP formation. CP was synthesized in batch culture at the same rate during exponential growth as in the post-exponential phase. Post-exponential CP production contributed at least half the final amount of CP measured. This phenomenon was observed in different culture media, although the specific yield of polysaccharide varied from one medium to another. Post-exponential CP production was observed in the pH range 6–7, but not at pH 8. Post-exponential production was strictly dependent on oxygen availability and did not occur under anaerobic conditions.
AlbusA.,
FournierJ. M.,
WolzC.,
BoutonnierA.,
RankeM.,
HøbyN.,
HochkeppelH.,
DoringG.1988; Staphylococcus aureus capsular types and antibody response to lung infection in patients with cystic fibrosis. Journal of Clinical Microbiology 26:2505–2509
AndersonP.,
PittJ.,
SmithD. H.1976; Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro
. Infection and Immunity 13:581–589
ArbeitR. D.,
KarakawaW. W.,
VannW. F.,
RobbinsJ. B.1984; Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus
. Diagnostic Microbiology and Infectious Diseases 2:85–91
BoutonnierA.,
NatoF.,
BouvetA.,
LebrunL.,
AudurierA.,
MazieJ. C.,
FournierJ. M.1989; Direct testing of blood cultures for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus
. Journal of Clinical Microbiology 27:989–993
CaputyG. G.,
CostertonJ. W.1984; Immunological examination of the glycocalyces of Staphylococcus aureus strains Wiley and Smith. Current Microbiology 11:297–302
CheungA. L.,
FischettiV. A.1988; Variation in the expression of cell wall proteins of Staphylococcus aureus grown on solid and liquid media. Infection and Immunity 56:1061–1065
ChomaratM.,
IchimanY.,
YoshidaK.1989; Protection of mice by a pseudodiffuse strain of Staphylococcus aureus possessing polyvalent capsular type antigen. Journal of Medical Microbiology 28:129–136
ColemanG.1985; A comparison of the patterns of extracellular proteins produced by the high a-toxin-secreting organism Staphylococcus aureus (Wood 46) during aerobic and anaerobic growth. Journal of General Microbiology 131:405–408
ColemanG.,
JakemanC. M.,
MartinN.1978; Patterns of total extracellular protein secretion by a number of clinically isolated strains of Staphylococcus aureus
. Journal of General Microbiology 107:189–192
DunicanL. K.,
SeeleyH. W.1965; Extracellular polysaccharide synthesis by members of the genus Lactobacillus: conditions for formation and accumulation. Journal of General Microbiology 40:297–308
FalcieriE.,
VaudauxP.,
HugglerE.,
LewD.,
WaldvogelF.1987; Role of bacterial exopolymers and host factor on adherence and phagocytosis of Staphylococcus aureus in foreign body infection. Journal of Infectious Diseases 155:524–531
FournierJ. M.,
HannonK.,
MoreauM.,
KarakawaW. W.,
VannW. F.1987; Isolation of type 5 capsular polysaccharide from Staphylococcus aureus
. Annales de I’Institut Pasteur/Microbiologie 138:561–567
GottschlichE. C.,
Yung LiuT.,
ArtensteinM. S.1969; Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides. Journal of Experimental Medicine 129:1349–1365
HochkeppelH. K.,
BraunD. G.,
VischerW.,
ImmA.,
SutterJ.,
StaeubliU.,
GuggenheimR.,
KaplanE. L.,
BoutonnierA.,
FournierJ. M.1987; Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide type 5 and 8. Journal of Clinical Microbiology 25:526–530
HuberM. M.,
HuberT. W.1989; Susceptibility of methicillin-resistant Staphylococcus aureus to lysostaphin. Journal of Clinical Microbiology 27:1122–1124
JarvisA. W.,
LawrenceR. C.,
PritchardG. G.1973; Production of staphylococcal enterotoxins A, B and C under conditions of controlled pH and aeration. Infection and Immunity 7:847–854
JohneB.,
JarpJ.,
