1887

Abstract

The 5′ regulatory region and the portion of the structural gene coding for the amino-terminal sequence of alkaline phosphatase I (APase I) were isolated from MC14 using a synthetic oligodeoxynucleotide deduced from the amino acid sequence of the enzyme. The DNA sequence analysis of this region revealed an open reading frame of 129 amino acids containing the amino-terminal sequence of the mature APase protein. The protein sequence was preceded by a putative signal sequence of 32 amino acid residues. The predicted amino acid sequence of the partial APase clone as well as the experimentally determined amino acid sequence of the enzyme indicated that APase retains the important features conserved among other APases of and various human tissues. Heterologous expression studies of the promoter using a fusion with the gene indicated that it functions as a very strong inducible promoter in that is tightly regulated by phosphate concentration.

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/content/journal/micro/10.1099/00221287-137-5-1127
1991-05-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-5-1127
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