HaaheimL. R.1989; Staphylococcus aureus exopolysaccharide in vivo demonstrated by immunomagnetic separation and electron microscopy. Journal of Clinical Microbiology 27:1631–1635
KarakawaW. W.,
FournierJ. M.,
VannW. F.,
ArbeitR.,
SchneersonR. S.,
RobbinsJ. B.1985; Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus
. Journal of Clinical Microbiology 22:445–447
KellerG. M.,
HansonR. S.,
BergdollM. S.1978; Molar growth yields and enterotoxin B production of Staphylococcus aureus S-6 with amino acids as energy sources. Infection and Immunity 20:151–157
KitzrowD.,
BlobelH.,
SchaegW.1975; Isolation of capsular substances from Staphylococcus aureus
. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Series A) 233:305–313
LeeJ. C.,
BetleyM. J.,
HopkinsC. A.,
PerezN. E.,
PierG. B.1987; Virulence studies, in mice, of transposon-induced mutants of Staphylococcus aureus differing in capsule size. Journal of Infectious Diseases 156:741–750
MassonL.,
HolbeinB. E.1985; Influence of nutrient limitation and low pH on serogroup B Neisseria meningitidis capsular polysaccharide level: correlation with virulence for mice. Infection and Immunity 47:465–471
MattilaT.1989; A staining technique to measure capsular polysaccharides of Staphylococcus aureus on filter membranes. Journal of Microbiological Methods 9:323–331
OpdebeeckJ. P.,
O’BoyleD.,
FrostA. J.1985; The expression of capsule in serum-soft agar by Staphylococcus aureus isolated from human clinical sources. Journal of Medical Microbiology 19:275–278
OpdebeeckJ. P.,
WatsonD. L.,
FrostA. J.1988; Colony morphology of Staphylococcus aureus in serum-soft agar following in vivo and in vitro growth. Veterinary Microbiology 16:87–91
PoutrelB.,
BoutonnierA.,
SutraL.,
FournierJ. M.1988; Prevalence of capsular polysaccharide types 5 and 8 among Staphylococcus aureus isolates from cow, goat, and ewe milk. Journal of Clinical Microbiology 26:38–40
RatherP. N.,
DavisA. P.,
WilkinsonB. J.1986; Slime production by bovine milk Staphylococcus aureus and identification of coagulase-negative staphylococcal isolates. Journal of Clinical Microbiology 23:858–862
RobertsI. S.,
SaundersF. K.,
BoulnoisG. J.1989; Bacterial capsules and interactions with complement and phagocytes. Biochemical Society Transactions 17:462–464
SchlievertP. M.,
BlomsterD. A.1983; Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. Journal of Infectious Diseases 147:236–242
SompolinskyD.,
SamraZ.,
KarakawaW. W.,
VannW. F.,
SchneersonR.,
MalikZ.1985; Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. Journal of Clinical Microbiology 22:828–834
SuhI. S.,
HerbestH.,
SchumpeA.,
DeckwerW. D.1990; The molecular weight of xanthan polysaccharide produced under oxygen limitation. Biotechnology Letters 12:201–206
TurnerW. H.1978; The effect of medium volume and yeast extract diffusate on delta-hemolysin production by five strains of Staphylococcus aureus
. Journal of Applied Bacteriology 45:291–296
WileyB. B.1968; Capsule size, coagulase production, and egg virulence among certain strains of Staphylococcus aureus
. Canadian Journal of Microbiology 14:685–689
WilliamsA. G.,
WimpennyW. T.1980; Extracellular polysaccharide biosynthesis by Pseudomonas NCIB 11264. Studies on precursor-forming enzymes and factors affecting exopolysaccharide production by washed suspensions. Journal of General Microbiology 116:133–141
WitteW.1975; Capsule formation in Staphylococcus aureus as a reason for nontypability by phages. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Series A) 233:447–451
YoshidaK.,
EkstedtR. D.1968; Relation of mucoid growth of Staphylococcus aureus to clumping factor reaction, morphology in serum-soft agar, and virulence. Journal of Bacteriology 96:902–908